全文获取类型
收费全文 | 452篇 |
免费 | 21篇 |
国内免费 | 22篇 |
专业分类
耳鼻咽喉 | 1篇 |
儿科学 | 37篇 |
妇产科学 | 3篇 |
基础医学 | 47篇 |
口腔科学 | 9篇 |
临床医学 | 52篇 |
内科学 | 59篇 |
皮肤病学 | 8篇 |
神经病学 | 4篇 |
特种医学 | 119篇 |
外科学 | 32篇 |
综合类 | 30篇 |
一般理论 | 2篇 |
预防医学 | 12篇 |
眼科学 | 27篇 |
药学 | 26篇 |
肿瘤学 | 27篇 |
出版年
2021年 | 1篇 |
2020年 | 3篇 |
2019年 | 2篇 |
2018年 | 5篇 |
2017年 | 1篇 |
2016年 | 2篇 |
2015年 | 8篇 |
2014年 | 6篇 |
2013年 | 12篇 |
2012年 | 4篇 |
2011年 | 7篇 |
2010年 | 10篇 |
2009年 | 14篇 |
2008年 | 14篇 |
2007年 | 28篇 |
2006年 | 33篇 |
2005年 | 8篇 |
2004年 | 19篇 |
2003年 | 8篇 |
2002年 | 6篇 |
2001年 | 18篇 |
2000年 | 7篇 |
1999年 | 8篇 |
1998年 | 35篇 |
1997年 | 23篇 |
1996年 | 28篇 |
1995年 | 22篇 |
1994年 | 19篇 |
1993年 | 22篇 |
1992年 | 6篇 |
1991年 | 4篇 |
1990年 | 10篇 |
1989年 | 8篇 |
1988年 | 8篇 |
1987年 | 6篇 |
1986年 | 11篇 |
1985年 | 8篇 |
1984年 | 7篇 |
1983年 | 6篇 |
1982年 | 7篇 |
1981年 | 8篇 |
1980年 | 13篇 |
1979年 | 3篇 |
1978年 | 4篇 |
1977年 | 4篇 |
1976年 | 4篇 |
1975年 | 1篇 |
1969年 | 1篇 |
1968年 | 1篇 |
1882年 | 1篇 |
排序方式: 共有495条查询结果,搜索用时 9 毫秒
51.
Alterations in neutrophil subpopulations during human hemodialysis or following injection of C5a des arg into rabbits were studied. Whereas baseline peripheral blood neutrophils contained approximately 80% of cells that formed rosettes with IgG-sensitized erythrocytes, neutrophils harvested at the granulocyte nadir (20 min after initiating hemodialysis or the injection of C5a des arg) were markedly depleted of this population. This was seen in a change in ratio of rosette-forming neutrophils (RFN) to non-rosette-forming neutrophils (non-RFN) from 4:1 at 0 time to 1:2 at 20 min. Since non-RFN are less active in assays of adherence and chemotaxis, these alterations in circulating neutrophil populations were reflected in abnormal functional capacity of neutrophils harvested at 20 min. To study the mechanism of RFN depletion, we investigated the ability of C5a des arg to aggregate various human neutrophil suspensions. Unfractionated neutrophils and RFN demonstrated prompt in vitro aggregation in response to C5a des arg, whereas this activated complement fragment induced little aggregation in a population enriched for non-RFN. These results may explain the alterations in neutrophil adherence, chemotaxis, phagocytosis, and bactericidal activity, which have been reported to accompany clinical disorders characterized by in vivo complement activation (i.e., hemodialysis or gram-negative sepsis). 相似文献
52.
