停跳或不停跳心脏手术对血清 S-100B蛋白表达的影响

【目的】研究心脏手术围术期血清S-100B蛋白表达及其与停跳或不停跳心肺转流方式和时间的关系。【方法】体外循环心脏手术患者23例,测转流前、转流10min、转流末、转流后24h的血清S-100B蛋白表达水平。【结果】①血清S-100B蛋白质量浓度在体外循环前后动态变化:转流前(M)为0.27μg/L,转流10min后升至0.57μg/L(P<0.01),转流末达峰值1.80μg/L(P<0.01),转流后24h降为0.22μg/L(P>0.05)。转流末的血清S-100B蛋白质量浓度与转流时间呈正相关(r=0.488,P<0.05)。②停跳组(n=6)转流前、转流10min、转流末、转流后24h平均血清S-100B蛋白质量浓度分别为(0.17±0.09)μg/L、(0.48±0.13)μg/L、(1.65±0.52)μg/L和(0.19±0.04)μg/L,不停跳组(n=6)分别为(0.26±0.14)μg/L、(0.71±0.41)μg/L、(1.59±0.84)μg/L和(0.23±0.11)μg/L,两组差别无统计学意义(P>0.05)。【结论】体外循环可导致血清S-100B蛋白表达增高,其表达水平与心肺转流时间呈正相关,但与停跳或不停跳转流方式无关。     

微创治疗迟发性脑内血肿(附78例报告)

目的：评价微创治疗迟发性脑内血肿的疗效。方法：采取锥颅穿刺置管 尿激酶灌注引流方法，回顾分析78例迟发脑内血肿的临床资料。结果：78例迟发脑内血肿经微创治疗后存活71例，死亡5例，总死亡率6％。格拉斯哥昏迷指数(GCS)、年龄、瞳孔变化、血肿大小、脑挫伤程度及并发症等6项指标可影响治疗效果。结论：迟发性脑内血肿采取微创治疗具有安全性、少创、经济、恢复时间短、并发症少、死亡率低等优点。是一种有效可靠的方法。     

心肌缺血再灌注损伤时细胞核被膜钙泵活性特征的研究

目的　研究心肌缺血再灌注损伤时Ca2 + 、ATP、ADP和AMP对细胞核被膜钙泵 (Ca2 + ATPase)活性的调节。方法　采用大鼠心肌缺血再灌注模型 ,密度梯度分离纯化细胞核 ,定磷法测定Ca2 + ATPase活性。结果　与正常大鼠相比 ,缺血再灌注损伤动物血浆MDA和FFA显著升高 (P <0 0 1) ;细胞核Ca2 + ATPase活性下降 ,对Ca2 + 亲和力降低 ;ADP、AMP对核Ca2 + ATPase活性的影响明显减弱 ;ATP对Ca2 + ATPase的作用在 [ATP]小于 1 0mmol L时 ,与正常细胞Ca2 + AT Pase活性无显著差异 ,浓度增至 2 0mmol L时 ,活性低于正常细胞 ,ATP浓度继续增加 ,核Ca2 + ATPase活性快速增加。结论　心肌缺血再灌注损伤时Ca2 + 、ATP、ADP、AMP对心肌细胞核Ca2 + ATPase活性的影响发生了明显改变 ,其病理生理意义值得进一步探讨。     

杆状病毒介导LacZ基因转染体外培养的人虹膜色素上皮细胞

目的：研究重组杆状病毒(baculovinus)载体介导β—半乳糖苷酶基因(LacZ)对体外培养虹膜色素上皮(IPE)细胞的转染和表达情况。方法：将质粒pCMV—β中的CMV—LacZ表达盒插入杆状病毒表达栽体pFast BacI的Eco RI／Hind Ⅲ位点，构建重组质粒pBac—β，并利用Bac—to—Bac表达系统在Sf9细胞中产生出重组杆状病毒BV—β。将MOI为200的BV—β感染经酶消化加显微法分离培养的IPE细胞，24h后进行X-gal染色。在显微镜下观察IPE中LacZ表达阳性的情况，记录阳性细胞所占的百分比。结果：限制性酶切分析及测序结果表明，我们已成功构建表达质粒pBac—β及重组杆状病毒BV—β。X-gal染色证实杆状病毒介导LacZ在细胞中的表达呈蓝色，弥漫于整个胞浆。感染后24h时表达阳性率达85％。结论：重组杆状病毒可有效介导报告基因在体外培养的IPE细胞中表达，为利用杆状病毒构建可供移植的基因工程化虹膜色素上皮细胞提供了实验依据。     

Electrical impedance scanning in breast tumor imaging: correlation with the growth pattern of lesion

Background This study researched the electric impedance properties of breast tissue and demonstrated the differentcharacteristic of electrical impedance scanning (EIS) images.Methods The impedance character of 40 malignant tumors, 34 benign tumors and some normal breast tissue from 69patients undergoing breast surgery was examined by EIS in vivo measurement and mammography screening, with aseries of frequencies set between 100 Hz-100 kHz in the ex vivo spectroscopy measurement.Results Of the 39 patients with 40 malignant tumors, 24 showed bright spots, 11 showed dark areas in EIS and 5showed no specific image. Of the 30 patients with 34 benign tumors there were almost no specific abnormality shown inthe EIS results. Primary ex vivo spectroscopy experiments showed that the resistivity of various breast tissue take thefollowing pattern: adipose tissue＞cancerous tissue＞mammary gland and benign tumor tissue.Conclusions There are significant differences in the electrical impedance properties between cancerous tissue andhealthy tissue. The impedivity of benign tumor is lower, and is at the same level with that of the mammary glandulartissue. The distinct growth pattern of breast lesions determined the different electrical impedance characteristics in theEIS results.     

