黄芪散微丸对糖尿病肾病大鼠肾脏PI3K/Akt/mTOR通路及自噬的影响

目的:研究黄芪散微丸(HQS)对糖尿病肾病(DN)大鼠肾脏磷脂酰肌醇3-激酶/蛋白激酶B/哺乳动物雷帕霉素靶蛋白(PI3K/Akt/mTOR)信号通路的影响,探讨其对自噬的影响.方法:采用高脂饲料喂养联合腹腔注射链脲佐菌素(35mg·kg-1)建立DN大鼠模型.DN大鼠随机分为模型组、厄贝沙坦(0.027g·kg-1)...     

股骨颈骨折治疗的不同术式比较

目的 评价双头空心加压螺钉内固定术和人工股骨头置换术治疗老年股骨颈骨折患者的疗效.方法 股骨颈骨折患者70例,随机分成双头空心加压螺钉内固定术组和人工股骨头置换术组两组,比较两种治疗方案的疗效和安全性.结果 两组均能很好治疗股骨颈骨折患者,但是采用双头空心加压螺钉内固定术的治疗组髋关节功能优良率（54.3%）要明显高于人工股骨头置换术组（28.6%）.两组患者住院及康复期间均未发生褥疮、坠积性肺炎等并发症.结论 采用双头空心加压螺钉内固定术治疗老年股骨颈骨折患者不但疗效佳,而且也相对安全.     

高效液相色谱法测定硫辛酸注射液中有关物质及其含量

目的：建立以高效液相色谱法测定硫辛酸注射液中有关物质及其含量的方法。方法：色谱柱为ShimpackCLC-ODS（5μm,150.0mm×4.6mm）不锈钢柱,流动相为乙腈—0.005mo1/L磷酸二氢钾溶液（50∶50,用磷酸调pH为3.0）,流速为1.0ml/min,进样量为20μl,检测波长为212nm。结果：硫辛酸进样量在0.81～8.10μg的范围内线性关系良好（r=0.999）;平均回收率为99.17%（RSD=0.40%）。结论：本方法简便、准确,可用于硫辛酸注射液的有关物质及其含量的测定。     

酸性染料比色法测定延胡索总生物碱的含量

目的探讨延胡索中总生物碱含量测定的方法。方法采用酸性染料比色法测定延胡索中总生物碱含量。结果酸性染料比色法的显色条件为pH 4.4缓冲液12.0 mL,溴甲酚绿液3.0 mL,氯仿9mL萃取一次,振摇2 min,静置40 min。生物碱在1.33μg/mL～11.56μg/mL范围内呈线性关系,回收率平均值100.4%,,RSD=1.01%(n=6)。结论本含量测定方法操作简单,灵敏度高,准确度好,重现性好,可用于测定延胡索总生物碱的含量。     

Single‐Catheter Technique for Pulmonary Vein Antrum Isolation: Is It Sufficient to Identify and Close the Residual Gaps Without a Circular Mapping Catheter?

Objectives: The present study was designed to investigate the feasibility and efficacy of single ablation catheter for complete circumferential pulmonary vein antrum (PVA) isolation.
Background: Complete isolation of pulmonary veins is the mainstay for atrial fibrillation (AF) ablation. This is usually performed under the guidance of a circular catheter.
Methods: One hundred and ten consecutive patients with paroxysmal AF were prospectively randomized into two groups: single-catheter approach (group 1) and double-catheter approach (group 2). After performing initial circumferential lesions, residual gaps were mapped and closed with single ablation catheter in group 1 or guided by a circular mapping catheter in group 2 using an electroanatomic mapping system (CARTO™ XP, Biosense-Webster Inc., Diamond Bar, CA, USA).
Results: Complete bilateral PVA isolation was achieved in 22 of the 110 patients after initial ablation. All residual gaps could be correctly identified by activation mapping using single ablation catheter. The distribution of these residual gaps was asymmetric. In group 1, 25 gaps along the right PVA lesions and 49 gaps along the left PVA lesions were identified. All the residual gaps were closed with single-catheter approach. In group 2, 28 gaps on the right side and 53 gaps on the left side were identified using a circular catheter and closed with further ablations. The procedure data and clinical outcomes between the two groups were comparable.
Conclusions: Single ablation catheter technique is feasible and as effective as circular catheter mapping in localizing the residual gaps for PVA isolation during ablation of paroxysmal AF.     

