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排序方式: 共有226条查询结果,搜索用时 31 毫秒
151.
Rajeev Mehla Sandeep R.P. Kumar Pragya Yadav Pradip V. Barde Prasanna N. Yergolkar Bobbie R. Erickson Serena A. Carroll Akhilesh C. Mishra Stuart T. Nichol Devendra T. Mourya 《Emerging infectious diseases》2009,15(9):1431-1437
Kyasanur Forest disease virus (KFDV) is enzootic to India and maintained in ticks, mammals, and birds. It causes severe febrile illness in humans and was first recognized in 1957 associated with a high number of deaths among monkeys in Kyasanur Forest. Genetic analysis of 48 viruses isolated in India during 1957–2006 showed low diversity (1.2%). Bayesian coalescence analysis of these sequences and those of KFDVs from Saudi Arabia and the People’s Republic of China estimated that KFDVs have evolved at a mean rate of ≈6.4 × 10–4 substitutions/site/year, which is similar to rates estimated for mosquito-borne flaviviruses. KFDVs were estimated to have shared a common ancestor in ≈1942, fifteen years before identification of the disease in India. These data are consistent with the view that KFD represented a newly emerged disease when first recognized. Recent common ancestry of KFDVs from India and Saudi Arabia, despite their large geographic separation, indicates long-range movement of virus, possibly by birds. 相似文献
152.
Miranda PV; Gonzalez-Echeverria F; Marin-Briggiler CI; Brandelli A; Blaquier JA; Tezon JG 《Molecular human reproduction》1997,3(5):399-404
Glycosidic residues of the mammalian zona pellucida (ZP) are known to be
involved in sperm binding, suggesting the presence of complementary
carbohydrate binding sites on spermatozoa. However, in previous studies, in
which sperm suspensions were incubated with monosaccharides, no inhibitory
effect was observed. Results of studies in which sperm were treated shortly
after swim-up suggest that the use of non-capacitated cells may explain the
apparently conflicting results. In the present report, we studied the
effect of preincubation of capacitated spermatozoa with different
monosaccharides on their ability to bind to ZP. After 5 h under
capacitating conditions, spermatozoa were incubated in medium with or
without a monosaccharide, resuspended in fresh medium and used for hemizona
(HZ) binding assay. When ZH were incubated with spermatozoa treated with
N-acetyl-D- glucosamine, D-mannose, D-fucose, L-fucose or D-galactose, a
significant decrease in the number of spermatozoa bound was observed (level
of inhibition: 62, 58, 82, 68 and 48% respectively) while treatment of
spermatozoa with D-glucose produced no inhibition. Sugar treatment neither
altered sperm motility nor the rate of acrosome reaction. These results
suggest that N-acetylglucosamine, mannose, fucose and galactose residues
are involved in human sperm-zona pellucida binding in vitro.
相似文献
153.
Relationships of gp70 of MuLV envelopes to gp70 components of mouse lymphocyte plasma membranes 总被引:5,自引:0,他引:5 下载免费PDF全文
The family of glycoproteins called gp70 includes molecules that are the main constituent of murine C-type viral envelopes, and some that are expressed as mendelian constituents of thymocyte plasma membranes in the absence of virions. To investigate further the relation of viral gp70s to plasma- membrane gp70s we compared peptide maps of gp70s derived by immunoprecipitation from cells infected with chosen viruses and from various thymocytes and leukemiacells known to express one or more of three immunogenetically defined gp70 types: Glx-gp70, X-gp70, and O-gp70. Maps of gp70 from cultured cells infected with ecotropic and xenotropic viruses were distinguishable from one another, and in general resembled gp70 maps prepared directly from ecotropic and xenotropic virions respectively. Maps of gp70s immunoprecipitated from thymocytes of five mouse strains and from two A strain T-cell leukemias also fell into two distinguishable and generally corresponding patterns. Thus peptide-mapping substantiates earlier conclusions that viral gp70s and plasma-membrane gp70s inherited independently of virus-production are highly related or identical molecules. The gp70 maps of thymocytes from B6, B6-G(+IX), 129, and A mice formed a group resembling the map from cultured cells infected with xenotropic virus. Thymocytes from AKR mice, and the two A strain leukemias, gave gp70 maps conforming more to the second pattern, that of cultured cells infected with ecotropic virus. This second pattern probably comprises at least two gp70 types, one of which is X-gp70. Our data indicate that the G(IX)-gp70 and O-gp70 sub-species of gp70 expressed in the cell populations we have studied are coded by xenotropic viral genomes, and X-gp70 by ecotropic viral genomes. 相似文献
154.
