消抗汤治疗免疫性反复自然流产抗心磷脂抗体阳性46例

目的:观察消抗汤治疗免疫性反复自然流产抗心磷脂抗体阳性的临床疗效。方法:将65例免疫性反复自然流产抗心磷脂抗体阳性的患者,根据随机法按2:1分为试验组(46例,采用消抗汤加减治疗)和对照组(19例,采用阿斯匹林治疗),治疗8周后观察2组患者症状调查问卷评分和抗心磷脂抗体检验变化情况。结果:试验组46例,抗体转阴29例,有效率63.04%;对照组19例,抗体转阴6例,有效率31.58%;试验组治疗后的症状调查问卷平均积分与治疗前相比有显著下降,对照组治疗后的症状调查问卷平均积分与治疗前相比无显著差异。试验组总疗效明显优于对照组(P<0.05)。结论:通过临床试验观察表明,中药消抗汤对免疫性反复自然流产患者抗心磷脂抗体的转阴以及症状的改善具有显著的疗效,明显优于阿斯匹林的治疗效果。     

HPLC指纹图谱结合化学模式识别评价舒筋活血制剂的整体质量

目的建立舒筋活血制剂(舒筋活血胶囊、舒筋活血片)HPLC指纹图谱的方法,为其整体质量评价提供参考。方法色谱柱为Agilent Poroshell 120 SB-C18(150 mm×4.6 mm,2.7μm),以乙腈-0.2%甲酸水溶液为流动相,进行梯度洗脱;体积流量0.5mL/min,检测波长277nm,柱温30℃。采用"中药色谱指纹图谱相似度评价系统"(2012版)考察23批舒筋活血制剂的相似度,结合主成分分析(principal component analysis,PCA)、聚类分析(cluster analysis,CA)以及正交偏最小二乘判别分析(orthogonal partial least squares discriminant analysis,OPLS-DA)对舒筋活血制剂进行整体质量评价。结果舒筋活血制剂的HPLC指纹图谱中18批样品(S1～S17和S23)的相似度0.900,通过PCA、CA和OPLS-DA,23批样品聚成3类,S1～S16来自同一厂家为一类;S23和S17为一类;S18～S22为一类,并从10个共有峰中确定差异较大的4个共有峰,分别为1、3(5-羟甲基糠醛)、6(苯乙酸)和10(4-甲氧基水杨醛)号峰。结论所建立的指纹图谱结合化学模式识别方法稳定、简单,可用于评价舒筋活血制剂质量。     

单侧隐睾大鼠对侧睾丸组织中SCF/c-kit基因表达变化及意义

Objective To investigate the pathologic changes and expressions of SCF and c-kit in the contralateral testes in rat model of unilateral cryptorchidism. Methods Thirty male SD rats were maintained under controlled temperature and constant photoperiodic conditions with access to food and water. The rats were randomly assaigned to the control group and the experimental unilateral cryptorchidism group. The left testis of the rats in the unilateral cryptorchidism group was placed into the abdominal cavity. The control group rats were subjected to sham surgery. Three months later, the rats were sacrificed and their right testes were harvested. The pathological changes were observed under microscope. The mRNA and protein expression of SCF and c-kit were also investigated using quantitative Real-time RT-PCR, Western blotting and immunohistochemical staining. Apoptotic germ cells were detected by TUNEL staining. Results All rats survived to the endpoints. The right testes of rats of the unilateral cryptorichid group were smaller than those of the control group. Normal testes of the control group rats manifested active spermatogenesis and orderly arrangement of germ cells.However, disordered and sloughed germinal cells with less distinct seminiferous tubule borders were observed in the contralateral testes of rats with experimental unilateral cryptorichid under the microscope. Cmpared with the control testes, the mRNA and protein expression of SCF and c-kit of the contralateral testes of rats with experimental unilateral cryptorichid was decreased (P＜0.05). The apoptotic germ cells were increased (19.7±3.83 vs 5. 4 ± 1.02, P＜0. 05). The SCF/c-kit expression was positively correlated with the germ cell apoptosis (P＜0. 01 ). Conclusions The decreased expression of SCF and c-kit and increased germ cell apoptosis are found in the contralateral testes, which may contribute to thc infertility in the rat model of experimental unilateral cryptorchidism.     

