全文获取类型
收费全文 | 4407篇 |
免费 | 299篇 |
国内免费 | 16篇 |
专业分类
耳鼻咽喉 | 66篇 |
儿科学 | 173篇 |
妇产科学 | 102篇 |
基础医学 | 724篇 |
口腔科学 | 109篇 |
临床医学 | 316篇 |
内科学 | 1148篇 |
皮肤病学 | 120篇 |
神经病学 | 367篇 |
特种医学 | 114篇 |
外科学 | 660篇 |
综合类 | 15篇 |
一般理论 | 3篇 |
预防医学 | 216篇 |
眼科学 | 105篇 |
药学 | 242篇 |
中国医学 | 11篇 |
肿瘤学 | 231篇 |
出版年
2023年 | 20篇 |
2022年 | 50篇 |
2021年 | 120篇 |
2020年 | 60篇 |
2019年 | 125篇 |
2018年 | 136篇 |
2017年 | 78篇 |
2016年 | 89篇 |
2015年 | 105篇 |
2014年 | 134篇 |
2013年 | 175篇 |
2012年 | 285篇 |
2011年 | 295篇 |
2010年 | 157篇 |
2009年 | 154篇 |
2008年 | 242篇 |
2007年 | 245篇 |
2006年 | 235篇 |
2005年 | 239篇 |
2004年 | 238篇 |
2003年 | 227篇 |
2002年 | 196篇 |
2001年 | 182篇 |
2000年 | 196篇 |
1999年 | 136篇 |
1998年 | 53篇 |
1997年 | 41篇 |
1996年 | 39篇 |
1995年 | 23篇 |
1994年 | 21篇 |
1993年 | 19篇 |
1992年 | 66篇 |
1991年 | 45篇 |
1990年 | 41篇 |
1989年 | 27篇 |
1988年 | 25篇 |
1987年 | 35篇 |
1986年 | 14篇 |
1985年 | 15篇 |
1984年 | 17篇 |
1983年 | 17篇 |
1982年 | 9篇 |
1979年 | 11篇 |
1977年 | 9篇 |
1976年 | 6篇 |
1975年 | 8篇 |
1973年 | 6篇 |
1971年 | 6篇 |
1970年 | 7篇 |
1968年 | 6篇 |
排序方式: 共有4722条查询结果,搜索用时 15 毫秒
121.
Archundia García A Gómez Alvarez E Ixcamparij Rosales C Blanco Canto M Hernández Ruiz MA 《Archivos del Instituto de Cardiología de México》2000,70(5):492-498
This is the diagnostic experiences as well as the surgical mode of treatment used in a 31 years old women suffering diversion of the inferior vena cava into the left atrium associated with atrial septal defect. The patient had been previously studied and operated thrice under conventional circumstances at different institutions in order to solve the septal defect. The hemodynamic solution had not been reached due to a missing pathological definition. The cineangiogram through the saphenous vein specified the left atrium form the inferior vena cava. Some considerations are made on the surgical methods used for the fourth operation, and in regard of the fact that the patient refused blood transfusion because of religious convictions (Jehova Witness). 相似文献
122.
Emily R. Watts Andrew J.M. Howden Tyler Morrison Pranvera Sadiku Jens Hukelmann Alex von Kriegsheim Bart Ghesquiere Fiona Murphy Ananda S. Mirchandani Duncan C. Humphries Robert Grecian Eilise M. Ryan Patricia Coelho Gio Rodriguez Blanco Tracie M. Plant Rebecca S. Dickinson Andy Finch Wesley Vermaelen Doreen A. Cantrell Moira K. Whyte Sarah R. Walmsley 《The Journal of clinical investigation》2021,131(10)
Limiting dysfunctional neutrophilic inflammation while preserving effective immunity requires a better understanding of the processes that dictate neutrophil function in the tissues. Quantitative mass-spectrometry identified how inflammatory murine neutrophils regulated expression of cell surface receptors, signal transduction networks, and metabolic machinery to shape neutrophil phenotypes in response to hypoxia. Through the tracing of labeled amino acids into metabolic enzymes, proinflammatory mediators, and granule proteins, we demonstrated that ongoing protein synthesis shapes the neutrophil proteome. To maintain energy supplies in the tissues, neutrophils consumed extracellular proteins to fuel central carbon metabolism. The physiological stresses of hypoxia and hypoglycemia, characteristic of inflamed tissues, promoted this extracellular protein scavenging with activation of the lysosomal compartment, further driving exploitation of the protein-rich inflammatory milieu. This study provides a comprehensive map of neutrophil proteomes, analysis of which has led to the identification of active catabolic and anabolic pathways that enable neutrophils to sustain synthetic and effector functions in the tissues. 相似文献
123.
