Characterization of lymphoid cell populations in bovine lymphosarcomas (“leukosis”) by flow-cytofluorometry

Acridine orange stained cell suspensions from ten cases of bovine lymphosarcoma were analyzed by flow-cytofluorometry. The green DNA-AO fluorescence showed in all cases, but one, two distinct separate lymphoid cell subpopulations. One corresponded to (normal) diploid lymphoid cells, the other had variable aneuploid values. The histograms of the latter cell populations also indicated cells in S- and G₂-phase. Low-angle light scatter histograms did not show a distinct separation between the different cell types, although two maxima could be distinguished. The relative proportions between the two cell populations varied between 20 and 80% with domination of one or the other cell type.There was some conformity to the histological and cytological picture in the ordinary light microscope, although neither a clear distinction in separable cell types nor differential counts was possible.The diploid lymphocyte subpopulation in the tumor might represent an immunoreactive process against the BLV-induced lymphosarcoma.     

活血止痛方的抗炎及抗血栓形成作用

目的 :观察活血止痛方对小鼠的抗炎作用及对大鼠血栓形成的影响。方法 :用二甲苯作为致炎剂 ,观察活血止痛方对二甲苯所致小鼠耳廓肿胀的影响 ,采用大鼠体外颈总动脉—颈外静脉血流旁路形成血小板血栓法 ,观察活血止痛方的抗血栓形成作用。结果 :活血止痛方能显著抑制耳肿胀 (P<0 .0 1) ,能显著减轻血栓湿重 (P<0 .0 1)。结论 :活血止痛方有明显的抗炎、抗脑血栓形成作用     

三黄软膏皮肤用药毒性实验研究

为观察三黄软膏药物外用的安全性 ,进行了动物完整皮肤及破损皮肤长期接触三黄软膏 ,经皮肤渗透对局部及全身产生的毒性和反应的恢复程度 ,用三黄软膏 2g·kg-1、10g·kg-1剂量 (分别相当于 6 0kg人临床用量的 10、5 0倍 )连续对家用兔外用给药4周及停药恢复性观察 2周。结果表明给药期动物的皮肤状况、全身症状、行为活动、摄食、粪便性状、体重增长、血液学均未见明显的毒性反应 ,而高剂量组动物血液生化指标AST、ALT明显增高 ,病理组织学检查时肝水肿明显 ,可见有一定的毒性反应 ;停药期动物的血液生化指标AST、ALT肝组织病理组织学检查恢复正常 ,其他各项指标均未见明显的毒性反应 ,可见该毒性反应是可逆恢复的。表明三黄软膏在临床用药范围内连续使用是比较安全的。     

突发性聋患者血浆一氧化氮和内皮素含量的测定

目的 :探讨血浆一氧化氮 (NO)和内皮素 (ET)水平在突发性聋 (突聋 )发病过程中的作用。方法 :对32例突聋患者用硝酸还原酶法测定血浆NO ,用放射免疫法测定血浆ET。结果 :突聋组血浆NO显著降低 ,ET显著升高 ,与正常对照组相比较 ,突聋组血浆NO显著降低 (P <0 .0 1) ,ET显著升高 (P <0 .0 1) ;突聋治疗前与治疗后血浆NO、ET之间差异有显著性意义 (P <0 .0 1) ;治疗有效者与无效者血清NO、ET水平的差异也有显著性意义 (P <0 .0 1)。结论 :在突聋发作过程中 ,血浆NO和ET的含量显著变化 ,可能促进了疾病的发生和发展     

地塞米松体外诱导宫颈癌细胞株凋亡及其机制的研究

目的 :研究地塞米松 (Dex)体外能否诱导宫颈癌细胞株 (HeLa细胞 )发生凋亡及其机制。方法 :采用透射电镜、DNALadder、流式细胞仪等方法进行凋亡检测 ,用ECLWesternblot方法检测凋亡过程中相关基因表达的变化。结果 :地塞米松体外作用于HeLa细胞后 ,透射电镜观察到了凋亡小体 ;DNALadder法也检测到了凋亡时DNA降解形成的梯带 ;流式细胞仪检测到了细胞凋亡峰 ,且峰值随着处理时间的延长而增高。在凋亡过程中 ,凋亡相关基因bcl 2表达下调 ,而p5 3基因表达无明显改变。结论 :地塞米松体外能诱导HeLa细胞发生凋亡 ,其机制与bcl 2基因的表达下调有关     

HBV-YMDD变异患者与中医虚实辨证关系初探

目的 :初步探讨服用拉米夫定后HBV -YMDD变异患者中医辨证虚证、实证之间的关系。方法 :对服用拉米夫定半年以上的乙型慢性病毒性肝炎患者采用联合基因集团上海博华基因技术有限公司出品的迈科锐乙型肝炎病毒突变检测基因芯片 ,进行HBV -YMDD变异检测。出现HBV -YMDD者 ,根据中医基础理论 ,将其分为实证、虚证 ,来探讨HBV -YMDD变异患者中医虚、实辨证的关系。结果 :HBV -YMDD变异患者中实证变异率(38/ 4 8)明显高于虚证 (10 / 4 8) ,具有统计学意义。结论 :HBV -YMDD变异与虚、实辨证之间具有相关性。     

咳尔康口服液体外抑菌作用的研究

Objective To analyze the effect of Kecrkang oral Liquid on extracorporeal bacteriostasis. Methods Tube dilution method was adopted to test the effect of different concentrations of Keerkang oral liquid on bacillus coli, klebsiella pneumoniae, bacillus aeruginosus, staphylococcus aureus, staphylococcus epidermidis, β-Streptococcus hemolyticus, streptococcus pneumoniae, haemophilus influenzae and candida albicans. Results Keerkang Oral Liquid has obvious inhibited effects to all the bacteria that mentioned above. Conclusion Keerkang Oral Liquid has obvious extracorporeal bacteriostasis and resistance to fungi.     

Patient Positioning Using Magnetic Implants and Magnetic Field Sensors

A phantom study was conducted in order to evaluate the precision and reproducibility of a magnetic field positioning system using magnetic implants and magnetic field sensors. Three implants and three magnetic field sensors were used to position a phantom on a Simulix Y simulator; x-ray films were used as reference. The reproducibility of the position determination of the three magnetic field sensors was checked three times in five different positions in both the X and Y dimensions of the sensors. The phantom was then positioned by the system five times and the displacement errors of four markers in the periphery of the phantom were noted. A reference x-ray film was exposed before the positioning procedure. An error of one millimetre was permitted in each dimension in one sensor. Only the maximum displacement errors of one of the four markers were noted. In the first reproducibility test the system was found to miss 1 mm in one reading out of 90 position readings. In the second part, the mean for the maximum displacement error was 0.46 and 0.6 mm for the maximum frontal and lateral displacements respectively, with a maximal error of 1.3 mm.     

钢基Cu-5%Al激光熔覆层颗粒组织分布的定量分析

熔覆层中的颗粒相分布不仅影响材料性能，同时也是研究熔覆过程各种反应机制的主要依据。本文利用图像分析技术，定量分析了Ｑ２３５钢表面Ｃｕ５％Ａｌ激光熔覆层的富铁相颗粒分布情况。发现熔覆过程中在熔池中存在熔体对流环，熔池中的对流带动颗粒运动，是决定熔覆层颗粒分布的主要因素。