The effects of sex and hormonal status on restraint-stress-induced working memory impairment

Background  

Restraint stress has been shown to elicit numerous effects on hippocampal function and neuronal morphology, as well as to induce dendritic remodeling in the prefrontal cortex (PFC). However, the effects of acute restraint stress on PFC cognitive function have not been investigated, despite substantial evidence that the PFC malfunctions in many stress-related disorders.     

Effect of Serum pH on Bilirubin-Protein Binding

Progressive increase of the serum pH upon standing and storage in different conditions is documented. Bilirubin albumin titration studies were performed on serum of different pH by Sephadex gel filtration and horseradish peroxidase oxidation methods. Results showed that different pH produced different titration curves and that more reproducible and consistent results were obtained with the peroxidase oxidation titration method. Addition of HC1 and CO₂ to the serum also produced different bilirubin-protein titration results. For blirubin albumin binding studies, we suggest to adjust the pH to 7.4 with CO₂ to obtain clinically meaningful data.