全文获取类型
收费全文 | 7851篇 |
免费 | 523篇 |
国内免费 | 51篇 |
专业分类
耳鼻咽喉 | 32篇 |
儿科学 | 124篇 |
妇产科学 | 98篇 |
基础医学 | 1140篇 |
口腔科学 | 160篇 |
临床医学 | 709篇 |
内科学 | 2236篇 |
皮肤病学 | 257篇 |
神经病学 | 383篇 |
特种医学 | 270篇 |
外科学 | 1271篇 |
综合类 | 31篇 |
一般理论 | 1篇 |
预防医学 | 227篇 |
眼科学 | 86篇 |
药学 | 473篇 |
中国医学 | 21篇 |
肿瘤学 | 906篇 |
出版年
2023年 | 52篇 |
2022年 | 93篇 |
2021年 | 193篇 |
2020年 | 75篇 |
2019年 | 134篇 |
2018年 | 184篇 |
2017年 | 116篇 |
2016年 | 165篇 |
2015年 | 146篇 |
2014年 | 215篇 |
2013年 | 260篇 |
2012年 | 416篇 |
2011年 | 431篇 |
2010年 | 232篇 |
2009年 | 213篇 |
2008年 | 381篇 |
2007年 | 366篇 |
2006年 | 380篇 |
2005年 | 377篇 |
2004年 | 367篇 |
2003年 | 373篇 |
2002年 | 285篇 |
2001年 | 269篇 |
2000年 | 304篇 |
1999年 | 279篇 |
1998年 | 101篇 |
1997年 | 76篇 |
1996年 | 73篇 |
1995年 | 57篇 |
1994年 | 55篇 |
1993年 | 72篇 |
1992年 | 185篇 |
1991年 | 189篇 |
1990年 | 183篇 |
1989年 | 165篇 |
1988年 | 142篇 |
1987年 | 109篇 |
1986年 | 90篇 |
1985年 | 95篇 |
1984年 | 70篇 |
1983年 | 49篇 |
1982年 | 24篇 |
1981年 | 29篇 |
1980年 | 23篇 |
1979年 | 44篇 |
1978年 | 22篇 |
1977年 | 28篇 |
1976年 | 25篇 |
1970年 | 22篇 |
1968年 | 22篇 |
排序方式: 共有8425条查询结果,搜索用时 31 毫秒
61.
Argyrophil cells and melanocytes in esophageal mucosa 总被引:5,自引:0,他引:5
62.
63.
Koichiro Yuji Shigesaburo Miyakoshi Daisuke Kato Yuji Miura Tomohiro Myojo Naoko Murashige Yukiko Kishi Kazuhiro Kobayashi Eiji Kusumi Hiroto Narimatsu Tamae Hamaki Tomoko Matsumura Masahiro Kami Takahiro Fukuda Shigeru Masuo Kazuhiro Masuoka Atsushi Wake Junichi Ueyama Akiko Yoneyama Ko Miyamoto Haruhisa Nagoshi Michio Matsuzaki Shinichi Morinaga Yoshitomo Muto Yoichi Takeue Shuichi Taniguchi 《Biology of blood and marrow transplantation》2005,11(4):314-318
We report the results of reduced-intensity unrelated cord blood transplantation (RI-UCBT) in patients with advanced malignant lymphoma. Twenty patients (median age, 46.5 years; range, 27-66 years) underwent RI-UCBT with a preparative regimen consisting of fludarabine 125 mg/m2 , melphalan 80 mg/m 2 , and 4 Gy of total body irradiation. The median infused total cell dose was 2.75 x 10(7)/kg (range, 2.3-3.4 x 10(7)/kg). Graft-versus-host disease (GVHD) prophylaxis was composed of cyclosporine or tacrolimus alone. Fifteen patients achieved primary neutrophil engraftment after a median of 20 days. Eight patients developed grade II to IV acute GVHD, and 2 developed chronic GVHD. Of the 16 patients with evaluable disease, 10 achieved a complete response. Primary disease recurred in 1 patient, and transplant-related mortality within 100 days occurred in 8 of 20 patients. The estimated 1-year probability of progression-free survival was 50%. These data suggest that RI-UCBT is a feasible option for patients with refractory lymphoma who lack an HLA-matched donor. 相似文献
64.
