智能化穴位温度检测仪的研制及实验研究

在经络穴位的生物物理属性中，由于皮肤温度比较灵敏，易于观察，又能及时反映该处血管的舒缩变化。作者利用铂电阻作为测温控头，用微机进行数据处理，并利用该系统进行人体皮肤温度的检测及分析。结果表明：皮肤温差点基本上是循经分布的。     

工程化肌腱修复肌腱缺损后力学特性的组织学基础

探讨组织工程化肌腱修复肌腱缺损后体内愈合过程中力学特性的组织学基础。取罗曼鸡肌腱细胞 ,经体外培养、扩增 ,与可降解生物材料聚羟基乙酸筛网构建工程化肌腱 ;将其植入修复 2 0只罗曼鸡第二趾深屈肌腱0 .5～ 0 .8cm缺损。术后第 2、4、6、8周取材 ,对标本进行大体、组织学及生物力学测定。植入 2、4、6、8周 ,新生肌腱在大体形态、细胞及胶原纤维排列方式上与正常肌腱相似 ,但新生肌腱的胶原纤维束并未形成较多的沿肌腱长度方向的致密结构 (“塑形”) ,导致其最大张力增加缓慢 ,到 8周时为 15 .4 0± 10 .6 3N,仅达正常肌腱的 2 3 ;8周时最大张应变为 2 2 .4 9± 10 .2 1 ,比正常肌腱大 10。结果表明 ,单纯聚羟基乙酸作支架 ,材料降解过快 ,新生肌腱失去了正常的力学刺激 ,“塑形”能力差 ,其生物力学强度低。提示 ,保持新生肌腱形成过程中正常的力学刺激对新生肌腱的“塑形”可能是至关重要的。     

Requirement of the initial production of gamma interferon in the generation of protective immunity of mice against Listeria monocytogenes.

Protective immunity of mice against Listeria monocytogenes, which is mediated mainly by gamma interferon (IFN-gamma)-producing T cells, was induced by immunization with viable bacteria but not with killed bacteria. By comparing mice immunized with either viable or killed L. monocytogenes, it was found that IFN-gamma was produced at the initial stage only after immunization with viable bacteria. This finding prompted us to investigate the effect of neutralizing the IFN-gamma on the final generation of protective T cells against L. monocytogenes. When endogenous IFN-gamma was neutralized by administration of anti-IFN-gamma monoclonal antibody for the initial 2 days in mice immunized with viable bacteria, the generation of protective T cells on day 6 was completely blocked, as revealed by T-cell adoptive transfer. The generation of listeria-specific IFN-gamma-producing T cells was also abolished. These results clearly demonstrated that endogenous IFN-gamma, which is produced at the initial stage of immunization, actually plays a critical role in the generation of protective T cells against L. monocytogenes in vivo. Moreover, this study suggested that the lack of IFN-gamma-inducing ability is responsible for the inability of killed L. monocytogenes to induce protective T cells in mice.     

白细胞对血吸虫病肝微循环的影响

本实验在透射电镜下发现:慢性血吸虫病兔(n=10)肝细胞间有大量淋巴细胞,肝细胞膜及超微结构受损。肝窦壁及门管区小血管壁粘附有白细胞,包括中性粒细胞和淋巴细胞。窦内皮破坏,血管腔狭窄,窦内红细胞淤滞。巨噬细胞亦增生、肥大。白细胞内胞浆肿胀,富含溶酶体,微胞饮泡活跃。表明粘附、嵌塞和聚集在肝微循环内的白细胞可损伤肝实质及形成血吸虫病性门脉高压症。     

体外反搏对血液流变性和血小板聚集性的影响

观察４０例心脑血管疾病患者体外反搏前后血液流变性和血小板聚集性变化。结果表明，反搏后全血比及还原比粘度、血浆粘度有不同程度的下降（Ｐ＜０．０５～０．０１）。反搏治疗后，１～５ｍｉｎ血小板聚集率、最大聚集率和最大聚集速度较反搏前均明显降低（Ｐ＜０．０５～０．０１），而５ｍｉｎ解聚率则明显增加（Ｐ＜０．０５）。提示体外反搏治疗不仅影响血液动力学，而且明显降低血液粘度，对血小板聚集功能有明显抑制作用。     

Group-specific identification of polioviruses by PCR using primers containing mixed-base or deoxyinosine residue at positions of codon degeneracy.

