全文获取类型
收费全文 | 620篇 |
免费 | 52篇 |
国内免费 | 1篇 |
专业分类
儿科学 | 17篇 |
妇产科学 | 8篇 |
基础医学 | 145篇 |
口腔科学 | 5篇 |
临床医学 | 46篇 |
内科学 | 111篇 |
皮肤病学 | 17篇 |
神经病学 | 59篇 |
特种医学 | 14篇 |
外科学 | 66篇 |
综合类 | 2篇 |
预防医学 | 77篇 |
眼科学 | 12篇 |
药学 | 52篇 |
中国医学 | 2篇 |
肿瘤学 | 40篇 |
出版年
2023年 | 5篇 |
2022年 | 16篇 |
2021年 | 11篇 |
2020年 | 9篇 |
2019年 | 13篇 |
2018年 | 15篇 |
2017年 | 12篇 |
2016年 | 21篇 |
2015年 | 26篇 |
2014年 | 31篇 |
2013年 | 33篇 |
2012年 | 60篇 |
2011年 | 54篇 |
2010年 | 31篇 |
2009年 | 32篇 |
2008年 | 40篇 |
2007年 | 34篇 |
2006年 | 31篇 |
2005年 | 34篇 |
2004年 | 15篇 |
2003年 | 18篇 |
2002年 | 22篇 |
2001年 | 12篇 |
2000年 | 10篇 |
1999年 | 9篇 |
1998年 | 7篇 |
1997年 | 13篇 |
1996年 | 4篇 |
1995年 | 3篇 |
1994年 | 6篇 |
1992年 | 4篇 |
1991年 | 7篇 |
1990年 | 3篇 |
1989年 | 3篇 |
1984年 | 2篇 |
1983年 | 1篇 |
1979年 | 2篇 |
1978年 | 1篇 |
1977年 | 1篇 |
1976年 | 1篇 |
1975年 | 1篇 |
1974年 | 2篇 |
1971年 | 2篇 |
1969年 | 3篇 |
1968年 | 2篇 |
1967年 | 2篇 |
1966年 | 2篇 |
1965年 | 1篇 |
1949年 | 1篇 |
1920年 | 1篇 |
排序方式: 共有673条查询结果,搜索用时 15 毫秒
21.
22.
Randomised clinical study: the effects of oral taurine 6g/day vs placebo on portal hypertension 下载免费PDF全文
23.
Yorgos Luiz Santos De Salles Gra?a Ana Cristina Opolski Barbara Evelin Gon?alves Barboza Bruna Olandoski Erbano Caroline Cantalejo Mazzaro Flávia Caroline Klostermann Enéas Eduardo Sucharski Luiz Fernando Kubrusly 《Brazilian Journal Of Cardiovascular Surgery》2014,29(2):272-278
Objective
The aim of the present investigation was to determine whether the difference in inflammatory tissue reaction between the Riccinus communis (castor) polymer with calcium carbonate and the titanium implant is statistically significant.Methods
Thirty-two Cavia porcellus were allocated into four groups of eight animals each. We implanted the two types of materials in the retroperitoneal space of all the animals. They were euthanized at 7, 20, 30 and 40 days after surgery, and an histological study of the samples was conducted.Results
All implants showed characteristics of chronic inflammation regardless of the material and timepoint of evaluation. There was no statistically significant difference between Pm+CaCO3 and Ti with regard to the presence of granulation tissue, tissue congestion, histiocytes, lymphocytes, neutrophils, giant cells, and fibrosis (P> 0.05).Conclusion
The castor oil polymer plus calcium carbonate implant was not statistically different from the titanium implant regarding inflammatory tissue reaction. 相似文献24.
The bacterial and archaeal cell surface is decorated with filamentous surface structures that are used for different functions, such as motility, DNA exchange and biofilm formation. Viruses hijack these structures and use them to ride to the cell surface for successful entry. In this review, we describe currently known mechanisms for viral attachment, translocation, and entry via filamentous surface structures. We describe the different mechanisms used to exploit various surface structures bacterial and archaeal viruses. This overview highlights the importance of filamentous structures at the cell surface for entry of prokaryotic viruses. 相似文献
25.