Enhanced expression of type I interferon and toll-like receptor-3 in primary biliary cirrhosis 总被引:6,自引:0,他引:6
Takii Y Nakamura M Ito M Yokoyama T Komori A Shimizu-Yoshida Y Nakao R Kusumoto K Nagaoka S Yano K Abiru S Ueki T Matsumoto T Daikoku M Taniguchi K Fujioka H Migita K Yatsuhashi H Nakashima M Harada M Ishibashi H 《Laboratory investigation; a journal of technical methods and pathology》2005,85(7):908-920
65.
Tanaka G Kanaji S Hirano A Arima K Shinagawa A Goda C Yasunaga S Ikizawa K Yanagihara Y Kubo M Kuriyama-Fujii Y Sugita Y Inokuchi A Izuhara K 《International immunology》2005,17(6):797-805
It is widely known that IL-4 and IL-13 act on various kinds of cells, including B cells, resulting in enhancement of proliferation, class switching to IgE and expression of several surface proteins. These functions are important for the recognition of the various antigens in B cells and are known to be involved in the pathogenesis of allergic diseases. However, it has not been known whether IL-4/IL-13 is involved in the metabolism of various kinds of xenobiotics including 2,3,7,8-tetra-chlorodibenzo-p-dioxin (TCDD), and it remains undetermined whether TCDD, an environmental pollutant, influences IgE production in B cells, exaggerating allergic reactions. We identified IL-4- or IL-13-inducible genes in a human Burkitt lymphoma cell line, DND-39, using microarray technology, in which the AHR gene was included. The AHR gene product, the aryl hydrocarbon receptor (AhR), was induced by IL-4 in both mouse and human B cells in a STAT6-dependent manner. IL-4 alone had the ability to translocate the induced AhR to the nuclei. TCDD, a ligand for AhR, rapidly degraded the induced AhR by the proteasomal pathway, although IL-4-activated AhR sustained its expression. AhR activated by IL-4 caused expression of a xenobiotic-metabolizing gene, CYP1A1, and TCDD synergistically acted on the induction of this gene by IL-4. However, the induction of AhR had no effect on IgE synthesis or CD23 expression. These results indicate that the metabolism of xenobiotics would be a novel biological function of IL-4 and IL-13 in B cells, whereas TCDD is not involved in IgE synthesis in B cells. 相似文献
66.
Loss of mammalian Sprouty2 leads to enteric neuronal hyperplasia and esophageal achalasia 总被引:3,自引:0,他引:3
Taketomi T Yoshiga D Taniguchi K Kobayashi T Nonami A Kato R Sasaki M Sasaki A Ishibashi H Moriyama M Nakamura K Nishimura J Yoshimura A 《Nature neuroscience》2005,8(7):855-857
We report here that loss of the Sprouty2 gene (also known as Spry2) in mice resulted in enteric nerve hyperplasia, which led to esophageal achalasia and intestinal pseudo-obstruction. Glial cell line-derived neurotrophic factor (GDNF) induced hyperactivation of ERK and Akt in enteric nerve cells. Anti-GDNF antibody administration corrected nerve hyperplasia in Sprouty2-deficient mice. We show Sprouty2 to be a negative regulator of GDNF for the neonatal development or survival of enteric nerve cells. 相似文献
67.
A combination of interleukin-6 and its soluble receptor impairs sperm motility: implications in infertility associated with endometriosis 总被引:7,自引:0,他引:7
Yoshida S Harada T Iwabe T Taniguchi F Mitsunari M Yamauchi N Deura I Horie S Terakawa N 《Human reproduction (Oxford, England)》2004,19(8):1821-1825
BACKGROUND: We previously reported that the level of interleukin (IL)-6 is increased in the peritoneal fluid of women with endometriosis. This study was undertaken to assess the effects of IL-6 and soluble IL-6 receptor (sIL-6R) on in vitro sperm motility. METHODS: Sperm (n = 20) were cultured with IL-6 or sIL-6R, or with a combination of both. After 24 h cultures, sperm motility was evaluated using a computer-assisted semen analysis system. Gene and protein expressions of IL-6, IL-6 receptor (IL-6R), and glycoprotein 130 (gp130) were examined in sperm by RT-PCR analysis and western blot analysis. RESULTS: Addition of IL-6 or sIL-6R individually to the culture media had no affect on sperm motion. However, adding a combination of IL-6 and sIL-6R dose-dependently reduced the percentage of motile and rapidly moving sperm. Adding anti-IL-6R antibody abolished these adverse effects. Sperm expressed the gp130 gene and protein, but not IL-6 or IL-6R. CONCLUSIONS: A combination of IL-6 and sIL-6R may be associated with gp130 expressed in the sperm and reduce sperm motility. IL-6 and sIL-6R may contribute to the pathogenesis of endometriosis-associated infertility. 相似文献
68.
