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751.
752.
Enhanced expression of interleukin-1alpha and tumor necrosis factor receptor-associated protein 1 in ileal tissues of cattle infected with Mycobacterium avium subsp. paratuberculosis 下载免费PDF全文
Infection with Mycobacterium avium subsp. paratuberculosis is associated with high levels of morbidity, decreased production, and early culling in dairy cattle. Clinical symptoms of Johne's disease include persistent diarrhea, inappetence, and resultant weight loss due to chronic inflammation of the small intestine. Although the presence or absence of intestinal lesions cannot be used as a definitive indicator of M. avium subsp. paratuberculosis infection, most infected cattle exhibit significant changes to intestinal mucosa, with the focus of pathology surrounding the ileal cecal junction. Typical pathology of M. avium subsp. paratuberculosis infection includes inflammation, thickening of the lumenal wall, and hyperplasia in draining lymph nodes. To further understand the pathology of Johne's disease, we compared the gene expression profiles of ileal tissues from Johne's disease-positive (n = 6), and Johne's disease-negative (n = 5) Holstein cattle. Gene expression profiles were compared with a bovine total leukocyte (BOTL-3) cDNA microarray. Genes that were expressed at significantly higher levels (>1.5-fold; P < 0.05) in tissues from Johne's disease-infected animals relative to noninfected animals included those encoding tumor necrosis factor receptor-associated protein 1 (TRAF1), interleukin-1alpha (IL-1alpha), MCP-2, N-cadherin, and beta1 integrin (CD29). Dramatic upregulation of IL-1alpha (21.5-fold) and TRAF1 (27.5-fold) gene expression in tissues of Johne's disease-positive cows relative to tissues from control cows was confirmed by quantitative real-time PCR. Western blot analysis confirmed that IL-1alpha and TRAF1 mRNA levels resulted in increased protein expression in tissues of Johne's disease-positive cattle relative to tissues from control cattle. High levels of IL-1alpha can produce symptoms similar to those found in clinical Johne's disease. Taken together, the data presented in this report suggest that many outward symptoms of Johne's disease may be due to IL-1alpha toxicity. In addition, enhanced levels of TRAF1 could result in cells within the lesions of Johne's disease-positive cattle that are highly resistant to TNF-alpha-induced signaling. 相似文献
753.
BACKGROUND: Asthma clinics have become widespread in general practice with
nurses now playing an important role in asthma review. However, little is
known about training of nurses carrying out reviews and how this affects
the nurse role in patient management. OBJECTIVES: We aimed to discover the
level of asthma training of practice nurses carrying out review of adult
asthma patients in one Health Authority and to see if this has any effect
on their perception of their role. METHOD: All 187 practice nurses in
Grampian were sent a postal questionnaire investigating how asthma review
is organized in general practice, their role in review and the asthma
training they had received. Personal interviews were carried out with 17
nurses, exploring in more depth the topics covered in the questionnaire.
RESULTS: A total of 167 nurses from 92% of the practices in Grampian
responded, of whom 61% carried out asthma reviews. Among nurses carrying
out reviews 71% did so on their own. 49% of nurses had or were training for
advanced asthma qualification. Nurses without an asthma qualification were
significantly more likely to feel that their training was not sufficient
for their asthma related tasks (54% versus 11%, P = 0.0002). Nurses without
advanced asthma qualifications were less likely to provide or review a
self-management plan (29% versus 49%, P = 0.01), to review patient PEF
recording (38% versus 65%, P < 0.01), to discuss patient worries (75%
versus 94%, P < 0.05) or to make the initial diagnosis of asthma (24%
versus 76%, P < 0.005). Nurses were unlikely to view their role as fully
responsible unless they had an asthma qualification (13% versus 49%, P <
0.001). CONCLUSION: Nurses without advanced asthma qualifications do not
feel fully confident in responsibility for patient management. Nurses
without training are more likely to only carry out routine monitoring at
reviews while nurses with asthma training are more likely to actively
develop patient self- management skills. This suggests that nurses should
be supported to obtain asthma qualifications if they are to give the best
possible care to asthma patients.
相似文献
754.
Influence of the amount of platelet-bound IgG on platelet survival and site of sequestration in autoimmune thrombocytopenia 总被引:1,自引:0,他引:1
Seventeen patients with idiopathic autoimmune thrombocytopenic purpura were studied in order to establish whether the amount of platelet-bound antibody influenced the rate and site at which these platelets were destroyed. Platelet-bound antibody was measured by a quantitative antiglobulin consumption technique, and platelet survival and sites of sequestration were determined by the use of 51Cr-labeled platelets and surface radioactivity measurements. A correlation significant at the 5% level was observed between the amount of platelet-bound antibody and the rate of platelet destruction. A highly significant correlation (p less than 0.001) existed between antiplatelet antibody and spleen: liver surface radioactivity ratios, demonstrating that greater amounts of antibody bound to platelets result increasingly in their destruction within the liver. 相似文献
755.
The developmental age of an embryo in the first trimester ofpregnancy is generally determined by ultrasound scanning and/orby calculation from menstrual age. In the original studies,validation of the estimate of gestational age by ultrasoundwas not possible as the exact date of conception was unknown.Variation in growth rates of identically aged fetuses has previouslybeen reported after assisted conception and with the use ofultrasound scanning. As these pregnancies were ongoing the accuracyof the scanning results could not be determined. Comparisonof scanning and direct measurements after termination of pregnancyand menstrual age were carried out to determine the accuracyin fetal dating. The results suggest that the use of ultrasoundscanning to determine gestational age is of less use than previouslythought, and that the use of menstrual age is severely limited. 相似文献
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759.
Clinical safety of gadopentetate dimeglumine 总被引:3,自引:1,他引:2
760.