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991.
Variations in target volume definition and dose to normal tissue using anatomic versus biological imaging (18F‐FDG‐PET) in the treatment of bone metastases: results from a 3‐arm randomized phase II trial 下载免费PDF全文
992.
AM Slavotinek A Moshrefi N Lopez Jiminez R Chao A Mendell GM Shaw LA Pennacchio and MD Bates 《Clinical genetics》2009,75(5):429-439
Congenital diaphragmatic hernia (CDH) is a common birth defect for which few causative genes have been identified. Several candidate regions containing genes necessary for normal diaphragm development have been identified, including a 4–5 Mb deleted region at chromosome 1q41-1q42 from which the causative gene(s) has/have not been cloned. We selected the HLX gene from this interval as a candidate gene for CDH, as the Hlx homozygous null mouse has been reported to have diaphragmatic defects and the gene was described as being expressed in the murine diaphragm. We re-sequenced HLX in 119 CDH patients and identified four novel single nucleotide substitutions that predict amino acid changes: p.S12F, p.S18L, p.D173Y and p.A235V. These sequence alterations were all present in patients with isolated CDH, although patients with both isolated CHD and CDH with additional anomalies were studied. The single-nucleotide substitutions were absent in more than 186 control chromosomes. In-situ hybridization studies confirmed expression of Hlx in the developing murine diaphragm at the site of the junction of the diaphragm and the liver. Although functional studies to determine if these novel sequence variants altered the inductive activity of Hlx on the α-smooth muscle actin and SM22α promoters showed no significant differences between the variants and wild-type Hlx , sequence variants in HLX may still be relevant in the pathogenesis of CDH in combination with additional genetic and environmental factors. 相似文献
993.
ALPM Catelani ACV Krepischi CA Kim F Kok PA Otto MTBM Auricchio JF Mazzeu DT Uehara SS Costa J Knijnenburg A Tabith Jr AM Vianna-Morgante RC Mingroni-Netto C Rosenberg 《Clinical genetics》2009,76(5):458-464
The cause of hearing impairment has not been elucidated in a large proportion of patients. We screened by 1-Mb array-based comparative genomic hybridization (aCGH) 29 individuals with syndromic hearing impairment whose clinical features were not typical of known disorders. Rare chromosomal copy number changes were detected in eight patients, four de novo imbalances and four inherited from a normal parent. The de novo alterations define candidate chromosome segments likely to harbor dosage-sensitive genes related to hearing impairment, namely 1q23.3–q25.2, 2q22q23, 6p25.3 and 11q13.2–q13.4. The rare imbalances also present in normal parents might be casually associated with hearing impairment, but its role as a predisposition gene remains a possibility. Our results show that syndromic deafness is frequently associated with chromosome microimbalances (14–27%), and the use of aCGH for defining disease etiology is recommended. 相似文献
994.
Duong Thuy Tran BMed MIPH rea M. Stone RN MHS MAppMan Ritin S. Fernandez RN MN PhD Rhonda D. Griffiths AM RN RM BEd MSc DrPH FRCNA Maree Johnson RN BAppSci MAppSci PhD 《Perspectives in psychiatric care》2009,45(2):128-139
PURPOSE. This study aims to assess the impact of an education program on Australian general nurses' knowledge and competence in identifying and managing patients with alcohol and substance use and misuse, and compare findings with existing literature on mental health nurses.
DESIGN AND METHODS. Pre- and posttest design without a control group.
FINDINGS. The nurses' knowledge of safe drinking limits and alcohol withdrawal management improved following the education. Nevertheless, overall the nurses reported a lack of adequate knowledge and competence. Compared to mental health nurses, the nurses in this study had lower levels of knowledge and competence.
PRACTICE IMPLICATIONS. We suggest several opportunities for general nurses to strengthen their knowledge and skills and the need for a comprehensive and regular education program. 相似文献
DESIGN AND METHODS. Pre- and posttest design without a control group.
FINDINGS. The nurses' knowledge of safe drinking limits and alcohol withdrawal management improved following the education. Nevertheless, overall the nurses reported a lack of adequate knowledge and competence. Compared to mental health nurses, the nurses in this study had lower levels of knowledge and competence.
PRACTICE IMPLICATIONS. We suggest several opportunities for general nurses to strengthen their knowledge and skills and the need for a comprehensive and regular education program. 相似文献
995.
