T细胞及相关免疫分子在淋巴细胞趋化因子增强肿瘤自杀基因疗法中的作用

本室以往的研究表明,用腺病毒作为载体将大肠杆菌胞嘧啶脱氨酶(CD)基因与小鼠淋巴细胞趋化因子(Itn)基因联合体内转染,具有显著的抗肿瘤效应.本文对其免疫机理进行了进一步研究,发现CT26结肠腺癌细胞体外经过CD/Ltn基因转染并给予前体药物5-FC后,CT26结肠腺癌细胞表达CD80和CD54分子明显增加,提示经CD自杀基因和Ltn基因联转移后,肿瘤细胞免疫原性增加.荷瘤小鼠体内经联合治疗后,小鼠脾细胞分泌IL-2和IFN-γ明显增加.体内用抗CD4、CD8抗体阻断实验研究结果的表明,联合应用自杀基因和Ltn基因治疗主要通过诱导CD8~ T细胞发挥抗肿瘤作用.     

Effects of Electroacupuncture on the Number of NK-1R／IL-1β Double Labeled Immunofluorescent Positive Cells in th_e Epidermal and Subcutaneous Tissue Around the Ankle Joint of Adjuvant Monoarthritis in Rats

Adjuvant monoarthritis was induced by the injection of 50μl Complete Freund‘s Adjuvant（CFA) into ankle joint of adult SD rats. Electroacupuncture (EA)was performed on Huantiao (GB30)and Yanglingquan (GB 34) (0.5-1.5 v, 4-16 Hz, 30 min) after the injection.     

Postnatal development of NR1, NR2A and NR2B immunoreactivity in the visual cortex of the rat

N-Methyl-D-aspartate receptors (NMDARs) are critically involved in some types of synaptic plasticity. The NMDAR subunits NR1, NR2A and NR2B are developmentally regulated, and it has been proposed that developmental changes in their expression may underlie developmental changes in cortical plasticity. Age-dependent change in cortical plasticity is most commonly measured by the monocular deprivation effect, which occurs during a critical period between P22 and P50 in the rat. Although the development of NMDAR subunits has been studied from birth through the fourth postnatal week, there is only meager information from older ages when visual plasticity ends. We hypothesized that there will be significant age-dependent change in expression of NR1, NR2A or NR2B between P22, when the cortex is plastic, and P90, when it is not. We applied specific antibodies recognizing NR1, NR2A and NR2B to the primary visual cortex at P14, P22, P30, P45 and P90. We found age-dependent changes in NR1-IR that were negatively correlated with changes in NR2A-IR; these subunits are not regulated in unison. In contrast, NR2A-IR and NR2B-IR were positively correlated. NR2A-IR and NR2B-IR both passed through a developmental minimum around P45, then recovered to approximately their P22 level. NR1-IR passed through a maximum at P45. There were no significant differences between P22 and P90. These results do not support the simple hypothesis that the loss of plasticity corresponds to a simple transition from juvenile levels of NMDAR subunit proteins to new adult levels. On the other hand, the results do confirm the hypothesis that there are significant changes in processing of NMDAR proteins during the time that plasticity is lost. How these changes of IR relate to synaptic transmission and plasticity needs to be clarified.     

Characterization of an immortalized human vaginal epithelial cell line

PURPOSE: Adherence of type 1 piliated Escherichia coli to vaginal mucosa plays a major role in the pathogenesis of ascending urinary tract infections (UTIs) in women. Progress in understanding the mechanism of adherence to the vaginal surface could be enhanced by the utilization of well-characterized vaginal epithelial cells. The objective of this study was to immortalize vaginal epithelial cells and study their bacterial adherence properties. MATERIALS AND METHODS: Primary vaginal cells were obtained from a normal post-menopausal woman, immortalized by infection with E6/E7 genes from human papillomavirus 16 (HPV 16) and cultured in serum free keratinocyte growth factor medium. RESULTS: Positive immunostaining with a pool of antibodies to cytokeratins 1, 5, 10 and 14 (K1, K5, K10 and K14) and to K13 confirmed the epithelial origin of these cells. The immortalized cells showed binding of type 1 piliated E. coli in a pili specific and mannose sensitive manner. CONCLUSION: This model system should facilitate studies on the interaction of pathogens with vaginal mucosal cells, an essential step in the progression of ascending UTIs in women.     

Distinctive gene expression profiles by cDNA microarrays in endometrioid and serous carcinomas of the endometrium.

