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排序方式: 共有166条查询结果,搜索用时 15 毫秒
71.
Human megakaryocytes express clusterin and package it without apolipoprotein A-1 into alpha-granules 总被引:1,自引:0,他引:1
Tschopp J; Jenne DE; Hertig S; Preissner KT; Morgenstern H; Sapino AP; French L 《Blood》1993,82(1):118-125
Clusterin, a 70-Kd disulfide-linked two-chain plasma glycoprotein circulates in blood as a high-density lipoprotein particle and is highly induced after tissue injury and tissue remodeling. In this study, peripheral blood leukocytes were assayed for clusterin expression. The protein was predominantly detectable in human platelets by immune cytochemistry. The content of clusterin was determined and amounts to 2.5 +/- 1.3 micrograms/10(9) platelets, thus representing about 2% of the blood pool. Clusterin purified from human platelets had the same molecular weight as plasma clusterin under nonreducing conditions and was composed of two disulfide-linked nonidentical subunits of the same size. Both preparations were sensitive to reduction yielding the two subunits of 35 Kd. In contrast to plasma clusterin, the platelet form was not complexed to apolipoprotein A-I. By immunogold labeling, alpha-granule localization of clusterin was observed. Complete release of platelet clusterin occurred at optimal doses of A23187, phorbol myristate acetate (PMA), and thrombin. Because clusterin mRNA was detected by hybridization in situ in bone marrow- derived megakaryocytes, platelet clusterin is most likely produced and packaged into alpha-granules during megakaryocyte development. 相似文献
72.
Posttransplant T-cell lymphoproliferative disorders--an aggressive, late complication of solid-organ transplantation 总被引:6,自引:0,他引:6
Hanson MN; Morrison VA; Peterson BA; Stieglbauer KT; Kubic VL; McCormick SR; McGlennen RC; Manivel JC; Brunning RD; Litz CE 《Blood》1996,88(9):3626-3633
T-cell non-Hodgkin's lymphomas are an uncommon occurrence after solid- organ transplantation. We describe a morphologically and immunophenotypically distinct group of T-cell lymphoproliferative disorders that occurred late in the course of six patients with solid- organ transplants. The patients ranged in age from 31 to 56 years (median, 43). Three were male; all were splenectomized. The interval from transplant to the diagnosis of lymphoma ranged from 4 to 26 years (median, 15). Symptoms at presentation were related to sites of involvement. Pulmonary, marrow, and CNS involvement were present in five, four, and one case, respectively. No patient had lymphadenopathy. Five patients had an elevated lactate dehydrogenase level (range, 226 to 4,880 IU/L; median, 1,220 IU/L). Five of six patients had a leukoerythroblastic reaction. All cases had large-cell histology and frequently contained cytoplasmic granules. Those cases tested expressed CD2, CD3, and CD8 and were negative for B-cell antigens. T-cell receptor beta- and gamma-chain genes were clonally rearranged in three of three and one of three cases, respectively. All T-cell posttransplant lymphoproliferative disorders (T-PTLDs) studied were negative for Epstein-Barr virus (EBV), human T-cell leukemia/lymphoma virus type 1 (HTLV-1), human T-cell leukemia/lymphoma virus type 2 (HTLV-2), and human herpes virus type 8 (HHV-8) genomes. Treatment with acyclovir (three patients) or chemotherapy (three patients) resulted in two responses. All patients had an aggressive course, with a median survival duration of 5 weeks. In conclusion, a clinically aggressive T- PTLD may be a late complication of solid-organ transplantation and does not appear to be related to EBV, HTLV-1, HTLV-2, or HHV-8 infection. 相似文献
73.
CAS Garbin AJI Garbin KT dos Santos M de Lourdes Carvalho DC Lima 《International journal of dental hygiene》2009,7(4):285-288
Abstract: The toothbrush is an effective instrument for oral diseases prevention. The flexibility and status of bristles, as well as the size of the handle are fundamental for an effective toothbrushing. Thus, the assessment of physical characteristics of toothbrushes is important. This study evaluated the deterioration and wear of bristles of toothbrushes used by preschool children. For the sample selection, five elementary schools were assorted from a city of the São Paulo State northwest region, one from each city area. All toothbrushes used by preschool children who aged between 5 and 6 years old and who attended the assorted schools were visually analysed by two calibrated examiners, according to the criteria proposed by Rawls HR, Mkwayi-Tulloch NJ, Casella R, Cosgrove R ( J Dent Res 1989; 12:1781): 0 – it is impossible to state if the toothbrush was used or not; 1 – the bristles seem to be separated within some tufts; 2 – most tufts are separated, many cover other tufts and present a large number of curved and inclined bristles; 3 – most tufts are covered by others and bristles are folded and tipped. In total, 333 toothbrushes were evaluated. The data obtained revealed that 57.96% of the toothbrushes presented adequate condition for utilization (scores 0 and 1), whereas 42.04% presented inadequate bristles for their function (scores 2 and 3). It was concluded that a great number of toothbrushes presented deterioration of the bristles. Thus, there is the need to guide and promote awareness among teachers, parents and children as well as the need to replace toothbrushes. 相似文献
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75.