Lina Panayiota Aristoteli BSc Mark DP Willcox PhD 《Clinical & experimental ophthalmology》2001,29(3):143-146
The purpose of this study was to compare the adhesion of Pseudomonas aeruginosa ocular isolates to mucin. An adhesion assay was developed using biotin‐labelled P. aeruginosa strains (two corneal ulcer, two acute red eye, one asymptomatic and one standard strains) incubated with porcine gastric mucin immobilized on a nitrocellulose membrane. The adhesion was semiquantified using densitometry. The results showed that all P. aeruginosa strains tested were able to adhere to mucin to various extents with three strains (one corneal ulcer, one acute red eye, one asymptomatic) binding significantly greater than the negative control (P < 0.1). Results suggest that ocular strains of P. aeruginosa strains differ in their adhesion to mucin but this did not correlate with the pathogenic origin of the strain. It is concluded that the adhesion of P. aeruginosa strains to mucin alone may not be a principal determinant of pathogenesis but may be a contributing factor along with other bacterial virulence traits. 相似文献
53.
Mark DP Willcox 《Clinical & experimental optometry》2019,102(4):350-363
This article summarises research undertaken since 1993 in the Willcox laboratory at the University of New South Wales, Sydney on the tear film, its interactions with contact lenses, and the use of tears as a source of biomarkers for ocular and non‐ocular diseases. The proteome, lipidome and glycome of tears all contribute to important aspects of the tear film, including its structure, its ability to defend the ocular surface against microbes and to help heal ocular surface injuries. The tear film interacts with contact lenses in vivo and interactions between tears and lenses can affect the biocompatibility of lenses, and may be important in mediating discomfort responses during lens wear. Suggestions are made for follow‐up research. 相似文献
54.
<Emphasis Type="Italic">BRCA1</Emphasis> and <Emphasis Type="Italic">BRCA2</Emphasis> mutations in a population-based study of male breast cancer 下载免费PDF全文
Victoria?M?Basham Julian?M?Lipscombe Joanna?M?Ward Simon?A?Gayther Bruce?AJ?Ponder Douglas?F?Easton Paul?DP?PharoahEmail author 《Breast cancer research : BCR》2001,4(1):R2
Background
The contribution of BRCA1 and BRCA2 to the incidence of male breast cancer (MBC) in the United Kingdom is not known, and the importance of these genes in the increased risk of female breast cancer associated with a family history of breast cancer in a male first-degree relative is unclear.Methods
We have carried out a population-based study of 94 MBC cases collected in the UK. We screened genomic DNA for mutations in BRCA1 and BRCA2 and used family history data from these cases to calculate the risk of breast cancer to female relatives of MBC cases. We also estimated the contribution of BRCA1 and BRCA2 to this risk.Results
Nineteen cases (20%) reported a first-degree relative with breast cancer, of whom seven also had an affected second-degree relative. The breast cancer risk in female first-degree relatives was 2.4 times (95% confidence interval [CI] = 1.4–4.0) the risk in the general population. No BRCA1 mutation carriers were identified and five cases were found to carry a mutation in BRCA2. Allowing for a mutation detection sensitivity frequency of 70%, the carrier frequency for BRCA2 mutations was 8% (95% CI = 3–19). All the mutation carriers had a family history of breast, ovarian, prostate or pancreatic cancer. However, BRCA2 accounted for only 15% of the excess familial risk of breast cancer in female first-degree relatives.Conclusion
These data suggest that other genes that confer an increased risk for both female and male breast cancer have yet to be found.55.
CH Reynolds BBSc DP Crewther PhD SG Crewther PhD SJ West BSc 《Clinical & experimental ophthalmology》1998,26(S1):S117-S119
56.
57.
A PIL for every ill? Patient information leaflets (PILs): a review of past, present and future use 总被引:9,自引:0,他引:9
Kenny T; Wilson RG; Purves IN; Clark J Sr; Newton LD; Newton DP; Moseley DV 《Family practice》1998,15(5):471-479
This article reviews the usefulness and importance of written information,
specifically leaflets, being given to patients. Evidence suggesting how
both patient and doctor may benefit from the giving of written information
is reviewed. Identification of good practice relating to the content and
readability of leaflets is discussed. An argument is put forward that the
giving of written information is an under-utilized resource in contributing
to improving patient outcomes but that this may be changing with the
increasing use of patient leaflet databases. The advantages and
disadvantages of computer- generated patient leaflets are discussed and
desirable further areas of research on computer-generated leaflets are
proposed.
相似文献
58.
59.
Clinical experience with an endobronchial implant 总被引:1,自引:0,他引:1
60.