全文获取类型
收费全文 | 272篇 |
免费 | 19篇 |
专业分类
耳鼻咽喉 | 1篇 |
儿科学 | 8篇 |
妇产科学 | 1篇 |
基础医学 | 42篇 |
临床医学 | 25篇 |
内科学 | 65篇 |
神经病学 | 8篇 |
特种医学 | 51篇 |
外科学 | 32篇 |
综合类 | 1篇 |
预防医学 | 18篇 |
眼科学 | 1篇 |
药学 | 15篇 |
肿瘤学 | 23篇 |
出版年
2023年 | 6篇 |
2022年 | 18篇 |
2021年 | 30篇 |
2020年 | 7篇 |
2019年 | 16篇 |
2018年 | 8篇 |
2017年 | 5篇 |
2016年 | 14篇 |
2015年 | 14篇 |
2014年 | 15篇 |
2013年 | 11篇 |
2012年 | 27篇 |
2011年 | 24篇 |
2010年 | 14篇 |
2009年 | 5篇 |
2008年 | 13篇 |
2007年 | 9篇 |
2006年 | 12篇 |
2005年 | 4篇 |
2004年 | 9篇 |
2003年 | 11篇 |
2002年 | 6篇 |
2001年 | 4篇 |
2000年 | 2篇 |
1999年 | 1篇 |
1997年 | 2篇 |
1996年 | 1篇 |
1994年 | 1篇 |
1992年 | 1篇 |
1982年 | 1篇 |
排序方式: 共有291条查询结果,搜索用时 15 毫秒
21.
Acetylation of mitogen-activated protein kinase phosphatase-1 inhibits Toll-like receptor signaling 总被引:1,自引:0,他引:1
The mitogen-activated protein kinase (MAPK) pathway plays a critical role in Toll-like receptor (TLR) signaling. MAPK phosphatase-1 (MKP-1) inhibits the MAPK pathway and decreases TLR signaling, but the regulation of MKP-1 is not completely understood. We now show that MKP-1 is acetylated, and that acetylation regulates its ability to interact with its substrates and deactivate inflammatory signaling. We found that LPS activates acetylation of MKP-1. MKP-1 is acetylated by p300 on lysine residue K57 within its substrate-binding domain. Acetylation of MKP-1 enhances its interaction with p38, thereby increasing its phosphatase activity and interrupting MAPK signaling. Inhibition of deacetylases increases MKP-1 acetylation and blocks MAPK signaling in wild-type (WT) cells; however, deacetylase inhibitors have no effect in cells lacking MKP-1. Furthermore, histone deacetylase inhibitors reduce inflammation and mortality in WT mice treated with LPS, but fail to protect MKP-1 knockout mice. Our data suggest that acetylation of MKP-1 inhibits innate immune signaling. This pathway may be an important therapeutic target in the treatment of inflammatory diseases. 相似文献
22.
23.
Elizaveta Chabanova Ingegerd BalslevVibeke Logager Alastair HansenHenrik Jakobsen Bjarne Kromann-AndersenNis Norgaard Thomas HornHenrik S. Thomsen 《European journal of radiology》2011,80(2):292-296
Purpose
To investigate diagnostic accuracy of detection of prostate cancer by magnetic resonance: to evaluate the performance of T2WI, DCEMRI and CSI and to correlate the results with biopsy and radical prostatectomy histopathological data.Materials and methods
43 patients, scheduled for radical prostatectomy, underwent prostate MR examination. Prostate cancer was identified by transrectal ultrasonographically (TRUS) guided sextant biopsy. MR examination was performed at 1.5T with an endorectal MR coil. Cancer localisation was performed on sextant-basis - for comparison between TRUS biopsy, MR techniques and histopathological findings on prostatectomy specimens.Results
Prostate cancer was identified in all 43 patients by combination of the three MR techniques. The detection of prostate cancer on sextant-basis showed sensitivity and specificity: 50% and 91% for TRUS, 72% and 55% for T2WI, 49% and 69% for DCEMRI, and 46% and 78% for CSI.Conclusion
T2WI, DCEMRI and CSI in combination can identify prostate cancer. Further development of MR technologies for these MR methods is necessary to improve the detection of the prostate cancer. 相似文献24.
Giuseppe Filardo Elizaveta Kon Roberto Buda Antonio Timoncini Alessandro Di Martino Annarita Cenacchi Pier Maria Fornasari Sandro Giannini Maurilio Marcacci 《Knee surgery, sports traumatology, arthroscopy》2011,19(4):528-535
Purpose
Platelet-rich plasma (PRP) therapy is a simple, low-cost and minimally invasive method that provides a natural concentrate of autologous blood growth factors (GFs) that can be used to enhance tissue regeneration. In a previous analysis of a 12-month follow-up study, promising results were obtained when treating patients affected by knee degeneration with PRP intra-articular injections. The main purpose of this study was to investigate the persistence of the beneficial effects observed. 相似文献25.
