The role of the Child Health Services in the identification of children with possible Attention Deficit Hyperactivity Disorder/Deficits in Attention, Motor Control and Perception (ADHD/DAMP)

Literature concerning the role of the Child Health Services in the identification of children with possible Attention Deficit Hyperactivity Disorder/Deficits in Attention, Motor Control and Perception (ADHD/DAMP) is summarized in order to establish a background for evaluation, discussion and conclusion.     

Nutritional and genetic contributions to serum cholesterol concentration in a children's follow-up study

In the Finnish multicentre study of cardiovascular risk in young Finns in 1980, 1983 and 1986, 2429, 2052 and 1841 9 to 18-year-old children and adolescents participated. In 1980, subjects of eastern origin living in the west had, despite their western diet, higher serum cholesterol concentrations than subjects both residing in and originating from the west. In males, eastern origin increased the eastlwest difference in serum cholesterol concentrations. Between 1980 and 1986 the mean serum total cholesterol of the study cohorts decreased by 5.5% and simultaneously the east/west differences in serum cholesterol concentrations disappeared in boys. The study suggests that genetic background is a separate factor determining serum total cholesterol level, but with declining serum cholesterol concentrations the effect of the genetic factor does not become manifest.     

Bcl-2 and Bcl-xL in Peroxide-Resistant A549 and U87MG Cells

Overexpression of the bcl-2 and the related bcl-x_L protoonco-geneproteins enhance cell survival by inhibiting apoptosis inducedby many agents including oxidants. Whether these proteins contributeto survival in oxidant-resistant cells is not known. The currentstudy assessed the expression of bcl-2 and bcl-x_L proteins inhuman glioblastoma U87MG cells and human lung adenocar-cinomaA549 cells selected for resistance to 0, 50, 100, 200, and 400µM H₂O₂ by exposure to this oxidant one time each passagefor 9 months. When examined 7 to 32 days after cessation ofperoxide exposure (times when peroxide resistance was maintained),bcl-2 protein levels were significantly increased in most peroxide-resistantU87MG cells. However, the increase was not dose dependent andwas not accompanied by an increase in mRNA levels. A549 cellsdid not express significant levels of bcl-2 protein, althoughbcl-2 mRNA was detectable. A549 cells expressed large amountsof bcl-x_L and immunohistochemistry demonstrated extensive localizationof this protein around the nucleus. However, expression of thisprotein was not altered in peroxide-resistant lines nor wasbcl-2 protein increased to a measurable level. U87MG cells alsoexpressed bcl-x_L but it was not altered in peroxide-resistantcells. Although the increased bcl-2 protein in peroxide-resistantU87MG cells may contribute to their oxidant tolerance, the lackof a dose-response relationship, the failure to induce bcl-x_Lprotein, and the absence of any bcl-2 or bcl-x_L protein inductionin peroxide-resistant A549 cells suggest these genes are notprimary factors in oxidant resistance.     

中国和英国精神科医生对儿童多动行为症状的评估比较

目的 :比较不同文化背景的精神专科医生在评估多动症儿童各种症状表现时的一致程度 ,探讨文化对儿童多动症诊断可能产生的影响。方法 :使用单独或集体行为观察清单 ,让两地的精神科医生对儿童多动症的各种表现进行症状评估 ;所有的评估者均观察同一录像带上患儿的行为表现 ;对其评定结果进行统计和比较。结果 :儿童独自活动观察量表的 12项中有 11项症状条目评定结果类似 ,有 3项英国医生的评分高于中国医生 ,有 9项中国医生评分高于英国医生 ,其中 1项具有显著性差异。结论 :对行为表现和症状认知的文化差异可能使流行病学的研究出现不同的结果 ,即使使用统一的诊断标准和症状评分量表作为诊断工具 ,不同文化背景的国家 ,儿童多动症的患病率仍然可不一致     

Colorectal liver metastases: Current management and future perspectives

The liver is the commonest site of metastatic disease for patients with colorectal cancer, with at least 25% developing colorectal liver metastases (CRLM) during the course of their illness. The management of CRLM has evolved into a complex field requiring input from experienced members of a multi-disciplinary team involving radiology (cross sectional, nuclear medicine and interventional), Oncology, Liver surgery, Colorectal surgery, and Histopathology. Patient management is based on assessment of sophisticated clinical, radiological and biomarker information. Despite incomplete evidence in this very heterogeneous patient group, maximising resection of CRLM using all available techniques remains a key objective and provides the best chance of long-term survival and cure. To this end, liver resection is maximised by the use of downsizing chemotherapy, optimisation of liver remnant by portal vein embolization, associating liver partition and portal vein ligation for staged hepatectomy, and combining resection with ablation, in the context of improvements in the functional assessment of the future remnant liver. Liver resection may safely be carried out laparoscopically or open, and synchronously with, or before, colorectal surgery in selected patients. For unresectable patients, treatment options including systemic chemotherapy, targeted biological agents, intra-arterial infusion or bead delivered chemotherapy, tumour ablation, stereotactic radiotherapy, and selective internal radiotherapy contribute to improve survival and may convert initially unresectable patients to operability. Currently evolving areas include biomarker characterisation of tumours, the development of novel systemic agents targeting specific oncogenic pathways, and the potential re-emergence of radical surgical options such as liver transplantation.     