子宫内膜异位症与抗碳酸酐酶抗体的关系

目的 :探讨抗碳酸酐酶Ⅰ、Ⅱ抗体与子宫内膜异位症的关系。方法 :应用酶联免疫吸附 (ELISA)法分别检测内异症 32例、其他妇科疾病 (妇科组 ) 2 0例及正常妇女 12例血清抗碳酸酐酶抗体水平。结果 :(1)内异症患者血清抗碳酸酐酶Ⅰ、Ⅱ抗体阳性率(87.5 %、5 3.1% )高于妇科组 (2 5 %、15 % ) ,两组差异有显著性 (P <0 .0 1) ;(2 )内异症患者血清抗碳酸酐酶Ⅰ、Ⅱ抗体阳性率明显高于正常妇女组 (0 %、0 % ) ,两组差异有显著性 (P<0 .0 1) ;(3)妇科组及正常妇女组血清抗碳酸酐酶Ⅰ、Ⅱ抗体阳性率较低 ,两组差异无显著性 (P >0 .0 5 )。结论 :检测抗碳酸酐酶抗体对诊断子宫内膜异位症及探讨其发病机制有重要价值     

海洛因对大鼠谷氨酸脱氢酶基因表达的影响

目的:研究海洛因对大鼠谷氨酸脱氢酶(GDH)基因表达的影响。方法:40只成年♂Wistar大鼠随机分为4组:对照组腹腔注射(ip)生理盐水9d;给药3d组ip海洛因3d;给药9d组ip海洛因9d;停药组ip海洛因9d后停药8d。采用生化自动分析系统检测血浆及组织中GDH活性,逆转录聚合酶链反应法(RT-PCR)检测组织中GDHmRNA的含量。结果:血浆中GDH活性在海洛因给药期间逐渐增高,停药8d后仍无明显降低;大脑组织GDH活性在海洛因给药3d、海洛因给药9d及停药8d后均显著低于对照组(P<0·01);肝脏GDH活性在海洛因给药过程中降低(P<0·01),停药8d后恢复到对照组水平;小肠GDH活性在海洛因给药9d后显著高于对照组(P<0·05),停药8d后小肠GDH活性继续升高并明显高于对照组(P<0·01)和海洛因给药9d组(P<0·05)。大脑、肝脏和小肠GDHmRNA含量分别与其GDH活性变化趋势一致。结论:海洛因对大鼠不同组织GDH基因表达影响不同。     

肾衰养真胶囊对慢性肾衰竭营养不良大鼠下丘脑神经肽Y mRNA及原阿片黑皮素mRNA表达的影响

目的 观察肾衰养真胶囊对慢性肾衰竭(CRF)营养不良大鼠下丘脑神经肽Y(NPY)mRNA及原阿片黑皮素(POMC)mRNA的表达及其营养状态的影响.方法 采用含0.5%腺嘌呤及4%酪蛋白饲料联合喂养的方法制作CRF营养不良大鼠模型,观察其营养不良发生时间,符合CRF营养不良模型的随机分为模型组、肾衰养真胶囊组和开同组.治疗4周后,检测下丘脑NPY mRNA和POMC mRNA表达水平.结果 肾衰养真胶囊组大鼠营养状况明显改善,下丘脑的NPY mRNA表达上调及POMC mRNA表达下调.结论 肾衰养真胶囊可改善CRF营养不良大鼠的营养状况,其机制可能是通过上调下丘脑NPY mRNA表达,下调下丘脑POMC mRNA表达,从而促进大鼠摄食.     