Functional ENaC Channels Expressed in Endothelial Cells: a New Candidate for Mediating Shear Force

Endothelial cells (ECs) are regulated not only by circulating hormones, but also by mechanical stresses, such as shear force. Ion channels in ECs can signal rapid changes of shear forces and are involved in controling EC permeability, proliferation, and angiogenesis. In this study, we employed patch clamping and molecular biology approaches to clarify whether the epithelial sodium channel (ENaC) is functionally expressed in ECs. The α‐subunit of the ENaC was expressed in cultured human ECs and in intact ECs from a variety of rat arteries. In either inside‐ or outside‐out current recordings, inward currents with a conductance of 4.83 pS were detected in cultured human ECs, where these were sensitive to micromolar amiloride. The right shift of the I‐V curve in the condition of low cytoplasmic Na⁺ implicated that these currents were carried by Na⁺. The currents were mediated by ENaC channels, as confirmed by ENaC knockdown experiments. However, the activity of ENaC was nearly absent in intact ECs, because its activity was greatly inhibited by cellular molecules, partly due to 11,12‐epoxyeicosatrienoic acid. In the outside‐out configuration, laminar flow directly enhanced ENaC opening probability, suggesting a potential role for ENaC in mediating shear force signaling events.     

急性肺血栓栓塞症57例临床特点分析

目的分析肺血栓栓塞症（PTE）的临床特点,提高其早期诊断率。方法回顾性分析57例PTE患者的临床特点。结果PTE患者临床表现缺乏特异性,常伴有深静脉血栓形成、创伤和（或）骨折、心血管疾病等危险因子;动脉血气分析、D-二聚体、心电图检查可提示PTE;超声心动图对PTE有诊断作用;螺旋CT肺动脉血管造影（CTPA）是确诊PTE较可靠的方法。结论PTE是常见的肺血管疾病,对有症状的高危患者应及早行CTPA检查,以明确诊断。     

重症急性胰腺炎的早期综合治疗

目的探讨重症急性胰腺炎的早期综合治疗方法,以提高治愈率,降低并发症和死亡率。方法回顾分析28例重症急性胰腺炎的诊治过程,分析早期综合治疗的临床疗效。结果24例治愈,4例死亡;连续性血液净化组早期临床表现改善明显。结论重症急性胰腺炎的早期个体化综合治疗尤为重要,早期阻断全身性炎症反应综合症的发生能明显改善患者的预后。     

成骨生长肽对不同血清浓度下OPG-/-小鼠骨髓基质细胞增殖和分化作用

 目的 研究合成成骨生长肽（synthetic osteogenic growth peptide,sOGP） 对在不同血清浓度条件下OPG-/-小鼠骨髓基质细胞（bone marrow derived stroma cells,BMSCs）的增殖和分化作用。方法 取OPG-/-小鼠股骨,分离骨髓基质细胞进行体外培养。在各自培养条件下分为实验组（OGP组）、空白对照组（CON组）和阳性对照组（bone morphogenetic protein-2 group,BMP-2组）,倒置相差显微镜下观察细胞形态变化、MTT法测定细胞增殖率、PNPP法测定碱性磷酸酶（alkaline phosphatase,ALP）的表达。结果 OGP在含10%胎牛血清（fetal bovine serum,FBS）的LG-DMEM和矿化液的培养条件下,对OPG-/-小鼠BMSCs 起到增殖作用,在第3、5天均高于CON组和BMP-2组（P<0.05）;OGP组对ALP表达的影响与CON组相似,而与BMP-2组存在较大差异,BMP-2组在所有不同血清浓度条件下的第3、5天ALP活性均高于CON组和OGP组（P<0.05）。结论 OGP组对OPG-/-小鼠BMSCs的增殖作用要求较高血清浓度的体外培养条件,但在ALP的分化活性方面弱于BMP-2组。     

子宫内膜异位症患者经血、血清、腹腔液中血管内皮生长因子的检测及其诊断意义

目的探讨经血、血清、腹腔液血管内皮生长因子（VEGF）检测在子宫内膜异位症（EMs）诊断中的意义。方法采用酶联免疫吸附（ELISA）法测定36例EMs患者经血、血清、腹腔液VEGF水平,以同期因输卵管阻塞而行手术者25例作为对照组。结果 （1）EMs组经血中VEGF水平显著高于对照组（P〈0.01）,EMs腹腔液中VEGF水平高于对照组（P〈0.01）;两组血清VEGF相比,差异无统计学意义（P〉0.05）。（2）EMs组腹腔液中VEGF水平与EMs分期成正相关（r=0.93,P〈0.05）,而血清和经血中VEGF水平与分期无关（r=0.108和0.147,均P〉0.05）。结论子宫内膜异位症患者经血中VEGF水平显著高于正常妇女,与分期无关。经血中VEGF的检测具有方便、无创、价廉、依从性好等优点,可望成为诊断EMs的生物学指标。