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51.
Foncuberta MC Cagnoni PJ Brandts CH Mandanas R Fields K Derigs HG Reed E Sonis ST Fay J LeVeque F Pouillart P Schrezenmeier H Emmons R Thiel E;Investigators in TGF-beta/OM Study Protocols / 《Journal of immunotherapy (Hagerstown, Md. : 1997)》2001,24(4):384-388
Transforming growth factor (TGF)-beta3 has been hypothesized to prevent or alleviate oral mucositis (OM) in cancer patients receiving high-dose chemotherapy (CT). Two double-blind, placebo-controlled, multicenter, phase II studies of TGF-beta3 were initiated in the United States, Europe, and Argentina in patients with lymphomas or solid tumors who were receiving highly stomatotoxic CT regimens. Patients were to apply 10-mL mouthwash applications of TGF-beta3 (25 microg/mL) or placebo four times daily (or twice daily) 1 day before and all days during CT. The patients were subsequently evaluated for OM incidence, severity, and duration using National Institute of Cancer Common Toxicity Criteria (NCI-CTC) criteria and an objective scoring system (1). After the start of the trials, negative results from new preclinical studies suggesting suboptimal formulation and/or dosing led to an interim analysis of the ongoing clinical trials. One hundred fifty-two patients from the combined studies were included in the interim analysis, with 116 patients on the TGF-beta3 four times daily and placebo arms. Most (72%) patients had breast cancer, 22% had lymphomas, and 6% had other solid tumors. Although 98% (149 of 152) of patients experienced adverse events, only 14% (22 of 152) experienced events that were judged as possibly or probably related to the study drug (primarily gastrointestinal symptoms). No clinically relevant differences were seen between the treatment and placebo arms regarding safety, nor was there evidence for systemic absorption of TGF-beta3. Finally, there was no advantage of TGF-beta3 treatment regarding the incidence (TGF-beta3 four times daily versus placebo [46% versus 47%]), onset, or duration of NCI-CTC grade 3 or 4 OM. For this dose, formulation, regimen. and patient population, TGF-beta3 was not effective in the prevention or alleviation of CT-induced OM. 相似文献
52.
Seventy-five spot compression views of equivocally suspicious lesions detected at routine mammographic examination of 72 women were reviewed in this retrospective study. Sixty-five of the 75 lesions appeared less suspicious on spot compression views, two did not change, and eight appeared more suspicious. Biopsy findings confirmed that the eight more suspicious lesions were cancer. The adjunctive use of spot compression helped characterize equivocal findings seen on routine mammographic views and improved the accuracy of mammographic interpretation. 相似文献
53.
Immobilizers are described that have been used during computed tomography (CT) to ensure reproducibility of patient position during all phases of radiation treatment planning and delivery. The immobilizer causes no degradation or artifact on CT images; hence, the value of the images in radiation treatment planning is not compromised. 相似文献
54.
S.K. GUPTA OM SINGH G.P. TALWAR 《American journal of reproductive immunology (New York, N.Y. : 1989)》1985,7(3):104-108
ABSTRACT: Mouse hybrid cell clones secreting antigonadotropin releasing hormone monoclonal antibody were developed by fusion of SP2/O-Ag 1.4 myeloma cells with splenocytes of mouse immunized with gonadotropin releasing hormone (GnRH) tagged to tetanus toxoid. The product of hybrid cell clones obtained as ascites fluid from mouse peritoneal cavity had a titre of 106 (30–40% binding of 125I-GnRH) in radioimmunoassay. The antibody was IgG2a and Kappa. The association constant (Ka) of the product of hybrid cell clone P81662 for binding with GnRH was 1.2 × 109 L/mole. The monoclonal antibody (P81662) was highly specific for the native GnRH and devoid of reactivity with thyroid releasing hormone as tested in competitive radioimmunoassay. The recognition for GnRH agonists by monoclonal was 387-fold less with D-Ser (But)6 des Gly10 GnRH ethylamide and 608-fold less with Bz1-His6 GnRH. Monoclonal anti-GnRH antibody was competent to neutralize the in vivo bioactivity of the hormone as evident by the block of estrus cycle and termination of pregnancy in mice. Termination of pregnancy in animals receiving anti-GnRH monoclonal could be prevented by administration of progesterone. 相似文献
55.
56.