Pedro David Delgado López Beatriz Blanco de Val José Luis López Martínez Elena Araus Galdós Antonio Rodríguez Salazar 《Neurocirugía (Asturias, Spain)》2021,32(2):99-104
It is an increasingly common practice to indicate a carotid endarterectomy procedure based on the information provided by non-invasive tests like Duplex ultrasound, MR angiography or CT angiography, thereby obviating the performance of a conventional cerebral angiography. We present a case of symptomatic left carotid artery 80% stenosis in which cerebral angiography showed absence of the right A1 segment and bilateral anterior cerebral artery territories that filled only from a left injection. Just 90 seconds after carotid artery clamping at the neck, brain oximetry and somatosensory evoked potentials significantly dropped, that recovered after immediate clamp removal. Endarterectomy was dismissed and a carotid stent was successfully placed. This case highlights the importance of knowing the dynamics of cerebral blood circulation distal to the stenosis. If endarterectomy had been attempted, unawareness of the information provided by the cerebral angiography would have likely result in severe bi-hemispheric ischemia. 相似文献
124.
125.
Cytomegalovirus prevention strategies in seropositive kidney transplant recipients: an insight into current clinical practice
下载免费PDF全文
![点击此处可从《Transplant international》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Mario Fernández‐Ruiz Manuel Arias Josep M. Campistol David Navarro Ernesto Gómez‐Huertas Gonzalo Gómez‐Márquez Juan Manuel Díaz Domingo Hernández Gabriel Bernal‐Blanco Frederic Cofan Luisa Jimeno Antonio Franco‐Esteve Esther González Francesc J. Moreso Carlos Gómez‐Alamillo Alicia Mendiluce Enrique Luna‐Huerta José María Aguado the OPERA Study Group 《Transplant international》2015,28(9):1042-1054
There is notable heterogeneity in the implementation of cytomegalovirus (CMV) prevention practices among CMV‐seropositive (R+) kidney transplant (KT) recipients. In this prospective observational study, we included 387 CMV R+ KT recipients from 25 Spanish centers. Prevention strategies (antiviral prophylaxis or preemptive therapy) were applied according to institutional protocols at each site. The impact on the 12‐month incidence of CMV disease was assessed by Cox regression. Asymptomatic CMV infection, acute rejection, graft function, non‐CMV infection, graft loss, and all‐cause mortality were also analyzed (secondary outcomes). Models were adjusted for a propensity score (PS) analysis for receiving antiviral prophylaxis. Overall, 190 patients (49.1%) received preemptive therapy, 185 (47.8%) antiviral prophylaxis, and 12 (3.1%) no specific intervention. Twelve‐month cumulative incidences of CMV disease and asymptomatic infection were 3.6% and 39.3%, respectively. Patients on prophylaxis had lower incidence of CMV disease [PS‐adjusted HR (aHR): 0.10; 95% confidence interval (CI): 0.01–0.79] and asymptomatic infection (aHR: 0.46; 95% CI: 0.29–0.72) than those managed preemptively, with no significant differences according to the duration of prophylaxis. All cases of CMV disease in the prophylaxis group occurred after prophylaxis discontinuation. There were no differences in any of the secondary outcomes. In conclusion, antiviral prophylaxis was associated with a lower occurrence of CMV disease in CMV R+ KT recipients, although such benefit should be balanced with the risk of late‐onset disease. 相似文献
126.
Conservation Genetics Resources - We obtained molecular markers useful for population level studies of the Red-billed chough by screening partial genomic DNA libraries enriched for microsatellite... 相似文献
127.
The alpha subunit of the Na,K-ATPase specifically and stably associates into oligomers. 总被引:1,自引:0,他引:1
下载免费PDF全文
![点击此处可从《Proceedings of the National Academy of Sciences of the United States of America》网站下载免费的PDF全文](/ch/ext_images/free.gif)
G Blanco J C Koster R W Mercer 《Proceedings of the National Academy of Sciences of the United States of America》1994,91(18):8542-8546
The Na,K-ATPase is a heterodimer consisting of an alpha and a beta subunit. Both Na,K-ATPase subunits are encoded by multigene families. Several isoforms for the alpha (alpha 1, alpha 2, and alpha 3) and beta (beta 1, beta 2, and beta 3) subunits have been identified. All these isoforms are capable of forming functionally active enzyme. Although there is general agreement that the Na,K-ATPase consists of alpha and beta subunits in equimolar amounts, the quaternary structure of the Na,K-ATPase and its functional significance is unknown. Several studies have demonstrated that the enzyme exists within the plasma membrane as an oligomer of alpha beta dimers. However, because the alpha beta protomer seems to be catalytically competent, the possibility exists that higher oligomers are irrelevant to function. The ability to express different alpha isoforms in insect cells and the availability of isoform-specific antibodies has provided the opportunity to test for the existence of stable and specific associations among alpha subunits. By coexpressing different alpha-subunit isoforms in cultured cells, we demonstrate that the Na,K-ATPase alpha subunits specifically and stably associate into oligomeric complexes. This same association among alpha-subunit isoforms was demonstrated in the native enzyme from rat brain. The interaction between Na,K-ATPase alpha subunits is highly specific. When the Na,K-ATPase alpha subunit is coexpressed with the alpha subunit from the H,K-ATPase, the H,K subunit does not associate with the Na,K subunit. Moreover, expression of the truncated alpha 1T isoform with the full-length alpha subunit demonstrates that the C-terminal portion of the polypeptide is important in the alpha-subunit association. Although these results do not clarify the functional role of alpha alpha associations, they do establish their highly specific nature and suggest that oligomerization of alpha beta protomers may be important to the stability and physiological regulation of the enzyme. 相似文献
128.
