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941.
Barros LF 《Pflügers Archiv : European journal of physiology》1999,437(5):763-770
A fluorometric method that allows repeatable measurement of sugar transport rates and parameters in single living cells is
presented. Intracellular sugar concentrations were estimated in real time from changes in cell volume that occur secondary
to permeation of sugars across the plasma membrane. In turn, the cell volume changes were estimated from variations of intracellular
calcein fluorescence measured by confocal microscopy. Using HeLa cells, the assay allowed reproducible measurement of the
uptake and exit of d-galactose and 3-O-methyl-d-glucose. The rate of zero-trans uptake (i.e. at an intracellular concentration of zero) of galactose at an extracellular
concentration of 200 mM was 0.34±0.05 mM/s (n=8). Apparent V
max and K
m for galactose exit were 0.32±0.05 mM/s (n=9) and 30±7.2 mM (n=9), respectively. The apparent affinity of infinite-trans (i.e. at a very high intracellular concentration) uptake of 3-O-methyl-d-glucose was 3.8±0.47 mM (n=8). Galactose uptake was 93±8% (n=8) inhibited in the presence of 50 μM phloretin, whereas galactose exit was 96±6% (n=5) trans-inhibited by 100 mM 4,6-ethylidine-d-glucose. This technique may help to characterize sugar transport in freshly isolated cells, co-cultures and heterogeneous
cell explants. It may also allow available cell microinjection technology to be used to study the regulation of sugar transporters’
intrinsic activity. In principle, similar approaches might also be applied in functional studies of other transporters for
which non-metabolized substrates are available.
Received: 5 August 1998 / Received after revision: 2 October 1998 / Accepted: 10 November 1998 相似文献
942.
Definition of a region of loss of heterozygosity at chromosome 11q in cervical carcinoma. 总被引:2,自引:0,他引:2
C Mugica-Van Herckenrode J A Rodriguez V Iriarte-Campo A Carracedo F Barros 《Diagnostic molecular pathology》1999,8(2):92-96
Chromosomal deletions at segment 11q23-q24 have been identified in a variety of human epithelial tumors, including cervical carcinoma (CC), indicating the presence in this region of at least a tumor suppressor gene (TSG) involved in the development of these neoplasms. To localize the 11q deletion target more precisely, 54 primary cervical carcinomas were examined for loss of heterozygosity (LOH) using a panel of microsatellite DNA markers mapping to 11p.15 and spanning region 11q23-qter. Nineteen tumors were found to have LOH at chromosome 11q. The highest frequency of LOH was observed at locus APOC-3, located in 11q23.1-q23.2, which was deleted in 42% of the informative cases. In contrast, LOH was infrequent at distal 11q in current series of CC. The smallest common region of loss included APOC-3 and was defined distally by marker D11S925 in region 11q23. The present data strongly suggest that the 11q suppressor gene(s) involved in cervical tumorigenesis is likely to be located at chromosome region 11q22-q23. 相似文献
943.
D.R. da Silva I.C. Gluz J. Kurz G.G. Thomé R. Zancan R.N. Bringhenti P.G. Schaefer M. dos Santos E.J.G. Barros F.V. Veronese 《Brazilian journal of medical and biological research》2016,49(4)
HIV infection has a broad spectrum of renal manifestations. This study examined the clinical and histological manifestations of HIV-associated renal disease, and predictors of renal outcomes. Sixty-one (64% male, mean age 45 years) HIV patients were retrospectively evaluated. Clinical presentation and renal histopathology were assessed, as well as CD4 T-cell count and viral load. The predictive value of histological lesion, baseline CD4 cell count and viral load for end-stage renal disease (ESRD) or death were determined using the Cox regression model. The outcomes of chronic kidney disease (CKD) and ESRD or death were evaluated by baseline CD4 cell count. The percent distribution at initial clinical presentation was non-nephrotic proteinuria (54%), acute kidney injury (28%), nephrotic syndrome (23%), and chronic kidney disease (22%). Focal segmental glomerulosclerosis (28%), mainly the collapsing form (HIVAN), acute interstitial nephritis (AIN) (26%), and immune complex-mediated glomerulonephritis (ICGN) (25%) were the predominant renal histology. Baseline CD4 cell count ≥200 cells/mm3 was a protective factor against CKD (hazard ratio=0.997; 95%CI=0.994-0.999; P=0.012). At last follow-up, 64% of patients with baseline CD4 ≥200 cells/mm3 had eGFR >60 mL·min-1·(1.73 m2)-1 compared to the other 35% of patients who presented with CD4 <200 cells/mm3 (log rank=9.043, P=0.003). In conclusion, the main histological lesion of HIV-associated renal disease was HIVAN, followed by AIN and ICGN. These findings reinforce the need to biopsy HIV patients with kidney impairment and/or proteinuria. Baseline CD4 cell count ≥200 cells/mm3 was associated with better renal function after 2 years of follow-up. 相似文献
944.
