Protection from oxidant injury by sodium-dependent GSH uptake in retinal Müller cells.

Glutathione (GSH) is known to play an important role in regulating oxidative damage to cells. The present study was initiated to examine the effect of exogenous GSH on oxidative injury in a retinal Müller cell line and to characterize GSH transport in these cells. Rat Müller cells (rMC-1) were incubated with varying concentrations of t-butylhydroperoxide (t-BHP) to induce oxidative stress, and cell viability was measured after addition of GSH. In other studies, kinetics of GSH uptake and Na+-dependency were examined by incubating cells with35S-GSH in Na+-containing and Na+-free buffers. GSH uptake was studied with GSH at concentrations varying from 0. 05-10 m m in NaCl buffer. In the presence of sodium, extracellular GSH provided protection against t-BHP-induced oxidant injury to rMC-1 cells; in contrast, the amino acid precursors of GSH did not have any effect on cell viability. GSH was taken up by rMC-1 cells in a concentration- and sodium-dependent manner. Kinetic studies revealed both a high affinity (Km approximately 0.31 m m) and low affinity Km( approximately 4.2 m m) component. Furthermore, GSH depletion had no significant effect on the rate of GSH uptake. The results show that physiological concentrations of GSH can protect Müller cells from oxidative injury. Both Na+-dependent and Na+-independent transport systems for GSH exist in Müller cells, and the Na+-dependent GSH transporter may be involved in the protective role of GSH.     

高频透热撕囊法在白内障手术中的应用

目的:评价在白内障手术中使用高频透热撕囊仪作连续环形撕囊的效果。方法:在30例(34眼)患者进行白内障囊外摘出联合后房型人工晶体植入术中,先向前房注入透明质酸钠,然后使用高频透热撕囊仪作晶体连续环形撕囊术。结果:有3眼术中出现小的晶体前囊放射状撕裂,所有病例术后角膜无水肿,无其他术后合并症,术后一周31眼矫正视力≥0.5,占91.2％。结论:此法操作简便,易于掌握,是一个安全有效的连续环形撕囊的方法。尤其适用于过熟期白内障,儿童先天性白内障和外伤性白内障的截囊。眼科学报1999;15:121—123。     

后巩膜加固术对视网膜振荡电位的影响

目的　为了更进一步地了解后巩膜加固术治疗高度进行性近视的疗效和机理。方法　采用日本产Ｎｅｕｒｏｐａｃｋ Ｆｏｕｒ ４１０４ Ｋ 电生理仪,对２６ 例５２ 眼高度进行性近视行后巩膜加固术前、术后视网膜振荡电位进行定量分析。结果　术前、术后振荡电位总振幅经统计学处理有非常显著性差异,波的潜伏期无显著性差异。结论　本文认为后巩膜加固术可改善视网膜血液循环,并对机理进行了探讨     

国产生长激素治疗生长激素缺乏症的疗效观察

了解国产人生长激素（ｈＧＨ）的治疗效果。方法：对１５例未经生长激素治疗的生长激素缺乏症（ＧＨＤ）病人（均经精氨酸和可乐宁两项生长激素激发试验明确诊断,生长激素峰值均小于 １０ μｇ／Ｌ）应用国产分泌型基因重组人生长激素（ｒｈＧＨ）进行治疗,剂量为０．１ｕ／（ｋｇ·ｄ）睡前脐周皮下注射,治疗时间６个月。结果：１５例ＧＨＤ病人６个月身高平均增长（６．３４±０．９４）ｃｍ,生长速度由治疗前的（３．３３±０．４９）ｃｍ／年增加到治疗后的（１２．６８±１．８６）ｃｍ／年。结论：国产人生长激素治疗效果达到国外同类产品水平,而价格仅为进口产品的１／６左右。可安全有效地用于治疗ＧＨＤ身材矮小病人。     

踝部骨折有关问题的探讨

本文报导125例髁部骨折，其中52例严重骨折随访8个月至5年2个月。根据本组资料，作者对下胫腓关节损伤的诊断和处理、闭合治疗中骨折再移位问题以及50岁以上患者踝部骨折的治疗特点作了讨论。     

Ductal exfoliated cytology in the practice of mumps]

The author used smear from Stensen's duct aspiration to observe exfoliated cytology in order to help diagnosis of early mumps.According to the smears from 100 cases of mumps and other 50 cases of bacteria parotitis and/or normal parotid as contro,the author revealed that if there are a lot of ductal epithelial cells,monocytes and polynuclear cells,monocytes and polynuclear phagocytes at same time,it could give diagnosis "Mumps" to that patient.In control group,it is unable to observe those three kind cells at same time.The author also discussed the mechanism of how would these three kind cells appear in mumps in accordance with references.     

辨证施治冠心病48例临床疗效分析

本文对1974年11月～1976年12月,经中西医结合治疗的48例冠心病,作一回顾性分析,着重探索辨证施治在冠心病的中西医结合治疗中的优越性及如何提高临床疗效等问题。材料与方法一、一般资料:48例中,男42例,女6例:年龄38～85岁,除1例外均在40岁以上。二、病例选择及分型:全部病例均按1974年冠心病、高血压普查预防座谈会修订标准进行诊断;47例属确诊冠心病,1例属可疑冠心病。其中:(1)以心     

褐苞薯蓣对小鼠脾虚证的影响

目的：观察褐苞薯蓣对小鼠脾虚证的影响。方法：用利血平致小鼠脾虚模型，以其体重、体温、胸腺及脾重、血清刚果红与木糖含量为指标，观察实验结果。结果：褐苞薯蓣能使体重、体温、胸腺重、脾重、血清木糖含量增加，对血清刚果红含量减少不显著。怀山药的结果与褐苞薯蓣相似，但对后3个指标影响不明显。结论：褐苞薯蓣有补脾作用和特异性免疫作用，怀山药与其相似，但略逊。     

激光治疗闭角型青光眼

目的：评价激光虹膜切除治疗闭角型青光眼临床疗效。方法：应用Nd：YAG激光于上方或下方作周边虹膜切除。结果：107例122眼闭角型青光眼术后经过2—106月随访观察，成功115眼，失败7眼。结论：激光虹膜切除术治疗闭角型青光眼安全、有效。     

乌梢蛇与其混淆品白条锦蛇的鉴别

 目的比较鉴别乌梢蛇与白条锦蛇。方法对乌梢蛇与其混淆品白条锦蛇的生药性状、薄层色谱、纸色谱、紫外吸收光谱进行了鉴别研究。结果乌梢蛇与白条锦蛇的生药性状、薄层色谱有明显差异，纸色谱、紫外吸收光谱差异不大。结论白条锦蛇不能混充乌梢蛇入药，应予以鉴别。