We have developed a method for differentiating polioviruses from nonpolio enteroviruses using PCR. A pair of panpoliovirus PCR primers were designed to match intervals encoding amino acid sequences within VP1 that are strongly conserved among polioviruses. The initiating primer hybridizes with codons of a 7-amino-acid sequence that has been found only in polioviruses; the second primer matches codons of a domain thought to interact with the cell receptor. The panpoliovirus PCR primers contain mixed-base and deoxyinosine residues to compensate for the high degeneracy of the targeted codons. All RNAs from 48 vaccine-related and 110 wild poliovirus isolates of all three serotypes served as efficient templates for amplification of 79-bp product. None of the genomic sequences of 49 nonpolio enterovirus reference strains were amplified under equivalent reaction conditions. Sensitivities of poliovirus detection were as low as 100 fg (equivalent to approximately 25,000 genomic copies or 25 to 250 PFU) when the amplified products were visualized by ethidium bromide fluorescence. These degenerate PCR primers should aid in the detection of all polioviruses, including those wild poliovirus isolates for which genotype-specific reagents are unavailable.     

主动脉病变多层螺旋CT检查方法及其影像学特征

随着多层螺旋CT扫描速度和Z轴分辨率的提高，CT在显示血管的解剖与疾病方面得到很大发展，因此，CTA诊断血管疾病起着越来越重要的作用。本文着重介绍多层螺旋CT及CTA诊断主动脉病变的研究进展。     

经剑突下孔直接取石法在腹腔镜胆总管切开取石术中的应用

目的 探讨经剑突下孔直接取石法在腹腔镜胆总管切开取石术中的应用效果,并总结手术经验。方法 回顾性分析蚌埠市第一人民医院2014年11月-2018年4月86例胆总管结石患者的临床资料,其中男28例、女58例,年龄25~78(50.8±10.5)岁,均观察行腹腔镜手术,术中于胆总管切开后经剑突下孔直接取石。记录成功实施腹腔镜手术患者的手术时间、术中出血量、术后下床时间、术后排气时间、引流管拔除时间、术后住院时间、手术成功率及随访并发症,并总结技术经验。结果 成功完成腹腔镜下手术81例,成功率94.2%(81/86),其中1例因末端结石嵌顿难以取出,后经胆道镜下碎石取出。其余5例中,1例因胆囊十二指肠漏中转开放手术,4例因胆囊炎急性发作后三角区纤维化难以分离而中转开腹。81例成功手术者:手术时间为(80.2±18.6)min,术中出血量为 (20.0±3.0) mL,术后下床时间为(15.5±5.7)h,术后排气时间为(30.2±10.2)h,引流管拔除时间为术后(2.4±0.4)d,术后住院时间 (10.4±2.6) d。术后4~6周拔除T管;随访6~12个月,平均8.5个月,无术后胆漏、胆道狭窄、再发胆管结石等并发症。结论 经剑突下孔直接取石法使腹腔镜下胆总管切开取石术变得简单易行且安全,通过选择合适的患者,调整剑突下孔的位置、注意手术细节,改进手术流程,可减少了手术时间、创伤,并能取尽结石,获得满意的疗效。     

Controlled elimination of intracellular H(2)O(2): regulation of peroxiredoxin, catalase, and glutathione peroxidase via post-translational modification

The predominant enzymes responsible for elimination of hydrogen peroxide (H(2)O(2)) in cells are peroxiredoxins (Prxs), catalase, and glutathione peroxidases (GPxs). Evidence suggests that catalytic activities of certain isoforms of these H(2)O(2)-eliminating enzymes are extensively regulated via posttranslational modification. Prx I and Prx II become inactivated when phosphorylated on Thr(90) by cyclin B-dependent kinase Cdc2. In addition, the active-site cysteine of Prx I-IV undergoes a reversible sulfinylation (oxidation to cysteine sulfinic acid) in cells. Desulfinylation (reduction to cysteine) is achieved by a novel enzyme named sulfiredoxin. c-Abl and Arg nonreceptor protein tyrosine kinases associate with catalase in cells treated with H(2)O(2) by mechanisms involving the SH3 domains of the kinases and the Pro(293)PheAsnPro motif of catalase and activate catalase by phosphorylating it on Tyr(231) and Tyr(386). Similarily, GPx1 is activated by c-Abl- and Arg-mediated phosphorylation. The tyrosine phosphorylation is critical for ubiquitination-dependent degradation of catalase.