全文获取类型
收费全文 | 497篇 |
免费 | 102篇 |
国内免费 | 7篇 |
专业分类
耳鼻咽喉 | 1篇 |
儿科学 | 12篇 |
妇产科学 | 5篇 |
基础医学 | 58篇 |
口腔科学 | 3篇 |
临床医学 | 83篇 |
内科学 | 70篇 |
皮肤病学 | 12篇 |
神经病学 | 12篇 |
特种医学 | 122篇 |
外科学 | 23篇 |
综合类 | 36篇 |
预防医学 | 23篇 |
眼科学 | 1篇 |
药学 | 9篇 |
中国医学 | 2篇 |
肿瘤学 | 134篇 |
出版年
2024年 | 2篇 |
2023年 | 2篇 |
2022年 | 5篇 |
2021年 | 9篇 |
2020年 | 8篇 |
2019年 | 10篇 |
2018年 | 26篇 |
2017年 | 13篇 |
2016年 | 23篇 |
2015年 | 14篇 |
2014年 | 24篇 |
2013年 | 32篇 |
2012年 | 16篇 |
2011年 | 20篇 |
2010年 | 19篇 |
2009年 | 25篇 |
2008年 | 17篇 |
2007年 | 18篇 |
2006年 | 9篇 |
2005年 | 10篇 |
2004年 | 24篇 |
2003年 | 25篇 |
2002年 | 30篇 |
2001年 | 23篇 |
2000年 | 13篇 |
1999年 | 16篇 |
1998年 | 31篇 |
1997年 | 14篇 |
1996年 | 13篇 |
1995年 | 12篇 |
1994年 | 15篇 |
1993年 | 16篇 |
1992年 | 4篇 |
1991年 | 3篇 |
1989年 | 8篇 |
1988年 | 7篇 |
1987年 | 4篇 |
1986年 | 7篇 |
1985年 | 4篇 |
1984年 | 3篇 |
1983年 | 5篇 |
1982年 | 6篇 |
1981年 | 2篇 |
1980年 | 4篇 |
1979年 | 2篇 |
1978年 | 5篇 |
1977年 | 2篇 |
1976年 | 1篇 |
1975年 | 3篇 |
1974年 | 1篇 |
排序方式: 共有606条查询结果,搜索用时 37 毫秒
41.
Modern surgical teaching is evolving at speed. The environment in which we learn and teach is subject to a number of extrinsic pressures. These are influencing ways in which medical students and trainees learn and are taught the discipline of surgery. As a surgical educator it is useful to understand the reasons for these changes and to acquire the skills necessary to provide effective teaching. This short article looks at reasons why one would wish to develop surgical teaching skills, covers basic principles associated with delivery of effective teaching sessions and briefly reviews other avenues that may enhance teaching practice. 相似文献
42.
43.
CT Thompson 《Journal of cutaneous pathology》2005,32(1):118-118
Large cell transformation of mycosis fungoides (MF) is an uncommon phenomenon. We present a case of CD30‐positive large cell transformation and discuss its possible pathophysiology. A 74 year‐old male with a 36‐year history of patch stage MF presented with a 3‐month history of right chest cellulitis that was refractory to IV antibiotic treatment. Skin biopsies from his thigh demonstrated a patchy dermal infiltrate of irregular and hyperchromatic lymphocytes and epidermotropism. The majority of the infiltrate was positive for CD4, CD3, CD2, and negative for CD7. Only 10% were positive for CD25 and CD8. Biopsies obtained from the ulcerated chest nodules showed a dermal infiltrate of large and pleomorphic lymphoid cells with prominent nucleoli. These large lymphoid cells were strongly positive for CD3, CD30, CD25, CD2 and UCHL‐1. Occasional cells were positive for CD4 and CD20. They were negative for ALK‐1, TIA‐1, CD7, CD8, and CD15. T‐gamma receptor gene rearrangement analyses by polymerase chain reaction demonstrated a clonal process with similar rearrangement patterns identified in the patch stage MF as well as in large cell transformation areas. Examinations of his peripheral blood and bone marrow were negative. The patient had tolerated one cycle of CHOP chemotherapy. 相似文献
44.
