Treatment of 30 cases of vitiligo by cupping method plus external application of Chinese herbs

在皮损区拔罐,然后外涂中药治疗白癜风患者30例,对照组予西药治疗,3个疗程后,治疗组总有效率96.7%,对照组总有效率76.7%.治疗组疗效好于对照组(P＜0.05).     

再谈肺癌中血管生成与其预后的关系

目的:研究肺癌中血管内皮生长因子(vascularendothelialgrowthfactorVEGF)、激酶插入结构域受体(ki鄄naseinsertdomainingreceptorKDR)表达水平和微血管密度(Micro-vasculardensityMVD)及其与肺癌预后的关系。方法:采用免疫组化方法(LSAB法),检测114例肺癌组织中的VEGF、KDR表达水平及MVD。结果:肺癌组织中VEGF、KDR高表达组患者5年生存率分别为18.18%和21.19%,显著低于低表达组61.19%和52.74%(P<0.01);MVD高密度组患者5年生存率为17.27%,显著低于低密度组52.74%(P<0.01)。VEGF、KDR表达水平及MVD与生存期均呈显著负相关(P<0.01),其相关强度依MVD、VEGF、KDR顺序逐渐降低。多因素分析表明,pTNM分期仍是预测肺癌预后最有价值的指标,但VEGF、KDR表达水平,MVD也是判断肺癌预后较有价值的指标。VEGF、KDR表达水平越高,MVD越大,其无瘤生存期和5年生存率越短。结论:肺癌中VEGF、KDR基因的表达异常及MVD的增大在肺癌的发生、发展、转移和预后中可能起重要作用,检测肺癌中VEGF、KDR基因表达水平及MVD有助于预测肺癌预后。     

Non-invasive detection of fecal protein kinase C betaII and zeta messenger RNA: putative biomarkers for colon cancer

We have developed a non-invasive method utilizing feces, containing sloughed colonocytes, as a sensitive technique for detecting diagnostic colonic biomarkers. In this study, we used the rat colon carcinogenesis model to determine if changes in fecal protein kinase C (PKC) expression have predictive value in monitoring the neoplastic process. Weanling rats were injected with saline or azoxymethane (AOM) and 36 weeks later fecal samples and mucosa were collected, poly A+ RNA isolated, and quantitative RT-PCR performed using primers to PKC betaII and zeta. Fecal PKC betaII and zeta mRNA levels were altered by the presence of a tumor, with tumor-bearing animals having a 3-fold higher (P < 0.05) PKC betaII expression as compared with animals without tumors. In addition, AOM-injection increased mucosal PKC betaII mRNA expression compared with saline controls. No effect of tumor incidence on mucosal PKC betaII expression was observed. In contrast, fecal PKC zeta expression was 2.5-fold lower (P < 0.05) in animals injected with azoxymethane versus saline. Since tumor incidence exerts a reciprocal effect on fecal PKC betaII and zeta mRNA expression, data were also expressed as the ratio between PKC betaII and zeta. The isozyme ratio was strongly related to tumor incidence, i.e. ratio for animals with tumors was 2.18 +/- 1.25, animals without tumors was 0.50 +/- 0.16, P = 0.025. We demonstrate that the expression of fecal PKC betaII and zeta may serve as a noninvasive marker for development of colon tumors. A sensitive technique for the detection of colon cancer is of importance since early diagnosis can substantially reduce mortality.      

Role of cyclin E and p53 expression in progression of early gastric cancer

Background. To elucidate the role that cyclin E overexpression plays in the progression of early gastric cancer, we examined the expression of cyclin E and p53, as abnormal p53 expression is linked with cyclin E overexpression in exerting adverse affects on the cell cycle. Methods. Specimens from 108 early gastric cancers were stained by an immunohistochemical method, using anti-cyclin E and anti-p53 antibodies. Results. The positivity rate of cyclin E expression in early gastric cancer was 33% (36/108). Cyclin E-positive tumors invaded more deeply (P < 0.05), infiltrated lymphatic vessels more frequently (P < 0.01), showed a higher incidence of differentiated cancer (P < 0.01), and more often expressed p53 (P < 0.01) than cyclin E-negative tumors. Differentiated cancers showing coexpression of cyclin E and p53 were more likely to metastasize to the lymph nodes. Conclusions. Overexpression of cyclin E may promote the progression of early gastric cancer. Received for publication on Apr. 27, 1998; accepted on Nov. 17, 1998     

早期隔上霍奇金淋巴瘤斗篷野与累及野放疗联合化疗比较

目的:比较早期隔上霍奇金淋巴瘤(HD)斗篷野与累及野放疗联合化疗的疗效.方法:对35例早期HD患者,先予化疗4个疗程,然后随机分为常规斗篷野放疗(20例)或累及野放疗(15例),剂量为大野30Gy,局部肿块区加至40Gy,放疗后再化疗2个疗程.结果:中位随访58个月.3年总生存率为94.3%.无复发生存率为80.0%.两组3年总生存率及无复发生存率无明显差异.结论:早期隔上HD在化疗基础上,累及野与斗篷野放疗疗效相似,并发症较低.     