Celiac disease and autoimmune diseases or systemic disease. Six cases and a review of the literature
Delbrel X Le-Bougeant P Etienne G Blanco P Beylot-Barry M Amouretti M Mercie P Longy-Boursier M 《Annales de médecine interne》2003,154(4):197-204
BACKGROUND: Celiac disease is an autoimmune disorder which may be associated with another autoimmune or systemic disease. OBJECTIVE: To determine the links between autoimmune diseases and celiac disease. PATIENTS AND METHODS: Among 31 patients with a celiac disease, we selected those who had another autoimmune or systemic disease. RESULTS: We report 6 patients with such disease association: 3 with autoimmune thyroiditis including one also with Grave's disease, 2 with systemic lupus erythematosus including one also with insulin-dependent diabetes mellitus, and 1 with temporal arteritis. CONCLUSION: The link between celiac disease and autoimmune thyroiditis or insulin-dependent diabetes mellitus seems to be real but many discrepancies are observed for the other autoimmune diseases. After a literature review, we suggest a summary of effective associations between celiac disease and autoimmune or systemic diseases. 相似文献
129.
Carrie C. Hoefer Adolfo Quiñones-Lombraña Rachael Hageman Blair Javier G. Blanco 《Cardiovascular toxicology》2016,16(2):182-192
The intracardiac synthesis of anthracycline alcohol metabolites by aldo–keto reductases (AKRs) contributes to the pathogenesis of anthracycline-related cardiotoxicity. AKR7A2 is the most abundant anthracycline reductase in hearts from donors with and without Down syndrome (DS), and its expression varies between individuals (≈tenfold). We investigated whether DNA methylation impacts AKR7A2 expression in hearts from donors with (n = 11) and without DS (n = 30). Linear models were used to test for associations between methylation status and cardiac AKR7A2 expression. In hearts from donors without DS, DNA methylation status at CpG site ?865 correlated with AKR7A2 mRNA (Pearson’s regression coefficient, r = ?0.4051, P = 0.0264) and AKR7A2 protein expression (r = ?0.5818, P = 0.0071). In heart tissue from donors with DS, DNA methylation status at CpG site ?232 correlated with AKR7A2 protein expression (r = 0.8659, P = 0.0025). Multiple linear regression modeling revealed that methylation at several CpG sites is associated with the synthesis of cardiotoxic daunorubicinol. AKR7A2 methylation status in lymphoblastoid cell lines from donors with and without DS was examined to explore potential parallelisms between cardiac tissue and lymphoid cells. These results suggest that DNA methylation impacts AKR7A2 expression and the synthesis of cardiotoxic daunorubicinol. 相似文献
130.
Delia Blanco Esther Perez-Herran Mónica Cacho Lluís Ballell Julia Castro Rubén González del Río José Luis Lavandera Modesto J. Remui?án Cindy Richards Joaquin Rullas María Jesús Vázquez-Mu?iz Ermias Woldu María Cleofé Zapatero-González I?igo Angulo-Barturen Alfonso Mendoza David Barros 《Antimicrobial agents and chemotherapy》2015,59(4):1868-1875
One way to speed up the TB drug discovery process is to search for antitubercular activity among compound series that already possess some of the key properties needed in anti-infective drug discovery, such as whole-cell activity and oral absorption. Here, we present MGIs, a new series of Mycobacterium tuberculosis gyrase inhibitors, which stem from the long-term efforts GSK has dedicated to the discovery and development of novel bacterial topoisomerase inhibitors (NBTIs). The compounds identified were found to be devoid of fluoroquinolone (FQ) cross-resistance and seem to operate through a mechanism similar to that of the previously described NBTI GSK antibacterial drug candidate. The remarkable in vitro and in vivo antitubercular profiles showed by the hits has prompted us to further advance the MGI project to full lead optimization. 相似文献