携带HGF基因的减毒沙门氏菌对大鼠胃溃疡愈合及CD34表达的影响

目的:通过对乙酸性胃溃疡大鼠口服携带肝细胞生长因子(HGF)基因的减毒鼠伤寒沙门氏菌(St)后检测胃溃疡愈合情况及局部和CD34蛋白表达的研究,探讨基因药物St-HGF对乙酸性大鼠胃溃疡的治疗作用.方法:建立乙酸性胃溃疡大鼠77只,并随机选出5只,给予携带绿色荧光蛋白(GFP)基因的st后观察GFP在大鼠胃壁中的分布,剩余大鼠随机分为3组(n=24):St-HGF治疗组,St治疗组,NaHCO3对照组;增菌后各组动物于造模第2日起灌胃相应药物,隔日一次,分别于给药后7,14,21 d取胃溃疡组织行HE病理组织学观察,免疫组化法检测CD34蛋白的表达.结果:给药后7 d光镜下溃疡部位黏膜消失,局部有大量坏死组织,各组胃黏膜上皮爬行不明显;给药后14,21 d,St-HGF组黏膜上皮增生较另两组快(P<0.01);给药后7,14,21 d,CD34蛋白在St-HGF组的表达高于另两组(P<0.05),组间有统计学差异.结论:St-HGF可促进实验性大鼠胃溃疡黏膜上皮修复和溃疡面血管增生,加快溃疡愈合.     

Predictive factors for live birth in cryopreservation single embryo transfer cycles

IVF cryopreservation cycles are known to be less successful than fresh cycles. The objective of this study was to investigate what variables, maternal and embryonic, that independently predict the live birth rate in cryopreservation single embryo transfers (SET). A retrospective analysis was performed on 622 cryopreservation SET originating from 371 consecutive patients performing 410 fresh cycles. Maternal and embryonic variables were analysed using logistic regression. The overall pregnancy and live birth rates were 22 and 16% respectively. Blastomere survival rate, number of previous fresh cycles and IVF as fertilization method compared with intracytoplasmic sperm injection (ICSI), were positive predictors of live birth. Number of embryos thawed to obtain one transfer was negatively associated with pregnancy rate but not with live birth rate. No statistical difference in live birth rate was found whether the couple had performed one, two or three failed cryopreservation cycles previously from the same egg retrieval. Knowledge of certain predictors for live birth contributes to the estimation of prognosis in cryopreservation SET, and is important when deciding whether to perform single or double embryo transfer in cryopreservation cycles. However, despite being independently associated with live birth and pregnancy, the predictive value of identified variables was low.     

Cumulative impact of adding frozen-thawed cycles to single versus double fresh embryo transfers

Randomized control trials have shown that single embryo transfer (SET) results in lower live birth rates than double embryo transfer (DET), while observational, retrospective studies find no decrease in overall live birth rate when using a SET policy. The cumulative (fresh transfer followed by frozen - thawed transfers of embryos from the same stimulated cycle) live birth rate after the first and the second stimulated cycle of SET and DET respectively has been analysed. All couples who received their first fresh embryo transfer at Sahlgrenska University Hospital during 2003 and 2004 were included (n = 689). The live birth rates after DET versus SET in the first and second fresh cycles were 29.7 (47/158) versus 23.9% (127/531) and 30.8 (41/133) versus 22.0% (45/205). The cumulative live birth rate per patient after the addition of frozen-thawed embryo transfers were similar: 33.5 (53/158) and 34.8% (185/531) for DET and SET respectively after the first cycle and 32.3 (43/133) versus 32.2% (66/205) after the second cycle. A logistic regression analysis showed no significant correlation for SET or DET with cumulative live birth. Thus, cumulative live birth rates are similar after SET and DET in a routine IVF programme with a majority of SET transfers, although a higher number of frozen-thawed cycles were needed in the SET group.     

Decreased testicular response to acute LH-stimulation and increased intratesticular concentration of oestradiol-17 beta in the abdominal testes in cryptorchid rats

Rats were made cryptorchid, bi- or unilaterally, by cutting gubernaculum testis at birth. At 100 days of age the basal plasma testosterone concentration was smaller in bilateral cryptorchid animals as compared to unilateral cryptorchid and control rats. After acute LH-stimulation plasma testosterone concentration increased in all rats, where the highest concentrations were found in the control group. The amount of testosterone in the testis after a 30 min LH-stimulation was lower in both kinds of cryptorchid testes but higher in the scrotal testis of the unilateral cryptorchid rats, all as compared to control testis. This decreased effect of LH observed in cryptorchid animals indicates an impaired Leydig cell function in cryptorchid testis. The results also show a compensatory hyperfunction of Leydig cells in the scrotal testes of unilateral cryptorchid rats. It was also found that the intratesticular concentration of oestradiol-17 beta was three-fold increased in unilateral cryptorchid testes and even more in bilateral cryptorchid ones.     

医学院校生物科学专业课程体系建设探讨

探讨医学生物科学专业课程构架：课程设置以医学基础知识为奠基,突显最新生命科学领域,注重综合课程,加强医学分子生物学技能训练,为社会培养生命科学领域复合、实用型人才。     

高血压致左室流出道梗阻的彩色多普勒超声诊断价值

目的：应用二维及彩色多普勒超声观察左室流出道的血流动力学变化,无创性评价高血压致左室流出道梗阻。方法：23例由于高血压导致的左室流出道梗阻患者,根据有无＂SAM＂征分为两组,无＂SAM＂征组13例,有＂SAM＂征组10例。排除肥厚型梗阻性心肌病,心肌梗死及心脏瓣膜病变。分别测量两组患者静息状态和激发试验下左室流出道血流速度及主动脉瓣上血流速度。结果：所有患者在激发试验（Valsal-va试验）后左室流出道血流速度均高于静息状态血流速度,有＂SAM＂征组静息状态和激发试验后左室流出道血流速度均高于无＂SAM＂征组,主动脉瓣上血流速度两组患者在静息状态和激发试验后差异无统计学意义。结论：超声心动图能无创、准确诊断左室流出道梗阻,激发试验能有效评价梗阻程度。     

HIE磁共振多序列分度与临床分度的一致性研究

目的探讨新生儿缺氧缺血性脑病（HIE）患儿磁共振多序列分度与临床分度的一致性。方法将经临床确诊为HIE的新生儿95例进行包括DWI及ESWAN序列的MRI多序列扫描,分别比较不同序列组合的MRI分度与临床分度的一致性。结果T1WI组MRI分度与临床分度比较Kappa值为0．32（P〈0．05）,一致性较差;T1WI＋DWI组MRI分度与临床分度比较Kappa值为0．57（P〈0．05）,一致性上升;T1WI＋DWI＋ESWAN组MRI分度与临床分度比较Kappa值为0．79（P〈0．05）,一致性好。结论T1WI联合DWI及ESWAN序列后,明显提高了HIE MRI分度与临床分度的一致性。     

四肢创伤围术期的脂肪栓塞综合征

目的:论述四肢创伤围术期脂肪栓塞综合征的病因、病理、临床表现及防治。方法:查阅关于四肢创伤围术期脂肪栓塞综合征方面的文献,进行总结。结果:脂肪栓塞综合征在四肢多发骨折中应引起临床医师认识和重视。结论:脂肪栓塞综合征防治的关键是防止应激反应发生、低血容量、组织缺氧、骨折早期固定、髓内钉手术进行减压处理及皮质类固醇的应用。