收费全文 | 10704篇 |
免费 | 482篇 |
国内免费 | 71篇 |
耳鼻咽喉 | 383篇 |
儿科学 | 445篇 |
妇产科学 | 520篇 |
基础医学 | 796篇 |
口腔科学 | 401篇 |
临床医学 | 918篇 |
内科学 | 2581篇 |
皮肤病学 | 179篇 |
神经病学 | 516篇 |
特种医学 | 459篇 |
外科学 | 2602篇 |
综合类 | 158篇 |
一般理论 | 2篇 |
预防医学 | 230篇 |
眼科学 | 358篇 |
药学 | 377篇 |
中国医学 | 27篇 |
肿瘤学 | 305篇 |
2023年 | 80篇 |
2022年 | 96篇 |
2021年 | 312篇 |
2020年 | 161篇 |
2019年 | 209篇 |
2018年 | 261篇 |
2017年 | 209篇 |
2016年 | 347篇 |
2015年 | 415篇 |
2014年 | 540篇 |
2013年 | 624篇 |
2012年 | 796篇 |
2011年 | 737篇 |
2010年 | 485篇 |
2009年 | 491篇 |
2008年 | 771篇 |
2007年 | 853篇 |
2006年 | 754篇 |
2005年 | 740篇 |
2004年 | 644篇 |
2003年 | 543篇 |
2002年 | 436篇 |
2001年 | 84篇 |
2000年 | 82篇 |
1999年 | 59篇 |
1998年 | 48篇 |
1997年 | 43篇 |
1996年 | 50篇 |
1995年 | 38篇 |
1994年 | 23篇 |
1993年 | 13篇 |
1992年 | 35篇 |
1991年 | 28篇 |
1990年 | 18篇 |
1989年 | 14篇 |
1988年 | 12篇 |
1987年 | 16篇 |
1986年 | 14篇 |
1985年 | 17篇 |
1984年 | 15篇 |
1983年 | 16篇 |
1981年 | 11篇 |
1980年 | 8篇 |
1979年 | 13篇 |
1975年 | 9篇 |
1973年 | 12篇 |
1970年 | 8篇 |
1969年 | 10篇 |
1968年 | 6篇 |
1966年 | 6篇 |
AIM
To investigate the association of serum glucocorticoid kinase gene-1 (SGK-1) DNA variants with chronic central serous chorioretinopathy (CSC).METHODS
We enrolled 32 eyes of 32 patients who were diagnosed with chronic CSC and composed 32 normal eyes as a control group. Peripheral blood was used for DNA extraction and polymerase chain reaction (PCR) amplification. SGK1 gene was sequenced by using BigDye® Terminator v3.1 cycle sequencing KIT (Applied Biosystems, Foster City, CA, USA). The SGK1 gene and its variants were investigated in CSC patient group and control group.RESULTS
We identified a new polymorphism M32V in two person in the patient group (Minor allele frequency (MAF)=0.009) on the region of 1-60 amino acids. The rs1057293 was located in the encoder region of the SGK 1 gene but not associated with CSC (P=0.68). An intrinsic rs1743966 is also not associated (P=0.28).CONCLUSIONS
The new polymorphism M32V is located on the region of 1-60 amino acids which is necessary for localization to the mitochondria in CSC patient. This mutation is probably important for the energy metabolism and plays an important role in the cellular response to hyperosmotic stress and other stress stimuli. Both rs1057293 and rs1743966 are not associated with CSC. 相似文献Antiphospholipid syndrome (APS) is a systemic autoimmune disorder characterized by arterial and/ or venous thrombosis accompanied by persistently elevated levels of antiphospholipid antibodies (aPLs). The aim of this study is to evaluate the pulmonary manifestations of APS and compare the levels of aPLs in patients with and without pulmonary involvement. We retrospectively reviewed the files of patients with the diagnosis of APS between October 2010 and May 2017. Demographic data, clinical, radiological and laboratory findings were recorded. The study included 67 patients (56 female/11 male) with a mean age of 39?±?13 years. Pulmonary manifestations such as parenchymal and/or vascular involvement were seen in 12 (17.9%) patients. The patients with and without pulmonary manifestations were not significantly different in terms of age (p?=?0.46), comorbidities (p?=?0.48) and APS duration (p?=?0.66). Acute pulmonary thromboembolism (PE) was determined in 11 (16.4%), alveolar hemorrhage in 2 (3%) patients. Four patients with acute PE (36%) developed chronic thromboembolic pulmonary hypertension (CTEPH). One patient developed both CTEPH and diffuse alveolar hemorrhage after acute PE during follow up. Antiphosholipid antibody IgM was highly positive in patients with PE compared to patients without PE (p?=?0.005). Other antibodies and lupus anticoagulant were not significantly different in patients with and without PE. None of the patients were deceased due to pulmonary manifestations of APS. PE was the most common pulmonary manifestation of APS. The development of CTEPH was high among APS patients. Patients with APS should be closely followed for the onset of PE and CTEPH.
相似文献