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排序方式: 共有10000条查询结果,搜索用时 15 毫秒
991.
992.
R Mandeville F Dumas A Amarouch S Sidrac-Ghali M C Walker M Zelechowska I Ajdukovic B Grouix 《Hybridoma》1987,6(5):441-451
This study reports the purification and characterization of a high molecular weight human breast cancer-associated antigen identified by a previously described (1,2) murine monoclonal antibody, BCD-B4. Immunohistochemical analysis indicated that BCD-B4 recognizes an antigen expressed in an altered form on the human breast carcinoma cell line, BT-20, compared to the non-malignant human mammary epithelial cell line, HBL-100. Chemical treatments and enzymatic digestions suggested that the recognized moiety was a protein. The antigenic determinant was resistant to neuraminidase and periodate treatments but was sensitive to trypsin and proteinase K. The antigen was purified by affinity chromatography and its molecular weight, determined by SDS-PAGE analysis under non-reducing conditions, was proven to be 250 Kd. Under reducing conditions, the molecule dissociated into two polypeptides of 125 and 45 Kd, respectively. Both subunits could be isolated from normal HBL-100 and neoplastic BT-20 cellular protein extracts by affinity chromatography. The higher molecular weight subunit showed; however, qualitative and quantitative differences between the two cell lines: it was expressed in greater quantity on BT-20 cells and its molecular weight was 15 Kd higher. Both subunits could also be identified by immunoblots of BT-20 cells. 相似文献
993.
994.
995.
996.
Janette B Gomos Theresa M Rowe Saumendra N Sarkar Sean P Kessler Ganes C Sen 《Journal of interferon & cytokine research》2002,22(2):199-206
The 9-2 isozyme of 2-5 (A) synthetase has cellular proapoptotic functions that are mediated not by enzyme activity but by the Bcl-2 homology domain 3 present in its unique carboxyl-terminal region. Another proapoptotic cellular protein is Bax, whose absence in the Bax(-/-) mice causes male sterility due to abnormal sperm differentiation. In this study, we examined whether transgenic 9-2 expression can substitute for the in vivo reproductive function of Bax. To achieve this goal, a sperm-specific promoter was used to drive the expression of 9-2 in the sperm of transgenic mice. By selective cross-breeding, the transgene was transferred to Bax(-/-) mice to generate the experimental mouse line (Bax(-/-), 9-2(+/+)). The male experimental mice were sterile, and their testes maintained the structural abnormality found in Bax(-/-) mice. Thus, the male reproduction functions of Bax could not be replaced by the 9-2 isozyme of 2-5 (A) synthetase. 相似文献
997.
998.
With the increased use of indwelling central venous catheters, increasing numbers of cases of Rhodotorula fungemia have been observed in patients with neoplasia and neutropenia. In most patients with catheter-related Rhodotorula fungemia, the condition has been treated with broadspectrum antibiotics. We report two cases of central venous catheter-related
Rhodotorula rubra fungemia that occurred in patients with acute myeloblastic leukemia. Both patients were in a state of neutropenia. One patient
was treated with amphotericin B and his central venous catheter was removed, but he died of Klebsiella pneumoniae bacteremia. The other patient was treated with amphotericin B and discharged, with a central venous catheter, after recovery
from neutropenia. Although the management of catheter-related Rhodotorula fungemia infections remains controversial, resolution of the underlying disease is more important than catheter removal for
recovery from Rhodotorula rubra fungemia.
Received: August 22, 2001 / Accepted: October 8, 2001 相似文献
999.
Hinrich Böhner Detlef Kindgen-Milles Andreas Grust Rolf Buhl Wolf-Christian Lillotte Barbara T. Müller Eckhard Müller Günter Fürst Wilhelm Sandmann 《Langenbeck's archives of surgery / Deutsche Gesellschaft fur Chirurgie》2002,387(1):21-26
BACKGROUND: The efficacy of nasal continuous positive airway pressure (nCPAP) as a prophylactic method for preventing cardiopulmonary complications after major vascular surgery has not been investigated. PATIENTS/METHODS: In a prospective randomized trial, 204 patients undergoing elective midline laparotomy for vascular surgery were randomized to receive standard therapy ( n=105) or additional prophylactic nCPAP ( n=99) for the first postoperative night. Postoperative oxygenation, incidence of severe cardiac, and pulmonary complications, length of intensive care surveillance and length of total postoperative hospital stay (LOS) were compared. RESULTS: Prophylactic nCPAP significantly reduced the number of patients with severe oxygenation disturbances defined as paO(2) < 70 mmHg with FiO(2) > or = 0.7 (5 versus 17, P=.01). There were no differences with respect to death, cardiac and pulmonary complications, length of intensive care surveillance or LOS. CONCLUSION: Prophylactic 12 h nCPAP significantly reduces the occurrence of postoperative oxygenation disturbances but has no effect on cardiac or pulmonary complications, need for intensive care, LOS or mortality after major vascular surgery. 相似文献
1000.
F. Berndsen D. Arvidsson L.-K. Enander C.-E. Leijonmarck U. Wingren C. Rudberg S. Smedberg G. Wickbom A. Montgomery 《Hernia》2002,6(2):56-61
Interest in inguinal hernia surgery has increased significantly with the introduction of new operating techniques during
the past decade. This multicenter study compared short-term results in patients treated by the laparoscopic transabdominal
preperitoneal patch technique (TAPP; n=518) and the Shouldice technique (n=524). We evaluated demographics, operative data, complications, hospital stay, postoperative pain, use of analgesics, functional
status, sick leave, and complaints up to 3 months postoperatively. The median operating time was shorter in the Shouldice
group (55 vs. 65 min), but there were no significant differences in complication rates, and major complications were rare.
The hospital stay was 1 day or less in over 98% of cases in both groups, but more operations were performed on outpatient
basis in the Shouldice group. In the TAPP group postoperative pain and analgesic consumption were less, postoperative functional
status was better, and sick leave was shorter (10 vs. 14 days). These results show that the two methods are equally safe and
have few major complications. The TAPP operation is associated with less postoperative pain, better postoperative functional
status, and shorter sick leave, but at the price of a longer operating time.
Electronic Publication 相似文献