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951.
Modulatingefectofmitomycinorcisplatinonlymphokineactivatedkilercelproliferationandantitumoractivitytobladdercancercellinesin... 相似文献
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Shisan Bao Susan J. McClure David L. Emery Alan J. Husband 《European journal of immunology》1996,26(3):552-556
Interleukin (IL)-5 is produced by a variety of cell types and contributes to both lymphocyte development and eosinophil terminal differentiation in vitro. The coincidence of worm expulsion and eosinophilia in sheep infected with the gastrointestinal nematode Trichostrongylus colubriformis suggests that eosinophils may be involved as effector cells in host immunity against parasite infection. The role of IL-5 in this process was investigated by observing the distribution of IL-5 mRNA+ cells in the small intestine, mesenteric lymph nodes (MLN) and Peyer's patches (PP) by an in situ hybridization technique using a murine IL-5 riboprobe. IL-5 mRNA+ cells were distributed throughout the lamina propria (LP) of the small intestine from the tips of the villi to the muscularis mucosae and in the parafollicular areas of MLN and PP in both naive and immune sheep. The phenotypes of IL-5 mRNA+ cells was explored by simultaneous eosin and immunohistochemical staining using a monoclonal antibody recognizing the T19 marker, which identifies a major subset of γ/α TCR+ cells in sheep. In immune sheep, there was about a five-fold increase in the number of eosinophils and IL-5 mRNA+ cells in the LP, but there was no significant change in numbers of T19+ cells. Most of the IL-5 mRNA+ cells in the LP were eosinophils, but many of the T19+ cells also expressed IL-5 mRNA. In contrast, there were fewer eosinophils than T19+ cells in MLN of immune sheep and, compared to controls, a threefold increase in T19+ cells and a five-fold increase in T19+/IL-5 mRNA+ double-positive cells was observed in immune sheep. In PP, there were very few eosinophils but substantial numbers of T19+ cells; however, no significant differences in numbers of eosinophils, T19+ or IL-5 mRNA+ cells were observed between control and immune sheep. These results indicate that in sheep, both eosinophils and γ/δ T cells are capable of IL-5 expression and suggest that IL-5 is an important regulatory factor in autocrine and paracrine activation of effector cells involved in parasite immune expulsion. 相似文献
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954.
Imaging of sinonasal inflammatory disease 总被引:10,自引:0,他引:10
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OBJECTIVE: With the pComb3X-displaying Fab antibody libraries, to achieve the humanization of murine HAb18 against HCC by guided selection. METHODS: With the optimized primers, the human Fd and C(L) repertoire genes were amplified by RT-PCR from PBMC of HCC patients. The Fd repertoire genes were paired with murine HAb18 C(L) gene to construct pComb3X-displaying hybrid Fab library. The recombinant HAb18GE was used as antigens to select the target antibodies and got the Fd fragments. Then the human C(L) genes were paired with the selected human Fds to construct human Fab library. After the panning, the complete human Fab antibodies were got and analyzed. RESULTS: With the murine HAb18 C(L) gene as template, the heavy chain Fd shuffling was achieved by panning the hybrid Fab library. Then with the selected Fds as template, the human Fabs were obtained through the light chain shuffling. Two of the resulting human Fabs (HuFab2 and HuFab11), with same Fd and different light chains, bound to HAb18G/CD147 specifically. The competitive ELISA, Western blotting, FCM, fluorescent cell staining and so on demonstrated that the human Fabs resembled its parental murine Fab in that they both perhaps recognized the same epitope. K(D) indicated (HuFab2=210 nm and HuFab11=280 nm) the selected Fabs had available affinity. CONCLUSION: Through guided-selection, we got the available human Fab antibodies for the subsequent research. These results suggest that guided selection is a promising strategy in murine mAb humanization. 相似文献
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Lazar HL Bao Y Tanzillo L O'Gara P Reardon D Price D Crowley R Cabral HJ 《Journal of cardiac surgery》2005,20(6):519-523
BACKGROUND AND AIM: This study sought to determine whether the favorable anti-inflammatory effects of aprotinin might limit ischemic damage during the revascularization of ischemic myocardium. METHODS: Twenty pigs underwent 90 minutes of coronary occlusion followed by 45 minutes of blood cardioplegic arrest and 180 minutes of reperfusion. Ten animals received a loading dose of aprotinin (40,000 kallikrein inhibiting units/kg) during the start of coronary occlusion followed by an infusion of 20,000 kallikrein inhibiting units/kg/hour. Ten other animals received no aprotinin. Summary statistics are expressed as the mean +/- standard error. RESULTS: The aprotinin-treated animals required less cardioversions for ventricular arrhythmias (1.0 +/- 0.7 vs. 3.6 +/- 0.6; p < 0.001), accumulated less lung water (1.0 +/- 0.2% change vs. 6.2 +/- 0.9% change; p = 0.038), had more complete coronary relaxation to bradykinin (34.1 +/- 5.9% change vs. 9.2 +/- 3.5% change; p = 0.01), and had reduced infarct size (area necrosis/area risk = 20 +/- 1.1% vs. 39 +/- 1.2%; p = 0.003). CONCLUSIONS: Aprotinin limits ischemic injury during acute coronary revascularization by decreasing ventricular arrhythmias and lung edema, preserving endothelial function, and minimizing myocardial necrosis. 相似文献
960.
To compare the effect of standard trauma craniectomy (STC) versus limited craniectomy (LC) on the outcome of severe traumatic brain injury (TBI) with refractory intracranial hypertension, we conducted a study at five medical centers of 486 patients with severe TBI (Glasgow Coma Scale score = 8) and refractory intracranial hypertension. In all 486 cases, refractory intracranial hypertension, caused by unilateral massive frontotemporoparietal contusion, intracerebral/subdural hematoma, and brain edema, was confirmed on a CT scan. The patients were randomly divided into two groups, one of which underwent STC (n = 241) with a unilateral frontotemporoparietal bone flap (12 x 15 cm), and the second of which underwent LC (n = 245) with a routine temporoparietal bone flap (6 x 8 cm). At 6-month follow-up, 96 patients (39.8%) in the STC group had a favorable outcome on the basis of the Glasgow Outcome Scale, including 62 patients who had a good recovery and 34 who showed moderate deficits. Another 145 patients (60.2%) in the STC group had an unfavorable outcome, including 73 with severe deficits, nine with persistent vegetative status, and 63 who died. By comparison, only 70 patients (28.6%) in the LC group had a favorable outcome, including 41 who had a good recovery and 29 who had moderate deficits. Another 175 patients (71.4%) in the LC group had an unfavorable outcome, including 82 with severe deficits, seven with persistent vegetative status, and 86 who died (p < 0.05). In addition to these findings, the incidence of delayed intracranial hematoma, incisional hernia, and CSF fistula was lower in the STC group than in the LC group (p < 0.05), although the incidence of acute encephalomyelocele, traumatic seizure, and intracranial infection was not significantly different in the two groups (p > 0.05). The results of the study indicate that STC significantly improves outcome in severe TBI with refractory intracranial hypertension resulting from unilateral frontotemporoparietal contusion with or without intracerebral or subdural hematoma. This suggests that STC, rather than LC, be recommended for such patients. 相似文献