大鼠脂肪干细胞复合胶原-壳聚糖-硫酸软骨素三维支架构建组织工程软骨

Objective To evaluate the character of the collagen-chitosan-chondroitin sulfate scaffold seeded with rat adipose tissue-derived stromal cells. Methods A dipose tissue were harvested from 6 weeks old Wistar rats and the stromal cells were harvested by type Ⅰ collagenase and then cultured in vitro. Type Ⅰ collagen was fully mixed with chitosan, freeze-dried and cross-linked with chondroitin sulfate, then freeze-dried again and sterilized by ethylene oxide. The pore diameter, water content, porosity of the scaffold were tested. The adipose tissue-derived stromal cells were digested, seeded into the plates, scaffold, and cen-trifuged into pellet, and then induced into cartilage. MTT detection for cell proliferation was done. After 3 weeks, the cell morphology, and cell proliferation and adhesion were observed, and chondrngenic differenti-ation was also analyzed. Results The pore diameter, water content, porosity tested for the scaffold showed an appropriate form. Cell proliferation showed faster in the scaffold and pellet culture system after 5 day, there was still cell proliferation in the scaffold system after 14 days but no obvious changes in the pellet cul-ture system; ceils on the scaffold proliferated densely showed by histological staining, but there was a scaf-fold structure residues in the inner layer. The finding of type Ⅱ immunohistochemistry stain showed that cells express strong positive for type Ⅱ collagen in the scaffold and pellet culture system whereas it was weakly positive in the plate culture system; the specific mRNA for cartilage, type Ⅱ collagen, aggrecan and SOX-9 were expressed in all three systems showed by RT-PCR, but type X collagen was expressed continu-ously in the plate culture system and expressed after 21 days in the pellet culture system, whereas it was not detected in the collagen-chitosan-chondroitin sulfate scaffold system. Conclusion The parameters of the collagen-chitosan-chondroitin sulfate scaffold were suitable in our study. The results suggested that it can promote the adipose tissue-derived stromal cells proliferation and chondrogenic differentiation better than the plate and pellet culture systems and maintain the phenotype of chondrocytes well; it is the optimal choice for cartilage tissue engineering in the future.     

中性粒细胞分布数据序列小波变换研究

目的　研究小波分析结合复倒谱处理血膜中中性粒细胞分布数据信号的新方法及临床意义。方法　利用“虚拟盒计数法”方法采集血膜中的细胞分布数据信号 ,然后进行小波变换结合复倒谱分析方法处理。结果　提出血膜中细胞分布状态的“密集子”新概念。计算出小波变换复倒谱主极大值 ,并以此作为不同情况下血膜中中性粒细胞分布“密集子”间距的准确参数。结论　方法简单 ,能较好的反映血膜中细胞分布状态的微观组织结构特征 ,是血膜中细胞“密集子”表达定征的一种有效方法     

神经营养素受体在人外周血T细胞中表达的研究

目的 研究人外周血CD4^ /CD8^ T细胞4种神经营养素受体基因的转录。方法 应用尼龙手法分离出T细胞，磁式细胞分离法（MACS）分离CD4^ /CD8^ T细胞亚群，再以RT－PCR法研究4种神经营养素受体在两种T细胞亚群上的表达。结果 未经刺激的CD4^ /CD8^ T细胞亚群不表达任何神经营养素受体。经PHA或PPD刺激后，CD4^ /CD8^ T细胞亚群表达trkA,CD8^ T细胞亚群表达trkC；而在各种状态下的T细胞上均未见表达trkB及p75^NGFR。结论神经营养素受体在两种T细胞亚群中有不同的表达格局，提示不同T细胞亚群受神经营养素调节的模式可能各不相同。     

模拟退火算法在图像配准中的应用

近年来 ,脑功能成像技术发展迅速 ,已在脑科学研究中占有重要地位。研究中通常需要将同一被试的多种模式成像结果或同一模式的时间序列结果结合起来分析 ,首先要解决的问题就是几幅图像的严格对齐问题 ,即所谓的图像配准。在模拟退火算法和其它工作的基础之上 ,较好的解决了图像配准中的连续变量全局优化问题 ,通过基于点的配准方法 ,实现了时间序列脑功能图像的高精度配准。     

胰岛素样生长因子(IGF)与子宫内膜异位症关系的研究进展

本文对IGF家族的组成、结构、在多种肿瘤中的作用机制及其在内异症中的研究进展作一综述,旨在为内异症的诊治提供新的依据。其中研究较明确的是IGF-I和IGFBP-3。IGF-I促进甾体激素的生成、促进细胞增殖、抑制细胞凋亡,与多种肿瘤的发生有关。IGF-I促进异位子宫内膜生长,与内异症形成有关。IGFBP-3作用与IGF-I相反,可能是通过与IGF-I的结合而实现。