J.?C.?PieczarkaEmail author C.?Y.?Nagamachi P.?C.?M.?O’Brien F.?Yang W.?Rens R.?M.?S.?Barros R.?C.?R.?Noronha J.?Rissino E.?H.?C.?de?Oliveira M.?A.?Ferguson-Smith 《Chromosome research》2005,13(4):339-347
The Neotropical Phyllostomidae family is the third largest in the order Chiroptera, with 56 genera and 140 species. Most researchers accept this family as monophyletic but its species are anatomically diverse and complex, leading to disagreement on its systematics and evolutionary relationships. Most of the genera of Phyllostomidae have highly conserved karyotypes but with intense intergeneric variability, which makes any comparative analysis using classical banding difficult. The use of chromosome painting is a modern way of genomic comparison on the cytological level, and will clarify the intense intergenus chromosomal variability in Phyllostomidae. Whole chromosome probes of species were produced as a tool for evolutionary studies in this family from two species from different subfamilies, Phyllostomus hastatus and Carollia brevicauda, which have large morphological and chromosomal differences, and these probes were used in reciprocal chromosome painting. The hybridization of the Phyllostomus probes on the Carollia genome revealed 24 conserved segments, while the Carollia probes on the Phyllostomus genome detected 26 segments. Many chromosome rearrangements have occurred during the divergence of these two genera. The sequence of events suggested a large number of rearrangements during the differentiation of the genera followed by high chromosomal stability within each genus. 相似文献
945.
Jordana Maia Dias Tarcisio Vieira de Brito Diva de Aguiar Magalhães Pammela Weryka da Silva Santos Jalles Arruda Batista Eulina Gabriela do Nascimento Dias Heliana de Barros Fernandes Samara Rodrigues Bonfim Damasceno Renan O. Silva Karoline S. Aragão Marcellus H. L. P. Souza Jand-Venes R. Medeiros André Luiz R. Barbosa 《Inflammation》2014,37(5):1826-1836
The aim of this study was to investigate the potential anti-inflammatory and anti-oxidant effects of gabapentin (GBP) in mice. The anti-inflammatory and anti-oxidant effects were evaluated using various mediators that induce paw edema, peritonitis model, myeloperoxidase (MPO) activity, proinflammatory cytokine levels, glutathione (GSH) consumption, and malondialdehyde (MDA) production in mice. Pretreatment of mice with GBP (1 mg/kg) significantly reduced carrageenan or dextran-induced paw edema (P?0.05) when compared to vehicle group. Adding to this, GBP (1 mg/kg) significantly inhibited paw edema induced by histamine, serotonin, bradikinin, 48/80 compound, and prostaglandin E2. In the carrageenan-induced peritonitis model, GBP significantly decreased total and differential leukocyte counts and reduced the levels of MPO activity in the plantar tissue and IL-1β and TNF-α concentrations in the peritoneal exudate. The same dose of GBP also decreased the MDA concentration and increased the levels of GSH into the peritoneal fluid. In summary, our results demonstrated that GBP exhibited anti-inflammatory activity in mice by reducing the action of inflammatory mediators, neutrophil migration and proinflammatory cytokine levels, and anti-oxidant properties by decreasing the concentration of MDA and increasing the GSH content. These observations raise the possibility that GBP could be used to improve tissue resistance to damage during inflammatory conditions. 相似文献
946.
