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991.
992.
Camille Guillot Jackie Badcock Katie Clow Jennifer Cram Shaun Dergousoff Antonia Dibernardo Michelle Evason Erin Fraser Eleni Galanis Salima Gasmi Greg J German Douglas T Howse Claire Jardine Emily Jenkins Jules Koffi Manisha Kulkarni L Robbin Lindsay Genevieve Lumsden Roman McKay Kieran Moore Muhammad Morshed Douglas Munn Mark Nelder Joe Nocera Marion Ripoche Kateryn Rochon Curtis Russell Andreea Slatculescu Benoit Talbot Karine Thivierge Maarten Voordouw Catherine Bouchard Patrick Leighton 《传染性疾病的发现RMTC(加拿大)》2020,46(10):354
BackgroundLyme disease is an emerging vector-borne zoonotic disease of increasing public health importance in Canada. As part of its mandate, the Canadian Lyme Disease Research Network (CLyDRN) launched a pan-Canadian sentinel surveillance initiative, the Canadian Lyme Sentinel Network (CaLSeN), in 2019.ObjectivesTo create a standardized, national sentinel surveillance network providing a real-time portrait of the evolving environmental risk of Lyme disease in each province.MethodsA multicriteria decision analysis (MCDA) approach was used in the selection of sentinel regions. Within each sentinel region, a systematic drag sampling protocol was performed in selected sampling sites. Ticks collected during these active surveillance visits were identified to species, and Ixodes spp. ticks were tested for infection with Borrelia burgdorferi, Borrelia miyamotoi, Anaplasma phagocytophilum, Babesia microti and Powassan virus.ResultsIn 2019, a total of 567 Ixodes spp. ticks (I. scapularis [n=550]; I. pacificus [n=10]; and I. angustus [n=7]) were collected in seven provinces: British Columbia, Manitoba, Ontario, Québec, New Brunswick, Nova Scotia and Prince Edward Island. The highest mean tick densities (nymphs/100 m2) were found in sentinel regions of Lunenburg (0.45), Montréal (0.43) and Granby (0.38). Overall, the Borrelia burgdorferi prevalence in ticks was 25.2% (0%–45.0%). One I. angustus nymph from British Columbia was positive for Babesia microti, a first for the province. The deer tick lineage of Powassan virus was detected in one adult I. scapularis in Nova Scotia.ConclusionCaLSeN provides the first coordinated national active surveillance initiative for tick-borne disease in Canada. Through multidisciplinary collaborations between experts in each province, the pilot year was successful in establishing a baseline for Lyme disease risk across the country, allowing future trends to be detected and studied. 相似文献
993.
994.
14q deletions are associated with trisomy 12, NOTCH1 mutations and unmutated IGHV genes in chronic lymphocytic leukemia and small lymphocytic lymphoma
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Adrien Cosson Elise Chapiro Nabila Belhouachi Hong‐Anh Cung Boris Keren Frederik Damm Caroline Algrin Christine Lefebvre Sandra Fert‐Ferrer Isabelle Luquet Nathalie Gachard Francine Mugneret Christine Terre Marie‐Agnes Collonge‐Rame Lucienne Michaux Isabelle Rafdord‐Weiss Pascaline Talmant Lauren Veronese Nathalie Nadal Stephanie Struski Carole Barin Catherine Helias Marina Lafage Eric Lippert Nathalie Auger Virginie Eclache Damien Roos‐Weil Veronique Leblond Catherine Settegrana Karim Maloum Frederic Davi Helene Merle‐Beral Claude Lesty Florence Nguyen‐Khac 《Genes, chromosomes & cancer》2014,53(8):657-666
Deletions of the long arm of chromosome 14 [del(14q)] are rare but recurrently observed in mature B‐cell neoplasms, particularly in chronic lymphocytic leukemia (CLL). To further characterize this aberration, we studied 81 cases with del(14q): 54 of CLL and 27 of small lymphocytic lymphoma (SLL), the largest reported series to date. Using karyotype and fluorescence in situ hybridization (FISH), the most frequent additional abnormality was trisomy 12 (tri12), observed in 28/79 (35%) cases, followed by del13q14 (12/79, 15%), delTP53 (11/80, 14%) delATM (5/79, 6%), and del6q21 (3/76, 4%). IGHV genes were unmutated in 41/53 (77%) patients, with a high frequency of IGHV1‐69 (21/52, 40%). NOTCH1 gene was mutated in 14/45 (31%) patients. There was no significant difference in cytogenetic and molecular abnormalities between CLL and SLL. Investigations using FISH and SNP‐array demonstrated the heterogeneous size of the 14q deletions. However, a group with the same del(14)(q24.1q32.33) was identified in 48% of cases. In this group, tri12 (P = 0.004) and NOTCH1 mutations (P = 0.02) were significantly more frequent than in the other patients. In CLL patients with del(14q), median treatment‐free survival (TFS) was 27 months. In conclusion, del(14q) is associated with tri12 and with pejorative prognostic factors: unmutated IGHV genes (with over‐representation of the IGHV1‐69 repertoire), NOTCH1 mutations, and a short TFS. © 2014 Wiley Periodicals, Inc. 相似文献
995.