肿瘤转移相关基因与周围型肺癌CT征象的关系研究

摘要目的：探讨VEGF和nm23-H1基因表达水平与周围型肺癌的病理生理学特征和CT征象之间的关系。方法：回顾性分析确诊的47例周围型肺癌患者CT表现．同时采用免疫组化方法(LSAB法)检测47例肺癌组织中的VEGF和nm23-H1表达水平。结果：1)VEGF、nm23-H1表达水平与原发肿瘤大小、淋巴结转移状态，pTNM分期均有密切关系(P＜0．05)。2)VEGF与nm23-H1表达水平呈负相关(P＜0.05)。3)VEGF基因的高表达和nm23-H1基因的低表达均与分叶征、纵隔淋巴结转移有关(P＜0．05)，且VEGF的高表达也与空洞征有关：二者表达水平与毛刺征、空泡征、胸膜凹陷征和支气管征均无关(P＞0．05)。4)深分叶征、空泡征和支气管征与肿瘤大小有关(P＜0．05)，而与毛刺征、胸膜凹陷征和纵隔淋巴结转移均无关(P＞0.05)。结论：VEGF过度表达和nm23-H1低表达均与周围型肺癌的分叶征、空洞坏死征和纵隔淋巴结转移有密切关系。     

人白细胞介素1β(IL-1β)在诱导人黑色素瘤A375-S2细胞凋亡过程中的细胞周期阻滞作用和对线粒体Bcl-2家族蛋白的调节

目的研究人白细胞介素1β(interleukin-1β,IL-1β)体外诱导人黑色素瘤A375-S2细胞凋亡的机制。方法通过Hoechst33258荧光染色,观察A375-S2细胞在IL-1β作用下的形态学变化。使用琼脂糖凝胶电泳法检测IL-1β对细胞DNA降解的影响。应用流式细胞分析技术研究IL-1β对A375-S2细胞周期的影响。利用Westernβlot方法检测IL-1β对细胞线粒体内Bcl-2家族蛋白表达的调节作用。结果IL-1β(1nM)能够诱导A375-S2细胞凋亡,72h时细胞体变小,细胞核呈现固缩、断裂,并出现因凋亡引起的DNA梯状条带。IL-1β可将细胞周期阻滞在G0/G1期,并具有时间依赖性。IL-1β诱导细胞凋亡过程中,抗凋亡蛋白Bcl-2和Bcl-xL的表达减少,促凋亡蛋白Bax的表达增加。结论IL-1β通过将细胞周期阻滞在G0/G1期,上调线粒体内Bax/Bcl-2和Bax/Bcl-xL的表达比例诱导A375-S2细胞凋亡。     

芙蓉Cu200C宫内节育器系统评估

目的：系统评估芙蓉（FR）Cu200C IUD的安全性、有效性和可接受性。方法：通过电子和手工检索查阅1988年4月～2006年5月国内外发表的相关文献，按照循证医学的原则进行筛选和评估。结果：共检索到22篇相关文献，按标准纳入5篇。纳入文献的结果显示。置器后1年，FRCu200C IUD的累积妊娠率略高于母体乐（ML）Cu375 IUD，因症取出率也趋向高于MLCu375 IUD；FRCu200C IUD的经量增多发生率低于MLCu375 IUD；置器后1、2、5年FRCu200C IUD的累积续用率均趋向低于MLCu375 IUD。结论：FRCu200C IUD的临床效果，尤其是有效性，接近或略差于MLCu375 IUD。建议在推广使用FRCu200C IUD前，对其开展多中心随机对照临床研究，进一步证实其安全性、有效性和可接受性；建议由非FRCu200C IUD研制单位主持临床试验，观察年限应≥5年。     

止咳清肺口服液对25例慢性阻塞性肺疾病患者痰液炎症细胞白细胞介素8、肿瘤坏死因子α的影响

目的:观察中药止咳清肺口服液治疗慢性阻塞性肺疾病(COPD)气道炎症的效果.方法:50例稳定期COPD患者随机分成治疗组25例,对照组25例.两组氧疗、强心、利尿、止咳平喘等常规治疗相同.治疗组加止咳清肺口服液20ml,每日3次,连服6周.治疗前后记录临床症状计分,检测肺功能、痰液炎症细胞计数、分类和白细胞介素8(IL-8)、肿瘤坏死因子α(TNF-α)的变化.结果:治疗组临床症状计分、1秒钟用力呼吸容积(FEV1)和FEV1/用力肺活量(FVC)均有明显改善(P＜0.05或P＜0.01),而对照组治疗前后变化不明显.痰液中的细胞总数、中性粒细胞和IL-8、TNF-α水平比治疗前均有显著下降(P＜0.05或P＜0.01),而对照组下降不明显.结论:止咳清肺口服液治疗COPD气道炎症疗效明显.     

田维柱教授关于透穴应用的见解

田维柱教授从事针灸的医疗、教学和科研工作30余载,经验颇丰,临证治疗尤以透穴针法应用得心应手.针对目前针灸发展的现状,田师结合自己的教学经验及治疗心得,提出了有关腧穴的应用操作应与透穴相结合并形成规范的设想.