Endometrial carcinomas are classified by their morphology into two major subtypes. Endometrioid carcinomas (type I) are generally estrogen dependent, well-differentiated, superficially invasive, and have a good outcome. Serous carcinomas (type II) are hormone independent, frequently deeply invasive and widely metastatic, and have a poor prognosis. Microarray technology and analysis allows us to determine if the global gene expression profiles of these two subtypes correlate with their morphologic phenotype. Fresh tissue from 18 endometrial carcinomas was studied: 7 well-, 2 moderately, and one poorly differentiated endometrioid, 4 serous carcinomas, and 4 high-grade mixed endometrioid-serous carcinomas. Labeled cDNA probes were synthesized (Cy5 for tumor, Cy3 for reference) and applied to microarrays containing 18,098 cDNA clones or ESTs. A pool of equal amounts of total RNA from each tumor served as the reference RNA. By unsupervised cluster analysis, the endometrioid carcinomas clustered together and were separate from the serous carcinomas. The high-grade mixed carcinomas clustered with the serous carcinomas. Using a statistical algorithm based on gene expression pattern and conducting a supervised analysis of the two defined groups, we have identified 315 genes that statistically differentiate type I from type II endometrial carcinomas. In addition to corroborating the predicted overexpression of known markers (e.g., ras and catenin in endometrioid carcinomas), the cDNA microarray technique has revealed novel alterations in gene expression relevant to cell cycle, cell adhesion, signal transduction, apoptosis, and tumor progression not previously implicated in endometrial carcinomas. For serous carcinomas, these include aldolase, desmoplakin, integrin-linked kinase, PKC, and metallopeptidase. In conclusion, the gene expression profiles of type I and type II endometrial carcinomas are different. Refinement of these profiles will permit more accurate diagnostic tumor classification and the development of prognosis assays.     

923份住院病历抗菌药使用情况调查分析

目的：调查住院患者抗菌药使用情况，提高临床合理使用抗菌药水平。方法：抽取深圳市属某综合医院2004年4月1、4、7、10、13、16、19、22、25、28日共10d34个临床科室的所有出院病历923份，对其抗菌药使用情况进行统计分析。结果：75．1％的患者(693／923)使用了一共86种抗菌药(其中头孢菌素类27种)，其中514例患者使用了2种以上的抗菌药，34例患者使用了6种以上的抗菌药，最多者使用了17种抗菌药。手术科室抗菌药使用率和联合用药率均明显高于非手术科室。仅87例进行了微生物学检查和药敏实验。没有一例进行血药浓度监测。结论：抗菌药使用广泛，比例偏高；多凭经验用药，缺少药敏实验依据。用药方案不尽合理，存在滥用现象。     

萘普生栓治疗产后宫缩痛临床观察

目的了解萘普生栓(NaproxenSuppositories)治疗产后宫缩痛的效果。方法对285例产后宫缩痛的产妇随机分成两组,实验组用萘普生一枚(0﹒25g)塞入肛门内2cm。对照组口服去痛片2片,记录疼痛开始减轻时间,镇痛效果、镇痛持续时间。结果①用药后15分钟疼痛开始减轻者:实验组80例,占55.17﹪;对照组0例。②疼痛缓解者:实验组108例,占74.5﹪。对照组60例,占42.8﹪。③镇痛时间超过8小时者:实验组110例,占79.1﹪。对照组20例,占16.7﹪。结论萘普生栓治疗产后宫缩痛高效、快速、持久。     

手术治疗中晚期胃癌126例报告

目的研究分析胃癌围手术期处理及并发症预防。方法本组126例,男84例,女42例,年龄34岁￣79岁,平均64岁,术前行特殊准备,纠正贫血、低蛋白血症,进行CRIS分级及FEV1测定。选择时机行胃空肠Roux-en-Y吻合手术治疗。结果临床治愈123人,死亡3人,并发症:切口感染8例,切口裂开6例,心肌梗死1例,十二指肠残端瘘1例,吻合口瘘2例,肺炎1例,并发症发生率15.0(%19/126)。结论①术前充分纠正贫血、低蛋白血症、水电解质紊乱,对心肺疾病者行CRIS分级评定和FEV1测定是保证手术成功的要点。②手术原则上行根治性切除,减少瘤负荷,以Roux-en-Y术式为佳。③术后输入端充分减压,输出端适时肠内营养,并严格心肺功能监测,合理应用抗生素,可望降低死亡率。     

Immune responses against SARS-coronavirus nucleocapsid protein induced by DNA vaccine

The nucleocapsid （N） protein of SARS-coronavirus （SARS-CoV） is the key protein for the formation of the helical nucleocapsid during virion assembly. This protein is believed to be more conserved than other proteins of the virus, such as spike and membrane glycoprotein. In this study, the N protein of SARS-CoV was expressed in Escherichia coli DHSalpha and identified with pooled sera from patients in the convalescence phase of SARS. A plasmid pCI-N, encoding the full-length N gene of SARS-CoV, was constructed. Expression of the N protein was observed in COS1 cells following transfection with pCI-N. The immune responses induced by intramuscular immunization with pCI-N were evaluated in a murine model. Serum anti-N immunoglobutins and splenocytes proliferative responses against N protein were observed in immunized BALB/c mice. The major immunoglobulin G subclass recognizing N protein was immunoglobulin G2a, and stimulated splenocytes secreted high levels of gamma interferon and IL-2 in response to N protein. More importantly, the immunized mice produced strong delayed-type hypersensitivity （DTH） and CD^8＋ CTL responses to N protein.