室性早搏的快速检测方法

提出一种利用相关性和RR间期比相结合的快速室性早搏的检测算法.该算法具有一定的病人自适应性.通过MIT-BIH心律失常数据库的验证,该方法对Normal和PVC有很高的识别率.     

所有权与经营权分离是城市公立医院改革和发展的途径

传统卫生管理体制承袭了计划经济的所有特征,价格扭曲的宏观政策环境、资源的集中计划配置体制与公立医院在微观上的缺乏经营自主权三者并存.这种制度安排越来越不适应市场经济条件下卫生改革和发展的需要,而卫生经济体制改革始自微观经营机制,核心是两权分离和放权让利,这是自然的,同时也是正确的改革方向.     

Further evidence for a susceptibility locus for type 2 diabetes on chromosome 20q13.1-q13.2

We previously reported suggestive linkage between type 2 diabetes and markers in a region on chromosome 20q using data from a collection of 29 Caucasian families in which type 2 diabetes with middle-age-onset was segregated as an autosomal-dominant disorder. To map more precisely the susceptibility locus (or loci) within this broad region, we increased the family collection and genotyped all families for additional markers, both within the critical region and spaced over the rest of chromosome 20. Altogether 526 individuals (including 241 with diabetes) from the total collection of 43 families were included in the study. All individuals were genotyped for 23 highly polymorphic markers. Positive evidence for linkage was found for a 10-cM region on the long arm of chromosome 20q13.1-q13.2 between markers D20S119 and D20S428. The strongest evidence in two-point as well as multipoint linkage analysis (P = 1.8 x 10(-5)) occurred at the position corresponding to marker D20S196. The individuals with diabetes in the seven most strongly linked families had high serum insulin levels during fasting and 2-h post-glucose load periods. We did not find any evidence for linkage between type 2 diabetes and any other region on chromosome 20. In conclusion, our larger and more comprehensive study showed very strong evidence for a susceptibility gene for insulin-resistant type 2 diabetes located on the long arm of chromosome 20 around marker D20S196.     

大鼠晕船适应动物模型的建立

的　建立大鼠晕船适应动物模型。方法　将经过筛选的 4 0只SD雄性大鼠随机分为正常对照组、晕船组、晕船适应组及适应对照组 ,实验组动物进行模拟晕船刺激后分不同时间处死。主要以异食癖行为的发生与消失来判断大鼠晕船及适应的产生 ,同时结合大鼠晕船及适应发生前后行为的改变和血浆促肾上腺皮质激素 (ACTH)、β 内啡肽 (β EP)和肾上腺维生素C含量的变化来辅助证明大鼠晕船适应的产生。结果　在接受模拟晕船刺激的第 3天大鼠异食高岭土量 [(0 .8± 0 .0 2 )g]明显高于正常对照组 [(0 .0 3±0 .0 1)g],差异有显著性 (P <0 .0 1) ,从第 8天起大鼠异食高岭土量开始减少 ,到第 16天正常对照组和晕船适应组大鼠异食高岭土量的差异无显著性 (P >0 .0 5 )。晕船发生后 ,大鼠血浆ACTH[(16 4 7.83± 10 8.2 1)pg/ml]和β EP[(5 19.2 2± 6 3.35 )pg/ml]水平明显高于正常对照组 [分别为 (10 6 9.6 9± 15 8.96 )、(2 5 7.91±4 3.12 )pg/ml],晕船适应组、适应对照组及正常对照组大鼠血浆ACTH和β EP含量的差异无显著性 (P >0 .0 5 )。晕船组大鼠肾上腺维生素C含量 [(2 .89± 0 .30 ) μg/mgpro]明显低于正常对照组 [(3.38±0 .31) μg/mgpro],正常对照组、晕船适应组和适应对照组间大鼠肾上腺维生素C含量的差异     

孤立型脉络膜血管瘤的超声诊断

目的 探讨孤立型脉络膜血管瘤的超声诊断价值。方法 使用标准化A超联合B超诊断6例脉络膜血管瘤。结果 脉络膜血管瘤B超显示实性占位病变，回声与正常脉络膜组织回声一致；A超表现为病变内高反射波。结论 超声检查是诊断脉络膜血管瘤有价值的辅助手段。     

白内障手术前后结膜囊细菌学分析

目的：探讨白内障术前、术后结膜囊细菌学分布状态，为临床用药提供依据。方法：将无睑缘炎、结膜囊外观清洁、泪道冲洗通畅的白内障住院患者94例分别于入院未用抗生素眼液点眼前、手术消毒前及术后第1天拆开眼垫换药前进行结膜囊细菌培养。结果：未用抗生素眼液点眼前的结膜囊(正常结膜囊)细菌培养阳性率为56．4％，经0．3％氟哌酸眼液点眼后结膜囊细菌培养阳性率为0，但术后第1天拆开眼垫后结膜囊的细菌培养阳性率为5．3％。结论：正常结膜囊可以有各种致病菌或非致病菌存在，即使术前结膜囊细菌培养为无菌生长，但术后第1天仍然可能有结膜囊细菌存在，提示白内障术前及术后均应加强抗生素眼液点眼，以减少眼内炎的发生。     

