Molecular characterization of estrogen receptor genes in loach Paramisgurnus dabryanus and their expression upon 17α-ethinylestradiol exposure in juveniles

The full-length cDNAs for estrogen receptor 1 (esr1), esr2a and esr2b were isolated and characterized from the loach (Paramisgurnus dabryanus, Cobitidae, cypriniformes). P. dabryanus Esr1, Esr2a and Esr2b share high amino acids identities with their counterparts of cyprinid species. Quantitative real-time PCR (qRT-PCR) was used to analyze the tissue distribution of esr mRNAs in one-year-old P. dabryanus. The mRNA expression of esr1 in female liver was extremely higher than that in other tissues. esr2a mRNA expression in female intestine and in male muscle was higher than that in other tissues. esr2b mRNA expression was the highest in both male and female intestine. Two-month-old P. dabryanus were exposed to 17α-ethinylestradiol (EE2) for 3weeks and the changes of esr mRNA expression in brain, gonad and liver were analyzed by qRT-PCR. Results showed that EE2 at 1, 5 and 25ng/L significantly suppressed testicular esr1 mRNA expression in male. The ovarian esr2a mRNA expression was significantly up-regulated at 1ng/L EE2. In female brain, esr1 mRNA expression was significantly down-regulated at 5ng/L EE2. Both in males and females, EE2 exposure increased the hepatic esr1 mRNA expression in a concentration-dependent manner. The present study suggests that different esrs in different tissues have differential responsiveness to EE2 and the hepatic esr1 is a sensitive biomarker to EE2 at environmental concentrations in P. dabryanus juveniles. So, the loach P. dabryanus, a typical demersal fish, is a promising ecological model organism to detect estrogenic chemicals in the sediment of aquatic environment by using molecular biomarkers.     

三种新型可聚合抗菌单体对口腔病原菌的抗菌活性研究

目的:比较自行合成的3种可聚合季铵盐抗菌单体DMAE-MMA salt,DMAEMA-MMA salt,DMAEMA-PMDPM salt对口腔常见致病菌的抗菌活性。方法:采用液体稀释法测定季铵盐单体对4种口腔常见致病菌的抗菌活性,以抗菌性能最佳的单体进行杀菌动力学研究。结果:3种季铵盐单体对4种口腔致病菌均表现出一定的抗菌活性,其中DMAEMA-PMDPM salt抗菌效果较其它2种好,该单体在浓度为78.8 mg/ml即4倍MBC浓度时,白色念珠菌与其接触120 min时可达到灭菌效果。结论:这3种季铵盐单体对常见口腔致病菌均表现出一定的抗菌活性。     

牙周支持治疗阶段患者依从性影响因素分析及对策

目的:探讨慢性牙周炎患者牙周支持治疗阶段依从性影响因素及其对策.方法:自行制订调查问卷,对已完成牙周非手术基础治疗,且已进入牙周支持治疗阶段(SPT)的慢性牙周炎患者,2a后通过门诊随访和电话回访获取资料,进行分析.结果:共回收有效问卷101份,相关因素分析显示,疾病知晓度、医疗服务质量、年龄、文化程度、经济状况等多种因素可影响依从性.结论:影响慢性牙周炎患者依从性的因素较多,提高患者依从性的关键在于有针对性地对患者进行口腔健康教育、定期随访和改善医疗服务质量.