C Severini G La Corte G Improta M Broccardo S Agostini C Petrella V Sibilia F Pagani F Guidobono I Bulgarelli GL Ferri C Brancia AM Rinaldi A Levi R Possenti 《British journal of pharmacology》2009,157(6):984-993
Background and purpose:
Vgf gene expression has been detected in various endocrine and neuronal cells in the gastrointestinal tract. In this study we investigated the pharmacological activity of different VGF-derived peptides. Among these, TLQP-21, corresponding to the 556–576 fragment of the protein was the unique active peptide, and its pharmacological profile was further studied.Experimental approach:
The effects of TLQP-21 were examined in vitro by smooth muscle contraction in isolated preparations from the rat gastrointestinal tract and, in vivo, by assessing gastric emptying in rats. Rat stomach tissues were also processed for immunohistochemical and biochemical characterization.Key results:
In rat longitudinal forestomach strips, TLQP-21 (100 nmol·L−1–10 µmol·L−1) concentration-dependently induced muscle contraction (in female rats, EC50 = 0.47 µmol·L−1, Emax: 85.7 ± 7.9 and in male rats, 0.87 µmol·L−1, Emax: 33.4 ± 5.3; n = 8), by release of prostaglandin (PG)E2 and PGF2a from the mucosal layer. This effect was significantly antagonized by indomethacin and selective inhibitors of either cyclooxygenase-1 (S560) or cyclooxygenase-2 (NS398). Immunostaining and biochemical studies confirmed the presence of VGF in the gastric neuronal cells. TLQP-21, injected i.c.v. (2–32 nmol per rat), significantly decreased gastric emptying by about 40%. This effect was significantly (P < 0.05) blocked by i.c.v. injection of indomethacin, suggesting that, also in vivo, this peptide acts in the brain stimulating PG release.Conclusions and implications:
The present results demonstrate that this VGF-derived peptide plays a central and local role in the regulation of rat gastric motor functions. 相似文献996.
Background
Lipid-lowering medication remains underused, even in high-risk populations. The objective of this study was to determine factors underlying general practitioners' decisions not to prescribe such drugs to patients with type 2 diabetes.Methods
A qualitative study with semi-structured interviews using real cases was conducted to explore reasons for not prescribing lipid-lowering medication after a guideline was distributed that recommended the use of statins in most patients with type 2 diabetes. Seven interviews were conducted with general practitioners (GPs) in The Netherlands, and analysed using an analytic inductive approach.Results
Reasons for not-prescribing could be divided into patient and physician-attributed factors. According to the GPs, some patients do not follow-up on agreed medication and others object to taking lipid-lowering medication, partly for legitimate reasons such as expected or perceived side effects. Furthermore, the GPs themselves perceived reservations for prescribing lipid-lowering medication in patients with short life expectancy, expected compliance problems or near goal lipid levels. GPs sometimes postponed the start of treatment because of other priorities. Finally, barriers were seen in the GPs' practice organisation, and at the primary-secondary care interface.Conclusion
Some of the barriers mentioned by GPs seem to be valid reasons, showing that guideline non-adherence can be quite rational. On the other hand, treatment quality could improve by addressing issues, such as lack of knowledge or motivation of both the patient and the GP. More structured management in general practice may also lead to better treatment. 相似文献997.
998.
Rachel Saunders‐Pullman MD MPH MS Johann Hagenah MD Vijay Dhawan MD Kaili Stanley BS Gregory Pastores MD Swati Sathe MD Michele Tagliati MD Kelly Condefer MD Christina Palmese PhD Norbert Brüggemann MD Christine Klein MD AM Roe MD Ruth Kornreich PhD Laurie Ozelius PhD Susan Bressman MD 《Movement disorders》2010,25(10):1364-1372
Among the genes implicated for parkinsonism is glucocerebrosidase (GBA), which causes Gaucher disease (GD). Despite a growing literature that GD may present as parkinsonism, neuroimaging, olfaction, and neuropsychological testing have not been extensively reported. We describe transcranial sonography (TCS), 18F‐fluorodopa (F‐dopa) and fluorodeoxyglucose (FDG) Positron emission tomography, olfaction testing, neuropsychological testing, and clinical features in homozygous and compound heterozygous GBA mutation carriers identified through screening of 250 Ashkenazi Jewish parkinsonian individuals treated at a tertiary care center. We identified two individuals with N370S/R496H compound heterozygous mutations and two with N370S homozygous mutations; one individual died before completing detailed evaluation. TCS (n = 3) demonstrated nigral hyperechogenicity that was greater than controls [median area maximal substantia nigra echogenicity (aSNmax) = 0.28 cm2 vs. 0.14 cm2, P = 0.005], but similar to idiopathic PD (aSNmax = 0.31 cm2). FDG PET (n = 2) demonstrated hypermetabolism of the lentiform nuclei, and F‐fluorodopa PET (n = 2), bilateral reduction in striatal F‐dopa uptake. Olfaction was markedly impaired in the two tested cases, including onset of smell disturbance in adolescence in one. Neuropsychological features (n = 3) were consistent with Parkinson's disease (PD) or diffuse Lewy body disease (DLB). The imaging, neuropsychological and olfactory markers suggest the GD phenotype includes PD with and without features of DLB, marked olfactory loss, nigral hyperechogenicity on TCS, and F‐dopa and FDG PET abnormalities. © 2010 Movement Disorder Society 相似文献
999.
E Christensen T Warloe S Kroon J Funk P Helsing AM Soler HJ Stang Ø Vatne C Mørk 《Journal of the European Academy of Dermatology and Venereology》2010,24(5):505-512
Methyl aminolaevulinate photodynamic therapy is increasingly practiced in the treatment of actinic keratoses, Bowen’s disease and basal cell carcinomas. This method is particularly suitable for treating multiple lesions, field cancerization and lesions in areas where a good cosmetic outcome is of importance. Good treatment routines will contribute to a favourable result. The Norwegian photodynamic therapy (PDT) group consists of medical specialists with long and extensive PDT experience. With support in the literature, this group presents guidelines for the practical use of topical PDT in non‐melanoma skin cancer. 相似文献