胃癌病人的白细胞介素6水平检测及其临床意义

[目的 ]检测胃癌病人血清白细胞介素 6 (interleukin 6 ,IL 6 )的动态变化 ,了解IL 6含量与胃癌的相关性 .[方法 ]采用酶联免疫吸附测定法 (双抗体夹心法 )动态检测 4 7例胃癌病人手术前后血清IL 6值的变化 ,并与正常人进行对比及相关分析 .[结果 ]胃癌病人血清IL 6水平明显高于正常人 ,而病人术后血清IL 6水平低于术前水平 .[结论 ]胃癌病人血清IL 6水平检测可作为评价胃癌病人的病情与疗效的辅助指标     

Clinical Features of Oxaliplatin-induced Hypersensitivity Reactions in Chinese Patients:A Retrospective Multicenter Analysis

Objective:The characteristics of oxaliplatin-induced hypersensitivity reactions(HSRs)in Chinese patients were investigated to provide a reference for patients t...     

A pilot investigation of e‐cigarette use and smoking behaviour among patients with chronic airway disease or respiratory symptoms

BackgroundThis pilot study aimed to investigate the current status of e‐cigarettes (ECs) use patterns among patients with chronic airway disease or chronic respiratory symptoms and the effects of ECs use on respiratory and mental health.MethodsA cross‐sectional survey was conducted at the outpatient clinic of eight teaching hospitals in South Korea between November 2019 and December 2019. All adult ECs users (19 years and above) who visited the outpatient clinic as a patient with chronic airway disease or chronic respiratory symptoms were eligible to participate in this study.ResultsA total of 51 subjects responded to the survey. Most of the participants were male (92.2%) and the mean age was 41.8 years. Dominant airway diseases were asthma and chronic obstructive pulmonary disease. Most of the subjects had a history of cigarette smoking, and 19 subjects were dual users of current cigarettes and ECs. Most of the subjects started ECs use due to health‐related reasons. When comparing exclusive ECs users and dual users, St. George''s respiratory questionnaire (SGRQ) scores, the proportion of cases with moderate to severe depressive symptoms, and average Fagerstrom test for nicotine dependence scores for ECs were higher in dual users than exclusive ECs users (mean 4.64 vs. 2.38, p = 0.006), respectively.ConclusionMost of the subjects started ECs use due to health concerns, but dual users have more respiratory symptoms and higher nicotine dependence in this pilot study. One hypothesis that comes from these results is that greater nicotine dependence may influence behaviours, habits, and views about ECs. These preliminary observations need confirmation in a large cohort.     

实施院内设备共用或借用管理的体会

为了提高设备使用率,对部分抢救治疗设备实施共用或借用管理的尝试和体会。     

食管癌、贲门癌术后呼吸功能不全高危因素分析

目的:探讨食管癌和贲门癌术后发生呼吸功能不全的高危因素。方法:采用回顾性病例对照研究,应用Logistic回归分析食管癌和贲门癌术后呼吸功能不全发生的高危因素。结果:经Logistic回归分析,术后发生呼吸功能不全的高危因素按相关强度依次为:术前合并COPD、手术时间长、高龄、手术日输液量多。结论:术前合并COPD(chronic obstructive pulmonary disease)、手术时间长、高龄是食管癌术后发生呼吸功能不全的高危因素;严格手术适应证,术者的丰富经验及娴熟技术,积极围手术期处理是防治术后呼吸功能不全的有效措施。     

眼眶瘘管形成的病因及诊疗分析

目的 探讨眼眶瘘管形成的原因,提高其诊治水平.方法 对1980年1月至2007年7月就诊于天津医科大学第二医院眼科的37例眼眶瘘管患者的病因、瘘管特点、影像学特征及治疗方法 逐一进行回顾性分析.结果 37例患者中因异物存留引发瘘管形成21例,包括植物性异物17例、医源性异物3例、花炮异物1例;眼眶囊肿9例,包括皮样囊肿5例、表皮样囊肿3例、额窦黏液囊肿l例;眼眶感染性炎症6例,包括骨髓炎5例、眼眶脓肿1例;嗜酸性肉芽肿1例.瘘管特点及影像学表现复杂,但具有一定规律性.治疗以手术清除病灶,切除瘘管为主,同时治疗毗邻结构病变,消灭复发根源.结论 眼眶瘘管形成的常见病因是异物存留、皮样囊肿和骨髓炎,其发病机制包括感染性炎症、先天性发育异常、手术及肿瘤.B型超声、CT、MRI检查对病灶的定位及定性诊断具有重要价值.正确判断病因,分析瘘管特点,是制定正确的治疗方案、根除瘘管的关键.     