A simplified serum-free method for preparation and cultivation of human granulosa-luteal cells 总被引:1,自引:0,他引:1
Figenschau Y; Sundsfjord JA; Yousef MI; Fuskevag OM; Sveinbjornsson B; Bertheussen K 《Human reproduction (Oxford, England)》1997,12(3):523-531
A simplified method for the preparation and long-term cultivation of
granulosa-luteal cells in serum-free medium is described. The cells were
harvested from women undergoing in-vitro fertilization, enriched by
sedimentation and dissociated by enzymatic treatment. We demonstrated, by
introducing a synthetic serum replacement (SSR2), that these primary cell
cultures cultivated in monolayers on an extracellular matrix may be used in
experiments exceeding 7 days with low cell loss and cell death. No adverse
effect on progesterone production was found. There was a high diversity in
progesterone production between cells from individual patients. After
several days in culture, the cells were challenged with human chorionic
gonadotrophin which revived the rapidly decreasing progesterone production.
We were unable to demonstrate an increase in cell number after 7 days of
cultivation when the cells were grown in medium supplemented with either
serum or SSR2. The mitogens epidermal growth factor and basic fibroblast
growth factor had no influence on proliferation. We also found that the
present method prevents leukocyte contamination in the granulosa-luteal
cell cultures. Compared with the common method based on the enrichment of
granulosa-luteal cells on a density gradient (Ficoll/Percoll), this method
saves time, labour and expense, in addition to augmenting purity.
相似文献
57.
Is the male involved in the aetiology of ectopic pregnancy? 总被引:3,自引:0,他引:3
Warnes GM; Petrucco OM; Seamark RF; Lancaster PA 《Human reproduction (Oxford, England)》1998,13(12):3505-3510
We have previously observed a low incidence of ectopic pregnancies in
couples having gamete intra-Fallopian transfer (GIFT) with donated
spermatozoa. Based on findings in both animal and human models, we proposed
the hypothesis that sperm defects may be associated with the expression of
paternal genes which cause abnormal early embryo development and predispose
the embryos to interact inappropriately with the genital tract epithelium,
and so increase the risk of an ectopic implantation. To both confirm and
extend the initial observation, GIFT and in-vitro fertilization (IVF)
pregnancies entered on the Australian and New Zealand national database
between 1979 and 1993 were analysed with regard to the incidence of ectopic
pregnancy. There was an increased risk of ectopic pregnancy for IVF
relative to GIFT and when spermatozoa from the male partner were used
rather than donor spermatozoa. However, when couples were categorized with
respect to the aetiology of their infertility, we were unable to show a
significant association between ectopic pregnancy and whether spermatozoa
from the male partner or a donor were used. We have therefore been unable
to confirm a direct association between the source of spermatozoa and
ectopic pregnancy.
相似文献
58.
Antibody-induced antigenic modulation (AIAM) of CD10 and CD19 was studied on NALM-6, RAJI, and JOK-1 cell lines using fluorescence microscopy (FM), flow cytometry (FCM), and immunoelectron microscopy (IEM). Cross-linking with monoclonal antibodies (MoAbs) induced rapid redistribution of CD10 and CD19 on the cell surface (FM) followed by internalization involving uptake through plasmalemmal pits, transfer through endosomal compartment (receptor-mediated endocytosis), and, finally, delivery to lysosomes for degradation or exocytosis and recycling (IEM). Significant quantitative differences regarding modulation and intracellular processing were shown by FCM and IEM. Thus, 35%, 30%, and 25% of CD10 compared with 80%, 60%, and 40% of CD19 were internalized in NALM-6, RAJI, and JOK-1 cells, respectively. Also, the rate of intracellular transfer as well as externalization and recycling was more pronounced in the case of CD19 than of CD10 and in the NALM-6 and RAJI cells compared with the JOK-1 cells. These differences may possibly reflect the functional significance of CD10 and CD19 as well as the stage of differentiation of the malignant B cells. Although both antigens can be useful in MoAb-targeted immunotherapy, our findings suggest that anti-CD19 MoAbs would be preferable for delivery of cytotoxic agents to malignant B cells. 相似文献
59.
Objective
To evaluate antidiabetic and hypolipidemic activities of Kigelia pinnata methanolic flowers extract in streptozotocin (STZ) induced diabetic wistar rat.Methods
Rats were made diabetic by a single dose of STZ at 60 mg/kg body weight i.p. The blood glucose level was checked before and 72 h after STZ injection to confirm the development of diabetes. The flower extract and glibenclamide were administered orally at the doses of 250 and 500 mg/kg body weight for 21 days.Results
Daily oral treatment with the extract and standard drug for 21 days significantly reduced blood glucose, serum cholesterol and triglycerides levels. High density lipoprotein-cholesterol level was found to be improved (P<0.01) as compared to diabetic control group.Conclusions
It is concluded that Kigellia pinnata flowers extract have significant antidiabetic and hypolipidemic effect. 相似文献60.