青春双歧杆菌和嗜酸乳酸杆菌对急性期和恢复期结肠炎小鼠Th1/Th2亚群的影响

Objective To observe and analyze the effect of B. Adolescentis and L. Acidophilus on the proportion of Th cell Th1/Th2 in peripheral blood of UC-mice in acute stage and recovery stage. Method 40 BABL/c mice were induced by 3% DSS water for 7 clays free drinking and then with distilled water for 10 days. They were randomly allocated in 4 groups: NS group, SASP group, BF0624 group and LT0637 group, also the fifth group-10 normal animals. The blood of mice were collected by removing their eyes at day 8 and day 18, and then the mononuclear cells were iso]atecl. The proportion of Th1/Th2 was analyze through flow eytometry,hy labeling the specific antibody ot Th cellular membrane with the CD4 antibody, and the cytoplastie antigen of Thl or Th2 with IIA antibody or IF'N-γ antibody. Result The proportion of Th1/Th2 in normal mouse was 0. 84 -0. 94,and it raised up in DSS-mice at both acute stage and recovery stage. It decreased unequally after 7 or 17 days'B, adoleseentis, L. Acidophilus and SASP treatment, but that of all three groups were lower than NS group (2.21±0. 83). Even the proportion got close to the normal animals after 17 days'L. Acidophilus -treated. Conclusion The proportion of Thl/Th2 increased at the acute stage and recovery stage of DSS-mice. Both B. Adoleseentis and L. Acidophilus had more effective than SASP on decreasing the proportion of Th1/Th2 at two stages,ospecially L. Acidophilus.     

肝硬化患者血清胆碱酯酶与透明质酸和Child-Pugh分级的关系

目的 探讨肝硬化患者血清胆碱酯酶(CHE)活性与透明质酸(HA)和Child-Pugh分级的相关性。方法 采用速率法和放射免疫法(RIA)测定了81例肝硬化患者、对照组30例血清CHE活性和肝纤维化标志物HA水平。结果 肝硬化Child-A、Child-B、Child-C级患者，CHE活性依次降低，肝纤维化标志物HA水平依次升高，且相差显著。CHE活性与HA水平呈显著负相关。CHE活性降低和HA水平升高与Child-Pu小分级具有一致性。结论 血清CHE活性水平可反映肝硬化的严重程度，血清CHE活性是评估肝硬化患者肝组织纤维化敏感而准确的指标，对评估手术治疗风险、病情变化及预后有一定的临床参考价值。     

皮脂腺细胞体外培养及生长调节的研究进展

皮脂腺是皮肤重要的附属器。皮脂腺体外培养模型的建立为研究相关因子如细胞因子、生长激素及药物等对皮脂腺形态功能、生长发育及病理改变的作用提供了良好的平台。本文对皮脂腺细胞体外培养的实验方法、体外生长特性及影响皮脂腺细胞增殖分化的相关因素作简要综述。     

经皮胆红素测量仪在早期正常新生儿黄疸监测中的应用

目的 :探讨经皮胆红素测量仪对早期新生儿黄疸动态观测及不同部位在动态观测中的实用价值。方法 :本院产科 2 0 0 2年 2月～ 2 0 0 3年 4月出生 ,连续监测 5 d～ 7d的正常足月新生儿 174例。采用国产 JD- 1型经皮黄疸仪 ,生后第 1天开始每日晨间沐浴前进行测量 ,每次测量 4个部位 :前额眉间部 ,胸骨中部 ,小腿外侧中部 ,足心前 2 / 3部。结果 :测量值最高读数为 2 2 ,最低读数为 5 ,以额部读数 15为界定值判断黄疸发生率为 86 %。额部、胸部、小腿每日读数平均值从出生第 1天起逐渐上升 ,第 5天达高峰 ,而后缓慢下降。各部位读数第 90百分位数峰值依次为 19.75、18.97、16 .35、11.93。结论 :经皮胆红素测量仪对早期新生儿黄疸动态观测有良好的临床应用价值。额部黄疸出现最早 ,较适合于研究新生儿黄疸发生率 ;胸部曲线与额部平行 ,易于操作 ,重复性好 ,可作为测量的主要部位 ;小腿外侧中部可观测黄疸程度 ,了解新生儿高胆红素血症的发生情况