Fadi Brimo MD Andrew A. Renshaw MD Majorie Deschenes MD Michele Charbonneau CT Manon Auger MD 《Cancer cytopathology》2009,117(5):311-317
BACKGROUND:
Documenting the performance of gynecologic screening in actual practice settings is difficult to achieve. In the current study, the screening performance of 11 individual cytotechnologists as well as that of the overall laboratory over 2 consecutive time periods was examined using the rapid prescreening (RPS) method.METHODS:
RPS was performed by all cytotechnologists in a single laboratory over 2 separate 8‐month periods. The sensitivity of screening for individual and groups of cytotechnologists was examined. For purposes of comparison, cytotechnologists were divided into 2 groups: screeners with an overall routine sensitivity ≥95% and screeners with an overall sensitivity <95%.RESULTS:
Atypical squamous cells (ASC) were used as a threshold, and routine screening sensitivity was found to vary from 68.3% to 96.8%. The overall sensitivity of the laboratory for RPS and routine screening was 43.6% and 88.4%, respectively. Over time, the overall laboratory sensitivity of routine screening improved from 85.3% to 91.3% (P = .01). During this same time frame, the sensitivity of the screeners with an overall sensitivity <95% improved from 79.3% to 91.2% (P < .001), whereas the sensitivity of screeners with an overall routine sensitivity ≥95% remained the same (96.1% to 96.4%; P = .6).CONCLUSIONS:
In addition to improved overall performance of the laboratory by detecting and correcting errors, the results of the current study indicate that using RPS consistently over time might play a role leading to improved performance of cytotechnologists with an overall routine sensitivity <95% but not of cytotechnologists with an overall routine sensitivity ≥95%. Cancer (Cancer Cytopathol) 2009. © 2009 American Cancer Society. 相似文献45.
46.
Slingsby JH Baban D Sutton J Esapa M Price T Kingsman SM Kingsman AJ Slade A 《Human gene therapy》2000,11(10):1439-1451
A number of stable producer cell lines for high-titer Mo-MuLV vectors have been constructed. Development has previously centered on increasing end-point titers by producing maximal levels of Mo-MuLV Gag/Pol, envelope glycoproteins, and retroviral RNA genomes. We describe the production yields and transduction efficiency characteristics of two Mo-MuLV packaging cell lines, FLYA13 and TEFLYA. Although they both produce 4070A-pseudotyped retroviral vectors reproducibly at >1 x 10(6) LFU ml(-1), the transduction efficiency of unconcentrated and concentrated virus from FLYA13 lines is poor compared with vector preparations from TEFLYA lines. A powerful inhibitor of retroviral transduction is secreted by FLYA13 packaging cells. We show that the inhibitory factor does not affect transduction of target cells by RD114-pseudotyped vectors. This suggests that the inhibitory factor functions at the level of envelope-receptor interactions. Phosphate starvation of target cells shows a two-fold increase in Pit2 receptor mRNA and causes some improvement in FLYA13 virus transduction efficiency. Western blots show that FLYA13 viral samples contain an eight-fold higher ratio of 4070A envelope to p30gag than that of virus produced by TEFLYA producer cell lines. This study correlates overexpression of 4070A envelope glycoprotein in retroviral preparations with a reduction of transduction efficiency at high multiplicities of infection. We suggest that TEFLYA packaging cells express preferable levels of 4070A compared with FLYA13, which not only enables high-titer stocks to be generated, but also facilitates a high efficiency of transduction of target cells. 相似文献
47.
Negative Pap tests in women with high‐grade cervical lesions on follow‐up biopsies: Contributing factors and role of human papillomavirus genotyping
下载免费PDF全文
![点击此处可从《Diagnostic cytopathology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
48.
Naoto Kuroda MD Hiroyuki Tamiya MD Kimiko Nakatani MD Haruna Ide MS Yukari Wada CT Kaori Yasuoka CT Masahiko Ohara CT Keiko Mizuno CT Kenji Yorita MD Kengo Takeuchi MD 《Diagnostic cytopathology》2018,46(4):336-339
ROS1‐rearranged lung adenocarcinoma has been recently identified. We report a case of ROS1‐rearranged lung adenocarcinoma with special emphasis on cytological findings. Here, we report a case of young woman with ROS1‐rearranged lung adenocarcinoma diagnosed by cytology and discuss the clinical, cytological, and molecular findings. Cytologically, the tumor consisted of small tight clusters of cells with high nuclear/cytoplasmic ratio. Nuclei were enlarged and small nucleoli were occasionally observed. Signet‐ring cells were focally identified. Neoplastic cells were positive for ROS1 immunocytochemistry. Subsequently, the translocation of ROS1 gene was confirmed in a histological specimen. In conclusion, the specific histology of adenocarcinoma on cytological materials should promote testing for ROS1 immunohistochemistry. Immunocytochemical detection of ROS1 protein helps identify patients suitable for molecular targeted therapy. 相似文献
49.
Useful aspects of diagnosis of imprint cytology in intraoperative consultation of ovarian tumors: comparison between imprint cytology and frozen sections
下载免费PDF全文
![点击此处可从《Diagnostic cytopathology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
50.