重组人BAFF112-285的制备及活性分析

目的 制备具有功能活性的重组人TNF家族的B细胞激活因子(B cell activating factor belonging to the TNF family,BAFF)胞外区112—285氨基酸残基段(rhBAFF112—285)，为BAFF的深人研究奠定基础。方法 提取人HL-60细胞总RNA，经RT—PCR扩增编码人BAFF胞外区112—285氨基酸残基(BAFF112—285)的cDNA，行序列测定后，构建于原核表达载体pQE—80L并转化大肠杆菌DH5α，经IPTG诱导表达rhBAFF112—285，Ni^2 —NTA层析纯化，进而经^3H—TdR掺人实验检测其免疫学活性。结果 RT—PC双扩增得到了525bp的DNA片段，序列分析与GenBank中报道的编码人BAFF112—285的cDNA序列一致，该胞外区片段在大肠杆菌中获得了高效表达，表达水平达细菌总蛋白的45．7％，经纯化后纯度可达98．4％，活性检测证实其能明显刺激外周血淋巴细胞活化。结论 利用大肠杆菌可高效表达rhBAFF112—285，所获纯化产物具有生物学活性，所获结果为进一步研究创造了条件。     

Botulinum toxin A in the hemiplegia upper limb: a double-blind trial

In a randomised, double-blind study, the effects of intramuscular injection of botulinum toxin type A (BtA) into the upper limb were compared with those of normal saline solution in 14 patients with cerebral palsy; their mean age was 9 years. Range of movement and function were assessed before injection and at 2 and 12 weeks after injection. BtA injection significantly increased maximum active elbow and thumb extension and significantly reduced tone at wrist and elbow. The hand grasp-and-release score improved, representing a modest functional change, but fine motor function, assessed by the ability to pick up coins, did not improve and in some cases deteriorated temporarily. The most notable subjective change was the cosmetic benefit of reduced involuntary elbow flexion. The tone-reducing effect of BtA was clinically detectable in comparison with the placebo and patients and parents perceived the change as beneficial. The median of changes in the treatment group was small but the range Was large, suggesting that BtA can be useful in selected patients.     

酸处理对光敏玻璃离子边缘封闭性的影响

研究酸蚀对光敏玻璃离子的微渗漏影响。方法：采用侵害性窝沟封闭技术处理36颗人离休磨牙，并随机分成3组，每组12颗，第Ⅰ组为对照组，用37％磷酸酸蚀后，用低粘稠度复合树脂Liquicoat封闭窝沟裂隙。第Ⅱ组处理同第Ⅰ组，用低粘稠度光敏玻璃离子PrimaFlow封闭窝沟裂隙。第Ⅲ组与第Ⅱ组不同之处，对牙釉质不进行酸蚀。每组半数牙齿进行热循环处理（5～55℃，2500周）。所有标本用0．5％亚甲基蓝着色，在立体显微镜下观察微渗漏等级。结果：微渗漏第Ⅰ组与第Ⅱ组相似（P＞0．05）。第Ⅰ、Ⅱ组较第Ⅲ组小（P＜0．001）。复合树脂经热循环处理后微渗漏有增加（P＜0．01），而光敏玻璃离子粘固粉经热循环处理后微渗漏没有增加（P＞0．05）。结论：光敏玻璃离子结合酸蚀技术能减少微渗漏。     

Compression of radiological images with 512, 1,024, and 2,048 matrices

A comprehensive study was performed using the full-frame bit-allocation compression technique on 78 radiological images, including digitized radiographs, computed radiographs, and computed tomography images. Each radiograph was digitized to 2,048 X 2,048 X 10, 1,024 X 1,024 X 10, and 512 X 512 X 10 matrices, respectively, with a laser scanner. Five compression ratios were used to compress each image, and reconstructed images from each compressed data set were obtained. Altogether, the authors studied 842 images, including the original and compressed-reconstructed images and the images obtained from the difference between the original and the reconstructed images. The results indicate that acceptable compression ratios for 2,048, 1,024, and 512 matrices are 25:1, 20:1, and 10:1, respectively, based on a mean-square error of 0.02%.