Enn K Seppet Margus Eimre Tiia Anmann Evelin Seppet Nadezhda Peet Tuuli Kmbre Kalju Paju Andres Piirsoo Andrei V Kuznetsov Marko Vendelin Frank N Gellerich Stephan Zierz Valdur A Saks 《Experimental & Clinical Cardiology》2005,10(3):173-183
BACKGROUND:
The present review examines the role of intra-cellular compartmentation of energy metabolism in vivo.OBJECTIVE:
To compare the kinetics of the activation of mitochondrial respiration in skinned cardiac fibres by exogenous and endogenous adenine nucleotides in dependence of the modulation of cellular structure and contraction.METHODS:
Saponin-permeabilized cardiac fibres or cells were analyzed using oxygraphy and confocal microscopy.RESULTS:
Mitochondria respiration in fibres or cells was upregulated by cumulative additions of ADP to the medium with an apparent Km of 200 μM to 300 μM. When respiration was stimulated by endogenous ADP produced by intracellular ATPases, a near maximum respiration rate was achieved at an ADP concentration of less than 20 μM in the medium. A powerful ADP-consuming system, consisting of pyruvate kinase and phosphoenolpyruvate, that totally suppressed the activation of respiration by exogenous ADP, failed to abolish the stimulation of respiration by endogenous ADP, but did inhibit respiration after the cells were treated with trypsin. The addition of up to 4 μM of free Ca2+ to the actively respiring fibres resulted in reversible hypercontraction associated with a decreased apparent Km for exogenous ADP. These changes were fully abolished in fibres after the removal of myosin by KCl treatment.CONCLUSIONS:
Mitochondria and ATPases, together with cytoskeletal proteins that establish the structural links between mitochondria and sarcomeres, form complexes – intracellular energetic units (ICEUs) – in cardiac cells. Within the ICEUs, the mitochondria and ATPases interact via specialized energy transfer systems, such as the creatine kinase- and adenylate kinase-phosphotransfer networks, and direct ATP channelling. Disintegration of the structure and function of ICEUs results in dyscompartmentation of adenine nucleotides and may represent a basis for cardiac diseases. 相似文献26.
Wolfram Pönisch Bruno Holzvogt Madlen Plötze Marc Andrea Malvina Bourgeois Simone Heyn Thomas Zehrfeld Doreen Hammerschmidt Maik Schwarz Thomas Edelmann Cornelia Becker Franz Albert Hoffmann Andreas Schwarzer Ute Kreibich Kerstin Gutsche Kolja Reifenrath Cornelia Winkelmann Rainer Krahl Yvonne Remane Evelin Hennig Thomas Schliwa Tom Lindner Thorsten Kaiser Vladan Vucinic Gerhard Behre Dietger Niederwieser 《Journal of cancer research and clinical oncology》2014,140(11):1947-1956
Introduction
Bortezomib is a proteasome inhibitor that has shown important clinical efficacy either as a single agent or in combination in patients with multiple myeloma (MM). In the present protocol, bortezomib was combined with bendamustine and prednisone, in order to assess the efficacy and safety of this combination therapy in patients with newly diagnosed/untreated MM.Methods
Between June 2006 and October 2013, 49 patients with newly diagnosed/untreated MM were treated with bendamustine 60 mg/m2 on days 1 and 2, bortezomib 1.3 mg/m2 on days 1, 4, 8 and 11, and prednisone 100 mg on days 1, 2, 4, 8 and 11 bendamustine, prednisone and bortezomib (BPV) once every 21 days. Patients were divided into three groups: group A (n = 19) consisted of patients with normal renal function or mild dysfunction (eGFR ≥60 ml/min), group B (n = 15) patients with moderate or severe renal dysfunction (eGFR 15–59 ml/min) and group C (n = 15) patients with renal failure/dialysis (eGFR <15 ml/min).Results
A median number of two (range 1–5) BPV treatment cycles were given to the patients. The majority of the patients (n = 40, 82 %) responded after at least one cycle of BPV therapy with five stringent complete responses (CRs), nine near complete responses, 12 very good partial responses and 14 partial responses. Five patients had MR, three stable and one progressive disease. After a median observation time of 13 months, progression-free survival (PFS) and overall survival (OS) at 12 months were 92 and 94 %, respectively, for patients with normal renal function or mild renal dysfunction (group A) and 83 and 93 %, respectively, for patients with moderate or severe renal dysfunction (group B). Outcome for these patients was slightly better but not statistically significantly better than that for patients with renal failure/dialysis (group C), who had a PFS, and OS of 66 % (p = 0.08) and 73 % (p = 0.05), respectively.Summary
These results indicate that this BPV combination is feasible, effective and well tolerated in patients with newly diagnosed MM and normal or impaired renal function. 相似文献27.
28.