947.
948.
Ulisses C. Linhares Patrícia B. Schiavoni Priscila O. Barros Taissa M. Kasahara Bruna Teixeira Thais B. Ferreira Regina Alvarenga Joana Hygino Morgana M. M. Vieira Vera Carolina B. Bittencourt Regis M. Andrade Arnaldo F. Andrade Cleonice A. M. Bento 《Journal of clinical immunology》2013,33(1):179-189
Neuromyelitis optica (NMO), also known as Devic’s disease, is an autoimmune, inflammatory disorder of the central nervous system (CNS) in which the immune system attacks myelin of the neurons located at the optic nerves and spinal cord, thus producing a simultaneous or sequential optic neuritis and myelitis. The objective of this study was evaluated the background T-cell function of patients suffering from neuromyelitis optica (NMO), an autoimmune disorder of the central nervous system. In our study, the in vitro T cell proliferation and the production of Th1 cytokines were significantly lower in cell cultures from NMO patients, as compared with healthy individuals. In contrast, a dominant Th17-like phenotype, associate with higher IL-23 and IL-6 production by LPS-activated monocytes, was observed among NMO patients. The release of IL-21 and IL-6 by polyclonaly activated CD4+ T cells was directly correlated to neurological disability. In addition, the in vitro release of IL-21, IL-6 and IL-17 was significantly more resistant to glucocorticoid inhibition in NMO patients. In conclusion, the results indicate dominate Th17-related response in NMO patients that was directly proportional to neurological disability. Furthermore, our results can help to explain why NMO patients trend to be more refractory to corticoid treatment. 相似文献
949.
Gabriela Vera-Lozada Vanesa Scholl Mário Henrique M. Barros Davide Sisti Michele Guescini Vilberto Stocchi Claudio Gustavo Stefanoff Rocio Hassan 《Experimental and molecular pathology》2014
Formalin-fixed paraffin-embedded (FFPE) tissues are invaluable sources of biological material for research and diagnostic purposes. In this study, we aimed to identify biological and technical variability in RT-qPCR TaqMan® assays performed with FFPE-RNA from lymph nodes of classical Hodgkin lymphoma samples. An ANOVA-nested 6-level design was employed to evaluate BCL2, CASP3, IRF4, LYZ and STAT1 gene expression. The most variable genes were CASP3 (low expression) and LYZ (high expression). Total variability decreased after normalization for all genes, except by LYZ. Genes with moderate and low expression were identified and suffered more the effects of the technical manipulation than high-expression genes. Pre-amplification was shown to introduce significant technical variability, which was partially alleviated by lowering to a half the amount of input RNA. Ct and Cy0 quantification methods, based on cycle-threshold and the kinetic of amplification curves, respectively, were compared. Cy0 method resulted in higher quantification values, leading to the decrease of total variability in CASP3 and LYZ genes. The mean individual noise was 0.45 (0.31 to 0.61 SD), indicating a variation of gene expression over ~ 1.5 folds from one case to another. We showed that total variability in RT-qPCR from FFPE-RNA is not higher than that reported for fresh complex tissues, and identified gene-, and expression level-sources of biological and technical variability, which can allow better strategies for designing RT-qPCR assays from highly degraded and inhibited samples. 相似文献
950.
Cíntia Barros Santos-Rebou?as Stefanie Belet Luciana Guedes de Almeida Márcia Gon?alves Ribeiro Enrique Medina-Acosta Paulo Roberto Valle Bahia Ant?nio Francisco Alves da Silva Flávia Lima dos Santos Glenda Corrêa Borges de Lacerda Márcia Mattos Gon?alves Pimentel Guy Froyen 《European journal of human genetics : EJHG》2014,22(5):644-651