Herpesvirus infections in solid organ transplant patients at high risk of primary cytomegalovirus disease 总被引:4,自引:0,他引:4
Razonable RR Brown RA Humar A Covington E Alecock E Paya CV;PV Study Group 《The Journal of infectious diseases》2005,192(8):1331-1339
The epidemiology of infections with 5 human herpesviruses (HHVs) (HHV-6, HHV-7, HHV-8, varicella zoster virus [VZV], and Epstein-Barr virus [EBV]) was investigated during the first year after solid organ transplantation in 263 patients who received oral ganciclovir or valganciclovir prophylaxis. HHV-6B DNAemia was uncommon, HHV-6A DNAemia was not observed, and HHV-7 DNAemia was prevalent. HHV-6 and HHV-7 DNAemia were not significantly associated with cytomegalovirus (CMV) disease, although a trend toward higher incidence of CMV disease was observed in HHV-6 DNAemic patients. VZV and HHV-8 DNAemia were not detected. EBV infection was common, although incidence of high-level EBV DNAemia was low, especially in patients who received valganciclovir prophylaxis. EBV-related posttransplant lymphoproliferative disease was not observed up to 12 months after transplantation. Compared with historic data, data from the present study suggest that antiviral prophylaxis may lower the incidence, prevalence, or level of DNAemia for infection with HHV-6, HHV-8, VZV, and EBV but not for infection with HHV-7. 相似文献
155.
Narasimh Gopalswamy MD Vishwanath N. Shenoy MD Umesh Choudhry MD Ronald J. Markert PhD Nancy Peace LPN Manoop S. Bhutani MD Christopher J. Barde MD 《Gastrointestinal endoscopy》1997,46(6):497-502
Background: Accurate measurement of polyp size during colonoscopy is important because of the direct correlation of size with colon cancer. Major studies of colorectal neoplasms have measured polyp size differently. It is also well documented that endoscopists underestimate polyp size frequently. The goal of this prospective study was to determine which one of the five methods of estimating polyp size during colonoscopy is most accurate. Methods: One hundred colon polyps were measured by means of visual estimation, open biopsy forceps methods, linear probe, a ruler immediately after excision, and after fixation in formalin. The size of the polyps measured outside the body immediately after excision was considered the “gold standard” against which all measurements were compared. Results: Forty-seven polyps were 5 mm or less in diameter, 33 polyps were 5.01 mm to 10 mm, and 20 polyps were more than 10 mm in size. For all polyps the mean difference versus the actual size of the polyps was 3.4% for linear probe, 6.4% for visual estimation, and 12.3% for the forceps. Conclusion: Measurement of polyp size by linear probe agreed best with the actual polyp size, followed closely by visual estimation. The open biopsy forceps method was the least accurate. (Gastrointest Endosc 1997;46:497-502.) 相似文献
156.
157.
Electrophysiological characterization of SCN5A mutations causing long QT (E1784K) and Brugada (R1512W and R1432G) syndromes 总被引:10,自引:0,他引:10
Deschênes I Baroudi G Berthet M Barde I Chalvidan T Denjoy I Guicheney P Chahine M 《Cardiovascular research》2000,46(1):55-65
Familial long QT syndrome (LQTS) and Brugada syndrome are two distinct human hereditary cardiac diseases known to cause ventricular tachyarrhythmias (torsade de pointes) and idiopathic ventricular fibrillation, respectively, which can both lead to sudden death. OBJECTIVE: In this study we have identified and electrophysiologically characterized, in patients having either LQTS or Brugada syndrome, three mutations in SCN5A (a cardiac sodium channel gene). METHOD: The mutant channels were expressed in a mammalian expression system and studied by means of the patch clamp technique. RESULTS: The R1512W mutation found in our first patient diagnosed with Brugada syndrome produced a slowing of both inactivation and recovery from inactivation. The R4132G mutation found in our second patient who also presented Brugada syndrome, resulted in no measurable sodium currents. Both Brugada syndrome patients showed ST segment elevation and right bundle-branch block, and had experienced syncopes. The E1784K mutation found in the LQTS showed a persistent inward sodium current, a hyperpolarized shift of the steady-sate inactivation and a faster recovery from inactivation. CONCLUSION: The different clinical manifestations of these three mutations most probably originate from the distinct electrophysiological abnormalities of the mutant cardiac sodium channels reported in this study. 相似文献
158.
The purpose of this article was to review the literature and provide guidelines on the use of radiation protection for patients in the dental setting. There are limited published data on the effects of low radiation doses such as those used in dental radiology. Most of the evidence is subject to bias, with risk models extrapolated from higher dose models such as studies of the Hiroshima bomb survivors. However, the lack of evidence does not denote the absence of risk, as there is no established ‘safe’ level of radiation exposure. All imaging utilizing ionizing radiation carries a risk for the patient. Hence the patient benefits of imaging must outweigh the potential risk. All diagnostic imaging should adhere to three basic principles, these being justification, optimization and application of dose limits. This article discusses dose reduction techniques and shielding of sensitive organs, particularly the thyroid, during procedures such as intraoral imaging, orthopantomograms and imaging of the pregnant patient. 相似文献
159.
Avinash Sharma Vinay Kulkarni Saurabh Barde Shrikant Bobade Varun Bhargava Ram Ghodeshwar 《Indian Journal of Thoracic and Cardiovascular Surgery》2009,25(1):29-30
Origin of isolated Left Distal Circumflex Coronary Artery (LCX) from pulmonary artery is a rare presentation especially in
adult age group. We report a case of a 68 year male suffering from coronary artery disease along with this anomaly. Surgical
correction comprising of off pump coronary artery bypass with ligation of anomalous distal LCX was performed successfully
in this case. 相似文献
160.