Amy D. Nguyen Katy Slack Christoph Schwarzer Nicola J. Lee Dana Boey Laurence Macia Ernie Yulyaningsih Ronaldo F. Enriquez Lei Zhang Shu Lin Yan-Chuan Shi Paul A. Baldock Herbert Herzog Amanda Sainsbury 《Neuropeptides》2014
Objective
Orexigenic neuropeptide Y (NPY) and dynorphin (DYN) regulate energy homeostasis. Single NPY or dynorphin deletion reduces food intake or increases fat loss. Future developments of obesity therapeutics involve targeting multiple pathways. We hypothesised that NPY and dynorphin regulate energy homeostasis independently, thus double NPY and dynorphin ablation would result in greater weight and/or fat loss than the absence of NPY or dynorphin alone.Design and methods
We generated single and double NPY and dynorphin knockout mice (NPYΔ, DYNΔ, NPYDYNΔ) and compared body weight, adiposity, feeding behaviour, glucose homeostasis and brown adipose tissue uncoupling protein-1 (UCP-1) expression to wildtype counterparts.Results
Body weight and adiposity were significantly increased in NPYDYNΔ, but not in NPYΔ or DYNΔ. This was not due to increased food intake or altered UCP-1 expression, which were not significantly altered in double knockouts. NPYDYNΔ mice demonstrated increased body weight loss after a 24-h fast, with no effect on serum glucose levels after glucose injection.Conclusions
Contrary to the predicted phenotype delineated from single knockouts, double NPY and dynorphin deletion resulted in heavier mice, with increased adiposity, despite no significant changes in food intake or UCP-1 activity. This indicates that combining long-term opioid antagonism with blockade of NPY-ergic systems may not produce anti-obesity effects. 相似文献29.
Wagner F. Gattaz Leda L. Talib Evelin L. Schaeffer Breno S. Diniz Orestes V. Forlenza 《Journal of neural transmission (Vienna, Austria : 1996)》2014,121(2):193-200
Reduced phospholipase A2 (PLA2) activity has been reported in the brain and in blood cells of patients with Alzheimer’s disease (AD). Individuals with mild cognitive impairment (MCI) are at increased risk of developing AD. In the present study, we determined the activity of distinct PLA2 subgroups (iPLA2, sPLA2 and cPLA2) in older adults with MCI as compared to patients with mild dementia due to AD and to cognitively healthy controls. We investigated whether decreased PLA2 activity at baseline is associated with the progression of MCI to AD upon follow-up during a period of 4 years. The activity of PLA2 subgroups was determined in platelets from 169 elderly adults. Progression of MCI to AD was ascertained by standard clinical criteria for AD upon follow-up. At baseline, iPLA2 activity was significantly decreased (p = 0.001) in patients with AD and MCI as compared to controls. Patients with MCI who progressed to AD during follow-up showed significantly lower iPLA2 activity at baseline as compared to patients with MCI who did not progress to AD (p = 0.009). Moreover, subjects from the control group at baseline who progressed to MCI during follow-up had lower sPLA2 and cPLA2 (p = 0.014 and p = 0.009, respectively). Our findings suggest that low platelet iPLA2 activity may be a risk marker for AD in subjects with MCI. Moreover, low sPLA2 and cPLA2 were related to cognitive decline in healthy controls, suggesting a relationship with the very early stages of the disease. 相似文献
30.
Prostate adenocarcinoma (PCa) stromal markers have recently gained attention as complementary diagnostic tools. The DNA reparation complex protein FANCM has been shown to express in the normal prostate stroma and FANCM gene alterations to be associated with PCa susceptibility; this has led to the hypothesis that an insufficient level of FANCM expression may provide additional information for the evaluation of PCa. The study cohort comprised 60 radical prostatectomy specimens. The controls involved 11 autopsies (CTRL) and non‐cancerous tissue (NCT) areas from the prostatectomy specimen. The samples were stained with the FANCM antibody. The quantification of the stromal staining index (SSI) was made using ImageJ and QuPath. Overall, 655 regions of interest (ROI) were analyzed. FANCM expression appeared equally intense and stroma specific in both CTRL and NCT, indicating the absence of underlying baseline alterations. Within the age span of the cohort 47–89 years, no significant effect of the age of the patients on the FANCM expression was seen. FANCM demonstrated Gleason grade (G) dependent decline in PCa, being statistically significant in controls versus G1 and G2 versus G3. In other adjacent International Society of Urological Pathology (ISUP) groups, it remained insignificant, still being meaningful between high and low‐grade cancers. 相似文献