Pauline Penot Arsène Héma Guillaume Bado Firmin Kaboré Ibrahim Soré Diamasso Sombié Jean-Richard Traoré Jean-Baptiste Guiard-Schmid Arnaud Fontanet Laurence Slama Adrien Bruno Sawadogo Christian Laurent 《Journal of the International AIDS Society》2014,17(1)
Introduction
Gender differences in antiretroviral therapy (ART) outcomes are critical in sub-Saharan Africa. We assessed the association between gender and virologic failure among adult patients treated in a public routine clinic (one of the largest in West Africa) in Burkina Faso.Methods
We performed a case-control study between July and October 2012 among patients who had received ART at the Bobo Dioulasso Day Care Unit. Patients were eligible if they were 15 years or older, positive for HIV-1 or HIV-1+2, and on first-line ART for at least six months. Cases were all patients with two consecutive HIV loads >1000 copies/mL (Biocentric Generic or Abbott Real Time assays), or one HIV load >1000 copies/mL associated with immunologic or clinical failure criteria. Controls were all patients who only had HIV loads <300 copies/mL. The association between gender and virologic failure was assessed using a multivariate logistic regression, adjusted on age, level of education, baseline CD4+ T cell count, first and current antiretroviral regimens and time on ART.Results
Of 2303 patients (74.2% women; median age: 40 years; median time on ART: 34 months), 172 had virologic failure and 2131 had virologic success. Among the former, 130 (75.6%) had confirmed virologic failure, 38 (22.1%) had viro-immunologic failure, and four (2.3%) had viro-clinical failure. The proportion of men was significantly higher among the cases than among the controls (37.2% vs. 24.9%; p<0.001). Compared to controls, cases were also younger, more immunodeficient at ART initiation, less likely to receive a protease inhibitor-based antiretroviral regimen and had spent a longer period of time on ART. After adjustment, male gender remained strongly associated with virologic failure (odds ratio 2.52, 95% CI: 1.77–3.60; p<0.001).Conclusions
Men on ART appeared more vulnerable to virologic failure than women. Additional studies are needed to confirm the poorer prognosis of men in this setting and to determine the causes for their vulnerability in order to optimize HIV care. From now on, efforts should be made to support the adherence of men to ART in the African setting. 相似文献996.
Bedoui Yosra Lebeau Grégorie Guillot Xavier Dargai Farouk Guiraud Pascale Neal Jim W. Ralandison Stéphane Gasque Philippe 《Journal of neuroimmune pharmacology》2020,15(4):838-851
Journal of Neuroimmune Pharmacology - In contrast to the significant advances in our understanding of the mesenchymal stem cell (MSC) populations in bone marrow (BM), little is known about the MSCs... 相似文献
997.
998.
Andrew M. Borman Adrien Szekely Chistopher J. Linton Michael D. Palmer Phillipa Brown Elizabeth M. Johnson 《Journal of clinical microbiology》2013,51(3):967-972
Candida africana was previously proposed as a new species within the Candida albicans species complex, together with C. albicans and C. dubliniensis, although further phylogenetic analyses better support its status as an unusual variant within C. albicans. Here we show that C. africana can be distinguished from C. albicans and C. dubliniensis by pyrosequencing of a short region of ITS2, and we have evaluated its occurrence in clinical samples by pyrosequencing all presumptive isolates of C. albicans submitted to the Mycology Reference Laboratory over a 9-month period. The C. albicans complex constituted 826/1,839 (44.9%) of yeast isolates received over the study period and included 783 isolates of C. albicans, 28 isolates of C. dubliniensis, and 15 isolates of C. africana. In agreement with previous reports, C. africana was isolated exclusively from genital specimens, in women in the 18-to-35-year age group. Indeed, C. africana constituted 15/251 (6%) of “C. albicans” isolates from female genital specimens during the study period. C. africana isolates were germ tube positive, grew significantly more slowly than C. albicans and C. dubliniensis on conventional mycological media, could be distinguished from the other members of the C. albicans complex by appearance on chromogenic agar, and were incapable of forming chlamydospores. Here we present the detailed evaluation of epidemiological, phenotypic, and clinical features and antifungal susceptibility profiles of United Kingdom isolates of C. africana. Furthermore, we demonstrate that C. africana is significantly less pathogenic than C. albicans and C. dubliniensis in the Galleria mellonella insect systemic infection model. 相似文献
999.
1000.
Julie Caron Alain Mangé Bernard Guillot Jérôme Solassol 《Journal of cancer research and clinical oncology》2009,135(9):1257-1264
Purpose There is no available tumor marker that can detect primary melanoma. Proteomics analysis has been proposed as a novel tool
that would lead to the discovery of potential new tumor markers.
Methods We developed a serum proteomic fingerprinting approach coupled with a classification method to determine whether proteomic
profiling could discriminate between melanoma and healthy volunteers. A total of 108 serum samples from 30 early-stage [American
Joint Committee on Cancer (AJCC) stage I or II] and 30 advanced-stage (AJCC stage III or IV) melanoma patients and 48 healthy
volunteers were analyzed by surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS) utilizing
protein chip technology and artificial neural networks.
Results In a first step, a multiprotein classifier was built using a training set of 30 pathologically confirmed melanoma and 24 healthy
volunteer serum samples, resulting in good classification accuracy for correct diagnosis and stage classification assignment.
Subsequently, our multiprotein classifier was tested in an independent validation set of 30 melanoma and 24 non-cancer serum
samples patients, maintained in a good diagnostic accuracy of 98.1% (sensitivity 96.7%, specificity 100%), and 100% stage
I/II classification assignment.
Conclusions Although results remain to be confirmed in larger collective patient cohorts, we could demonstrate the usefulness of proteomic
profiling as a sensitive and specific assay to detect melanoma, including non-metastatic melanoma, from the serum.
J. Caron and A. Mangé contributed equally to this work. 相似文献