塞来昔布对体外培养软骨细胞活性及细胞外基质合成影响的实验研究

目的:通过细胞学实验来研究塞来昔布对体外培养软骨细胞活性及细胞外基质合成的影响.方法:用酶消化法分离兔关节软骨细胞,利用相差显微镜和H.E.染色软骨细胞形态学观察,Ⅱ型胶原免疫细胞化学染色,Western blot检测金属蛋白酶(MMP-1)、MMP-3蛋白,阿利新蓝法检测糖氨多糖(GAG)的合成.结果:随传代次数增加,细胞逐渐变大,72 h后见细胞大部分贴壁并延伸呈成纤维细胞形态.5 W时A组、B组、C组的阳性免疫染色的灰度值A、C组灰度弱于B组(P＜0.05),A、C组间差异无统计学意义(P＞0.05).在3、5 w时C组软骨细胞较B组软骨细胞的MMP-1、3蛋白表达量下降,P＜0.01,A组在各周无明显变化.3 W与5 W时A、C组糖氨多糖含量高于B组(P＜0.01).结论:塞来昔布可以抑制IL-1所产生的不良效应;可通过减少MMPs的合成,保护软骨基质的降解来维持细胞活性与细胞外基质的合成.     

Successful autologous bone marrow transplant for relapsed Ki-1 lymphoma

We report on a 16 year old girl with relapsed Ki-1 lymphoma and a very poor prognosis. The initial manifestation was multiple bone metastases and lymphadenopathy. The patient achieved remission with modified adriamycin, bleomycin, vincristine, daunomycine therapy. However, 14 months after the completion of therapy, relapse occurred in a new cervical lymph node on the left side. After preparation with chemotherapy and total lymphoid irradiation (TLI) the patient underwent autologous bone marrow transplantation (A-BMT). Ki-1 lymphoma shows clinically diverse symptoms, but hematopoietic stem cell transplantation should be performed in relapsed cases. It may be effective to give TLI followed by A-BMT for patients such as ours who have lymph node involvement without bone marrow metastasis.     

4-吡啶甲醛及其衍生物的新合成法

本文报告一个简便的合成4-吡啶甲醛的方法.4-甲基吡啶在硫黄与碳酸氢钠存在下,与盐酸氨基脲缩合,生成4-吡啶甲醛缩氨基脲.后者在稀盐酸中与二氧化硫作用转变成4-吡啶甲醛亚硫酸加成物和盐酸氨基脲.4-吡啶甲醛的总产率按4-吡啶甲醛亚硫酸加成物计算约47%.     

Regional localization of polymorphic markers on chromosome 10 by physical and genetic mapping

The human vimentin gene and a random DNA segment (D10S39) were mapped to the short arm of human chromosome 10 by linkage analysis. A panel of somatic cell hybrids and monosomy cell-lines, which divide chromosome 10 into seven regions, was used to localize 10 polymorphic markers on this chromosome. The physical map locations obtained correlate well with linkage maps of chromosome 10. Two markers which have been shown to be closely linked to the gene for multiple endocrine neoplasia type 2A map distal to a translocation breakpoint in band 10qll.2     

BLOOD LOSS IN TOTAL HIP REPLACEMENT: EXTRADURAL v. PHENOPERIDINE ANALGESIA

The effects of phenopendine and extradural analgesia on bloodloss during and after total hip replacement were compared in41 patients randomly divided into two statistically comparablegroups. Mean blood loss in patients who received phenoperidinewas 1065 ± 316ml and in patients who received extraduralanalgesia with 0 5% bupivacaine with adrenaline 1:200 000 itwas 650 ±277 ml (P <0 001). There was no significantdifference in postoperative blood loss between the two groupsThe reduction in blood loss resulting from the extradural blockmay prove beneficial in decreasing the hazard and cost of bloodtransfusions and in facilitating autologous transfusion.