转醛醇酶在不同发育阶段小鼠睾丸中的表达及其亚型的分离和鉴定

目的：研究转醛醇酶在不同发育阶段小鼠睾丸中的表达并分离鉴定其亚型，方法：应用SDS－聚丙烯酰胺胶电泳（SDS－PAGE）、蛋白免疫印迹（Western bloting)对转醛醇酶在不同发育阶段小鼠睾丸中的表达进行半定量分析，同时利用双向电泳免疫印变技术分离，鉴定转醛醇酶的亚型。结果：（1）转醛醇酶在生后1周小鼠睾丸中表达量平均比成年高47．8％（P＜0．005）。（2）双向电泳Wester blotting示小鼠睾丸转醛醇酶共有10余个亚型，生后1周小鼠睾丸中a8亚型表达量极低，而在成年小鼠睾丸中a8亚型高表达。结论：（1）生后1周小鼠生长发育快，合成代谢旺盛，转醛醇酶基因绝对表达量高。（2）a8亚型可能与生殖功能（精子发生、睾酮生成）密切相关。     

腭咽环扎术后阻塞性睡眠呼吸暂停低通气综合征上气道形态的研究

目的：探讨腭咽环扎术（VRLP）后阻塞性睡眠呼吸暂停低通气综合征（OSAHS）患者的上气道形态特征.方法：应用纤维鼻咽镜和头影测量技术检查了6例VRLP后并发OSAHS患者、6例VRLP后未发生OSAHS和6例未手术腭裂患者的上气道形态,测量了鼻咽腔直径、腭咽环扎处咽腔直径、口咽腔直径、舌根后咽腔直径、下咽腔直径、舌骨至下颌骨的距离等6个相关参数,并应用单因素方差分析进行组间比较.结果：未手术腭裂患者上气道形态光滑,VRLP后上气道在软腭后区和舌根后区出现2处狭窄,VRLP后OSAHS患者上气道鼻咽部明显狭窄变形,为腺样体肥大所致.结论：X线头影测量技术是一种简单实用的评估VRLP后OSAHS的方法,建议VRLP术前行此检查以排除腺样体严重增生患者.     

Osimertinib successfully combats EGFR-negative glioblastoma cells by inhibiting the MAPK pathway

Glioblastoma(GBM)patients have extremely poor prognoses,and currently no effective treatment available including surgery,radiation,and chemotherapy.MAPK-interacting kinases(MNK1/2)as the downstream of the MAPK-signaling pathway regulate protein synthesis in normal and tumor cells.Research has shown that targeting MNKs may be an effective strategy to treat GBM.In this study we investigated the antitumor activity of osimertinib,an FDA-approved epidermal growth factor receptor(EGFR)inhibitor,against patient-derived primary GBM cells.Using high-throughput screening approach,we screened the entire panel of FDA-approved drugs against primary cancer cells derived from glioblastoma patients,found that osimertinib(3μM)suppressed the proliferation of a subset(10/22)of EGFR-negative GBM cells(>50%growth inhibition).We detected the gene expression difference between osimertinib-sensitive and-resistant cells,found that osimertinib-sensitive GBM cells displayed activated MAPK-signaling pathway.We further showed that osimertinib potently inhibited the MNK kinase activities with IC50 values of 324 nM and 48.6 nM,respectively,against MNK1 and MNK2 kinases;osimertinib(0.3–3μM)dose-dependently suppressed the phosphorylation of eukaryotic translation initiation factor 4E(eIF4E).In GBM patient-derived xenografts mice,oral administration of osimertinib(40 mg·kg^?1·d^?1,for 18 days)significantly suppressed the tumor growth(TGI=74.5%)and inhibited eIF4E phosphorylation in tumor cells.Given the fact that osimertinib could cross the blood–brain barrier and its toxicity was well tolerated in patients,our results suggest that osimertinib could be a new and effective drug candidate for the EGFR-negative GBM patients.     

酶法和硫磷铁法测定黄疸血清胆固醇的比较

报告酶法和硫磷铁法测定黄疸血清中胆固醇的比较和偏差。结果显示两法测定清亮血清胆固醇无显著差异;测定黄疸血清则差异显著。此种偏差与血清黄疸指数正相关(r=0.624,P<0.01),与胆固醇浓度负相关(r=-0.605,P<0.01)。