Silencing of Nesprin‐2 inhibits the differentiation of myofibroblasts from fibroblasts induced by mechanical stretch

Mechanical force plays a pivotal role in the pathogenesis of hypertrophic scar (HTS). Dermal fibroblasts and myofibroblasts are the key cells involved in HTS. Myofibroblasts in HTS possess different biochemical and biophysical characteristics by which myofibroblasts are often distinguished from fibroblasts. The role of mechanotransducers outside the nucleus in the pathogenesis of HTS has been reported in many studies. However, the role of Nesprin‐2 in HTS is not clear. Hence, we aim to construct a cell model of HTS and explore the role of Nesprin‐2 in this process. Myofibroblasts and fibroblasts were isolated from HTS and healthy skin tissues of the same patient. Fibroblasts were exposed to cyclic stretch with 10% magnitude and a frequency of 0.1 Hz for 3 days, 5 days, and 7 days, respectively. After the cell model was confirmed, fibroblasts transfected with siRNA targeting human Nesprin‐2 were exposed to cyclic stretch. The mechanical behaviour and biochemical reaction of the dermal fibroblasts were analysed. The stretched fibroblasts at day 5 showed the same mechanotransductive and biochemical features as unstretched myofibroblasts. Mechanical strain could induce the myofibroblasts differentiation and a cell model of HTS was established successfully at day 5. The expressions of lamin A/C, alpha‐smooth muscle actin, transforming growth factor beta 1, and collagen type I in fibroblasts were reduced by the silencing of Nesprin‐2. Mechanical strain could induce the myofibroblasts differentiation and silencing of Nesprin‐2 could block the mechanical stimulation of terminal myofibroblasts differentiation. Nesprin‐2 might be a potential target to treat the HTS.     

龙江韩氏妇科治疗早发性卵巢功能不全经验

龙江韩氏妇科认为,肾虚是早发性卵巢功能不全发病之根本,肝郁气滞、瘀血内结为发病之关键.治疗本病以补肾疏肝、活血调经为大法,以百灵育阴汤为主,配合雌激素、孕激素、中成药及膏方分阶段治疗,并在药物治疗的同时配合药膳疗法及心理疗法.     

短暂性脑缺血发作中医证候量化诊断标准研究

目的：建立短暂性脑缺血发作中医证候量化诊断标准。方法：根据文献研究、专家咨询、临床流行病学调查获得短暂性脑缺血发作四诊信息。将493例临床患者随机分为运算组（370例）和考核组（123例）,以基于熵的复杂系统分划方法提取运算组患者症状信息,分析证候之间组合规律,确立症状对证候贡献度,以诊断性试验ROC曲线分析方法建立诊断阈值,并依据考核组数据对其进行前瞻性检验。结果：短暂性脑缺血发作存在以络气郁滞、络气虚滞、痰湿、血瘀、阴虚、火热等基本证候,分别确立了不同症状对证候诊断的贡献度,建立了诊断阈值,前瞻性检验显示其具有良好的灵敏度、特异度及符合率。结论：基于熵的复杂系统分划方法可用于中医证候的量化研究,建立的短暂性脑缺血发作量化诊断标准具有良好的诊断效能,为中医证候规范化及进一步开展络病与血管病变相关性研究奠定了基础。     

应激性认知障碍小鼠脑内同型半胱氨酸的代谢变化研究

目的 通过检测应激小鼠海马和前额叶皮质同型半胱氨酸(Hcy)及其代谢酶水平,了解应激对脑内Hcy代谢的影响,为应激性认知障碍的Hcy损伤机制补充新的证据.方法 24只C57小鼠随机分为对照组和3周慢性束缚应激组,采用高压液相色谱分别检测血浆以及海马和前额叶皮质组织匀浆液中Hcy水平,采用皮质酮酶联免疫吸附(ELISA)...