U Jappe†‡ R Boit† MD Farrar† E Ingham† J Sandoe† KT Holland† 《Journal of the European Academy of Dermatology and Venereology》2004,18(4):450-454
BACKGROUND: Propionibacterium acnes is primarily associated with the pathogenesis of acne vulgaris but reports are increasing in number implicating P. acnes in other diseases such as abscess formation, meningitis and endocarditis. The pathogenicity of P. acnes is thought to be partly due to the interaction of the bacterium with the immune system. Historically, investigations have focused on humoral and cell-mediated immune responses to P. acnes antigens without attention to the possibility that different antigens may be expressed by different isolates. OBJECTIVE: Investigations were performed to determine whether there were differences between a laboratory strain of P. acnes (P-37) and fresh clinical isolates in their ability to stimulate naive and adult lymphocytes. MATERIAL AND METHODS: The fresh isolates were collected from a patient with inflammatory acne and a patient with P. acnes-induced prosthetic valve endocarditis. The lymphocyte transformation assay was used to detect responses to whole-cell suspensions of stationary phase P. acnes isolates during 7 days of incubation. RESULTS: The acne isolate was significantly more stimulatory for cord blood mononuclear cells (CBMNCs) than the laboratory isolate (P. acnes P-37) at day 4 of incubation. There were no significant differences between the three strains at any other time points. However, the isolate cultivated from inflammatory acne was significantly more stimulatory for peripheral blood mononuclear cells (PBMNCs) from acne donors than the endocarditis isolate or the laboratory strain at most time points. There were no significant differences between the endocarditis strain and the laboratory strain. CONCLUSION: It can be hypothesized that in case of P. acnes-induced endocarditis lymphocyte stimulation is a disadvantage for the microorganism and therefore a lack of lymphocyte stimulation may be relevant to the pathogenesis of endocarditis. 相似文献
76.
Gene deletion at the glutathione S-transferase mu locus (GSTM1) has
previously been associated with increased risk for environmentally- induced
cancers (e.g. smoking-related lung cancer). In the present study we
examined the hypothesis that GSTM1 deletion is a risk factor for malignant
brain tumors in adults. We compared the prevalence of the GSTM1 homozygous
deletion polymorphism in 158 Caucasian adults with gliomas with 157
controls. Cases and controls were drawn from a large population-based
case-control study of brain cancers in six San Francisco Bay area counties.
Overall, the prevalence of the GSTM1 deletion was similar in cases (83/158;
53%) and controls (78/157; 50%). Among brain tumor cases, analysis of
variance modeling indicated a significant interaction of GSTM1 genotype and
gender associated with age at diagnosis (P = 0.02). This effect was due to
the fact that women with GSTM1 deletion were younger on average at
diagnosis than women who were GSTM1 positive (43.9 years versus 52.4 years,
respectively). Age at diagnosis among men was similar for those who were
GSTM1 deleted and GSTM1 positive (49.4 years and 47.2 years, respectively).
The younger age at diagnosis of GSTM1 null female cases compared with GSTM1
positive cases was observed in astrocytoma as well as the higher grade
tumors (e.g. glioblastoma multiforme). There was no association of GSTM1
deletion with age or gender in controls. These studies suggest that among
female cases, GSTM1 deletion may be associated with earlier age at onset.
Confirmation of these findings could provide important clues to
gene-environment interactions in the etiology of malignant brain tumors.
相似文献
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Riitta KT. Saarela MSc Helena Soini DSc Seija Muurinen DSc Merja H. Suominen DSc Kaisu H. Pitkälä MD PhD 《Special care in dentistry》2013,33(2):56-61
The aim of this study was to determine the associations of older assisted living residents’ tooth brushing habits with health and nutritional status. We assessed the tooth brushing habits, nutritional status, oral health, use of dental services, and morbidity of 1,447 assisted living residents in the Helsinki metropolitan area of Finland. Of the residents, 17% did not clean or had not cleaned their teeth and/or dentures daily. Those not cleaning their teeth and/or dentures daily were more often male, less educated and had a mean length of stay in assisted living longer than those who cleaned their teeth and/or dentures daily. They were more often malnourished and dependent in ADLs. In addition they more often had poorer oral health and used fewer dental services. Poor tooth brushing habits indicate poor oral and subjective health. More attention should focus on the oral hygiene of frail older assisted living residents. 相似文献