Stem cells associated with macroporous bioceramics for long bone repair: 6- to 7-year outcome of a pilot clinical study 总被引:4,自引:0,他引:4
Marcacci M Kon E Moukhachev V Lavroukov A Kutepov S Quarto R Mastrogiacomo M Cancedda R 《Tissue engineering》2007,13(5):947-955
Extensive bone loss is still a major problem in orthopedics. A number of different therapeutic approaches have been developed and proposed, but so far none have proven to be fully satisfactory. We used a new tissue engineering approach to treat four patients with large bone diaphysis defects and poor therapeutic alternatives. To obtain implantable three-dimensional (3D) living constructs, cells isolated from the patients' bone marrow stroma were expanded in culture and seeded onto porous hydroxyapatite (HA) ceramic scaffolds designed to match the bone deficit in terms of size and shape. During the surgical session, an Ilizarov apparatus or a monoaxial external fixator was positioned on the patient's affected limb and the ceramic cylinder seeded with cells was placed in the bone defect. Patients were evaluated at different postsurgery time intervals by conventional radiographs and computed tomography (CT) scans. In one patient, an angiographic evaluation was performed at 6.5 years follow-up. In this study we analyze the long-term outcome of these patients following therapy. No major complications occurred in the early or late postoperative periods, nor were major complaints reported by the patients. No signs of pain, swelling, or infection were observed at the implantation site. Complete fusion between the implant and the host bone occurred 5 to 7 months after surgery. In all patients at the last follow-up (6 to 7 years postsurgery in patients 1 to 3), a good integration of the implants was maintained. No late fractures in the implant zone were observed. The present study shows the long-term durability of bone regeneration achieved by a bone engineering approach. We consider the obtained results very promising and propose the use of culture-expanded osteoprogenitor cells in conjunction with porous bioceramics as a real and significant improvement in the repair of critical-sized long bone defects. 相似文献
26.
Pawe Holewa Jakub Jasiski Artem Shikin Elizaveta Lebedkina Aleksander Maryski Marcin Syperek Elizaveta Semenova 《Materials》2021,14(2)
The InAs/InP quantum dots (QDs) are investigated by time-integrated (PL) and time-resolved photoluminescence (TRPL) experiments. The QDs are fabricated site-selectively by droplet epitaxy technique using block copolymer lithography. The estimated QDs surface density is ∼1.5 × 1010 cm−2. The PL emission at is centered at 1.5 μm. Below , the PL spectrum shows a fine structure consisting of emission modes attributed to the multimodal QDs size distribution. Temperature-dependent PL reveals negligible carrier transfer among QDs, suggesting good carrier confinement confirmed by theoretical calculations and the TRPL experiment. The PL intensity quench and related energies imply the presence of carrier losses among InP barrier states before carrier capture by QD states. The TRPL experiment highlighted the role of the carrier reservoir in InP. The elongation of PL rise time with temperature imply inefficient carrier capture from the reservoir to QDs. The TRPL experiment at reveals the existence of two PL decay components with strong dispersion across the emission spectrum. The decay times dispersion is attributed to different electron-hole confinement regimes for the studied QDs within their broad distribution affected by the size and chemical content inhomogeneities. 相似文献
27.
28.
Bernhard F. Gibbs Isabel Gon?alves Silva Alexandr Prokhorov Maryam Abooali Inna M. Yasinska Maxwell A. Casely-Hayford Steffen M. Berger Elizaveta Fasler-Kan Vadim V. Sumbayev 《Oncotarget》2015,6(30):28678-28692
Correction of human myeloid cell function is crucial for the prevention of inflammatory and allergic reactions as well as leukaemia progression. Caffeine, a naturally occurring food component, is known to display anti-inflammatory effects which have previously been ascribed largely to its inhibitory actions on phosphodiesterase. However, more recent studies suggest an additional role in affecting the activity of the mammalian target of rapamycin (mTOR), a master regulator of myeloid cell translational pathways, although detailed molecular events underlying its mode of action have not been elucidated. Here, we report the cellular uptake of caffeine, without metabolisation, by healthy and malignant hematopoietic myeloid cells including monocytes, basophils and primary acute myeloid leukaemia mononuclear blasts. Unmodified caffeine downregulated mTOR signalling, which affected glycolysis and the release of pro-inflammatory/pro-angiogenic cytokines as well as other inflammatory mediators. In monocytes, the effects of caffeine were potentiated by its ability to inhibit xanthine oxidase, an enzyme which plays a central role in human purine catabolism by generating uric acid. In basophils, caffeine also increased intracellular cyclic adenosine monophosphate (cAMP) levels which further enhanced its inhibitory action on mTOR. These results demonstrate an important mode of pharmacological action of caffeine with potentially wide-ranging therapeutic impact for treating non-infectious disorders of the human immune system, where it could be applied directly to inflammatory cells. 相似文献
29.
Luyten FP Denti M Filardo G Kon E Engebretsen L 《Knee surgery, sports traumatology, arthroscopy》2012,20(3):401-406
With the emerging interest in regenerative medicine and tissue engineering, new treatment modalities being developed for joint
disorders including joint surface lesions and articular cartilage defects. The clinical outcome of these novel approaches
appears rather unpredictable and is due to many reasons but definitely also linked to the patient profile. As a typical example,
symptomatic articular cartilage lesions can be presented in an otherwise normal joint, or associated with several other joint
tissue alterations including meniscal lesions and abnormalities of the underlying bone. The outcome of novel treatments may
well be influenced by the status of the whole joint, and the potential to develop osteoarthritis. To better identify the patients
at risk and responders to certain treatments, it is of use to define and most importantly classify patients with “early osteoarthritis”.
Here, classification criteria for this group of patients are presented, allowing a more defined and accurate inclusion in
clinical trials in the future. 相似文献
30.
Filardo G Kon E Di Martino A Patella S Altadonna G Balboni F Bragonzoni L Visani A Marcacci M 《Knee surgery, sports traumatology, arthroscopy》2012,20(9):1704-1713