Kanamycin ototoxicity in glutamate transporter knockout mice 总被引:2,自引:0,他引:2
Glutamate-aspartate transporter (GLAST), a powerful glutamate uptake system, removes released glutamate from the synaptic cleft and facilitates the re-use of glutamate as a neurotransmitter recycling system. Aminoglycoside-induced hearing loss is mediated via a glutamate excitotoxic process. We investigated the effect of aminoglycoside ototoxicity in GLAST knockout mice using the recorded auditory brainstem response (ABR) and number of hair cells in the cochlea. Kanamycin (100 mg/mL) was injected directly into the posterior semicircular canal of mice. Before the kanamycin treatment, there was no difference in the ABR threshold average between the wild-type and knockout mice. Kanamycin injection aggravated the ABR threshold in the GLAST knockout mice compared with the wild-type mice, and the IHC degeneration was more severe in the GLAST knockout mice. These findings suggest that GLAST plays an important role in preventing the degeneration of inner hair cells in aminoglycoside ototoxicity. 相似文献
69.
Tamiya G Shinya M Imanishi T Ikuta T Makino S Okamoto K Furugaki K Matsumoto T Mano S Ando S Nozaki Y Yukawa W Nakashige R Yamaguchi D Ishibashi H Yonekura M Nakami Y Takayama S Endo T Saruwatari T Yagura M Yoshikawa Y Fujimoto K Oka A Chiku S Linsen SE Giphart MJ Kulski JK Fukazawa T Hashimoto H Kimura M Hoshina Y Suzuki Y Hotta T Mochida J Minezaki T Komai K Shiozawa S Taniguchi A Yamanaka H Kamatani N Gojobori T Bahram S Inoko H 《Human molecular genetics》2005,14(16):2305-2321
A major goal of current human genome-wide studies is to identify the genetic basis of complex disorders. However, the availability of an unbiased, reliable, cost efficient and comprehensive methodology to analyze the entire genome for complex disease association is still largely lacking or problematic. Therefore, we have developed a practical and efficient strategy for whole genome association studies of complex diseases by charting the human genome at 100 kb intervals using a collection of 27,039 microsatellites and the DNA pooling method in three successive genomic screens of independent case-control populations. The final step in our methodology consists of fine mapping of the candidate susceptible DNA regions by single nucleotide polymorphisms (SNPs) analysis. This approach was validated upon application to rheumatoid arthritis, a destructive joint disease affecting up to 1% of the population. A total of 47 candidate regions were identified. The top seven loci, withstanding the most stringent statistical tests, were dissected down to individual genes and/or SNPs on four chromosomes, including the previously known 6p21.3-encoded Major Histocompatibility Complex gene, HLA-DRB1. Hence, microsatellite-based genome-wide association analysis complemented by end stage SNP typing provides a new tool for genetic dissection of multifactorial pathologies including common diseases. 相似文献
70.
Sato A Taniguchi I Fujiwara D Ichikawa H Suzuki M Nawata S Murakami G 《Anatomical science international / Japanese Association of Anatomists》2003,78(4):211-222
Gaps and fragmentation of the superficial lymph node cortex are considered to provide intranodal shunt flow between the afferent
and efferent vessels. Using serial sections of 205 nodes obtained from 27 donated cadavers more than 70 years of age, we examined
the histological architecture of the abdominal and pelvic nodes in elderly Japanese. Secondary follicles were rare in the
specimens. Cortex gaps were, to a greater or lesser degree, found in all nodes. We classified these nodes into three types
according to how often the gap occurred. Type 1 nodes, with a relatively complete shield for the afferent lymph, were most
frequently found in gastric nodes, whereas type 3 nodes, with numerous gaps, were often observed in the colic, para-aortic
and pelvic nodes. The type 3 nodes showed a specific architecture characterized by a fragmented superficial cortex, three-dimensionally
assembled cords and a common sinus between them. Primary follicles were located in the assembled cord structures as well as
at the superficial cortex. Irrespective of the type, B and T lymphocyte areas were intermingled in the cortex-like areas.
The present results reveal region-specific histological heterogeneity in aged human visceral nodes. Due to increased surface
areas, the type 3 architecture seemed to accelerate systemic immunity rather than act as a local barrier in the para-aortic
and pelvic nodes, which are located centrally along the lymphatic drainage routes. However, thick trabeculae often seemed
to develop in the type 3 sinus to decrease nodal function with aging. 相似文献