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排序方式: 共有2697条查询结果,搜索用时 31 毫秒
991.
Viviana Ananian Pamela Tozzo Elena Ponzano Donato Nitti Daniele Rodriguez Luciana Caenazzo 《International journal of legal medicine》2011,125(3):327-332
In certain circumstances, tumour tissue specimens are the only DNA resource available for forensic DNA analysis. However,
cancer tissues can show microsatellite instability and loss of heterozygosity which, if concerning the short tandem repeats
(STRs) used in the forensic field, can cause misinterpretation of the results. Moreover, though formalin-fixed paraffin-embedded
tissues (FFPET) represent a large resource for these analyses, the quality of the DNA obtained from this kind of specimen
can be an important limit. In this study, we evaluated the use of tumoural tissue as biological material for the determination
of genetic profiles in the forensic field, highlighting which STR polymorphisms are more susceptible to tumour genetic alterations
and which of the analysed tumours show a higher genetic variability. The analyses were conducted on samples of the same tissues
conserved in different storage conditions, to compare genetic profiles obtained by frozen tissues and formalin-fixed paraffin-embedded
tissues. The importance of this study is due to the large number of specimens analysed (122), the large number of polymorphisms
analysed for each specimen (39), and the possibility to compare, many years after storage, the same tissue frozen and formalin-fixed
paraffin-embedded. In the comparison between the genetic profiles of frozen tumour tissues and FFPET, the same genetic alterations
have been reported in both kinds of specimens. However, FFPET showed new alterations. We conclude that the use of FFPET requires
greater attention than frozen tissues in the results interpretation and great care in both pre-extraction and extraction processes. 相似文献
992.
Campbell P Leopold JA Selwyn AP Sisto D 《Circulation. Heart failure》2011,4(3):369-73; discussion 374-7
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Gaggiano Carla Rigante Donato Sota Jurgen Grosso Salvatore Cantarini Luca 《Clinical rheumatology》2019,38(1):11-17
Clinical Rheumatology - Periodic fever, aphthous stomatitis, pharyngitis, and cervical adenitis (PFAPA) syndrome is the most frequent non-hereditary autoinflammatory disorder in childhood: Its... 相似文献
995.
da Silva LM Claro AP Donato TA Arana-Chavez VE Moraes JC Buzalaf MA Grandini CR 《Artificial organs》2011,35(5):516-521
The most commonly used titanium (Ti)-based alloy for biological applications is Ti-6Al-4V, but some studies associate the vanadium (V) with the cytotoxic effects and adverse reactions in tissues, while aluminum (Al) has been associated with neurological disorders. Ti-Nb alloys belong to a new class of Ti-based alloys with no presence of Al and V and with elasticity modulus values that are very attractive for use as a biomaterial. It is well known that the presence of interstitial elements (such as oxygen, for example) changes the mechanical properties of alloys significantly, particularly the elastic properties, the same way that heat treatments can change the microstructure of these alloys. This article presents the effect of heat treatment and oxygen doping in some mechanical properties and the biocompatibility of three alloys of the Ti-Nb system, characterized by density measurements, X-ray diffraction, optical microscopy, Vickers microhardness, in vitro cytotoxicity, and mechanical spectroscopy. 相似文献
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