全文获取类型
收费全文 | 113篇 |
免费 | 10篇 |
国内免费 | 17篇 |
专业分类
儿科学 | 6篇 |
妇产科学 | 4篇 |
基础医学 | 23篇 |
口腔科学 | 1篇 |
临床医学 | 28篇 |
内科学 | 22篇 |
皮肤病学 | 1篇 |
神经病学 | 2篇 |
特种医学 | 3篇 |
外科学 | 9篇 |
综合类 | 2篇 |
预防医学 | 14篇 |
眼科学 | 7篇 |
药学 | 10篇 |
肿瘤学 | 8篇 |
出版年
2023年 | 1篇 |
2022年 | 1篇 |
2021年 | 7篇 |
2020年 | 2篇 |
2019年 | 2篇 |
2018年 | 2篇 |
2017年 | 2篇 |
2016年 | 2篇 |
2015年 | 3篇 |
2014年 | 1篇 |
2013年 | 3篇 |
2012年 | 2篇 |
2011年 | 12篇 |
2010年 | 4篇 |
2009年 | 5篇 |
2008年 | 4篇 |
2007年 | 13篇 |
2006年 | 3篇 |
2005年 | 5篇 |
2004年 | 2篇 |
2003年 | 1篇 |
2002年 | 4篇 |
2001年 | 6篇 |
1999年 | 5篇 |
1998年 | 5篇 |
1997年 | 7篇 |
1996年 | 10篇 |
1995年 | 3篇 |
1994年 | 3篇 |
1993年 | 7篇 |
1992年 | 1篇 |
1991年 | 2篇 |
1989年 | 1篇 |
1988年 | 1篇 |
1987年 | 3篇 |
1985年 | 1篇 |
1981年 | 1篇 |
1978年 | 1篇 |
1958年 | 1篇 |
1954年 | 1篇 |
排序方式: 共有140条查询结果,搜索用时 13 毫秒
21.
Bréart B Ramos-Perez WD Mendoza A Salous AK Gobert M Huang Y Adams RH Lafaille JJ Escalante-Alcalde D Morris AJ Schwab SR 《The Journal of experimental medicine》2011,208(6):1267-1278
The signaling lipid sphingosine-1-phosphate (S1P) stabilizes the vasculature, directs lymphocyte egress from lymphoid organs, and shapes inflammatory responses. However, little is known about how S1P distribution is controlled in vivo, and it is not clear how a ubiquitously made lipid functions as a signal that requires precise spatial and temporal control. We have found that lipid phosphate phosphatase 3 (LPP3) enables efficient export of mature T cells from the thymus into circulation, and several lines of evidence suggest that LPP3 promotes exit by destroying thymic S1P. Although five additional S1P-degrading enzymes are expressed in the thymus, they cannot compensate for the loss of LPP3. Moreover, conditional deletion of LPP3 in either epithelial cells or endothelial cells is sufficient to inhibit egress. These results suggest that S1P generation and destruction are tightly regulated and that LPP3 is essential to establish the balance. 相似文献
22.
El Saman AM Saad H Hassanin AI Shokery M Abdelghaffar HM 《American journal of obstetrics and gynecology》2012,206(3):267.e1-267.e4
Inverted balloon vaginoplasty technique was performed for a case with segmental vaginal aplasia and upper hematocolpos within 50 min operative time without any complications. The neovagina was naturally covered, 11 cm in depth and communicated with dimple at hymen's site. Regular painless menstruations were reported. 相似文献
23.
24.
LW Law TK Lau TY Fung TY Leung CC Wang KW Choy 《BJOG : an international journal of obstetrics and gynaecology》2009,116(2):339-343
Objective We investigated the application of high-resolution microarray-based comparative genomic hybridisation (array CGH) on a fetus showing increased nuchal translucency (NT).
Design Case study.
Setting Tertiary referral obstetrics unit.
Sample Pregnant woman attended the antenatal clinic.
Methods Conventional karyotyping and genetic test was carried out for the alpha-globin gene. High-resolution array CGH using the high-density 244K Agilent microarray was performed on fetal blood sample by cordocentesis to investigate the possibility of any genomic imbalance.
Main outcome measures Detection of chromosomal abnormality.
Results Karyotyping analysis showed 46,XY. Molecular genetic diagnosis confirms the fetus has Hb-H constant spring disease but cannot explain the increased NT to 3.2 mm. Array CGH analysis discovered a 1.32-Mb microdeletion on chromosome 16p13.11. Deletion at 16p13.11 has been implicated to predispose to autism and/or mental retardation. Baby was delivered at 40 weeks of gestation, and follow up was carried out at 3 months of age without sign of mental retardation/developmental delay.
Conclusions This case study demonstrated that array CGH can accurately calibrate the size and identify de novo interstitial chromosome imbalances. However, the presence of chromosome copy variants with unknown clinical significance currently limits its wider scale application in prenatal diagnosis and needs further investigations. 相似文献
Design Case study.
Setting Tertiary referral obstetrics unit.
Sample Pregnant woman attended the antenatal clinic.
Methods Conventional karyotyping and genetic test was carried out for the alpha-globin gene. High-resolution array CGH using the high-density 244K Agilent microarray was performed on fetal blood sample by cordocentesis to investigate the possibility of any genomic imbalance.
Main outcome measures Detection of chromosomal abnormality.
Results Karyotyping analysis showed 46,XY. Molecular genetic diagnosis confirms the fetus has Hb-H constant spring disease but cannot explain the increased NT to 3.2 mm. Array CGH analysis discovered a 1.32-Mb microdeletion on chromosome 16p13.11. Deletion at 16p13.11 has been implicated to predispose to autism and/or mental retardation. Baby was delivered at 40 weeks of gestation, and follow up was carried out at 3 months of age without sign of mental retardation/developmental delay.
Conclusions This case study demonstrated that array CGH can accurately calibrate the size and identify de novo interstitial chromosome imbalances. However, the presence of chromosome copy variants with unknown clinical significance currently limits its wider scale application in prenatal diagnosis and needs further investigations. 相似文献
25.
26.
Aim
To investigate whether participation in a clinical audit and education session would improve GP management of patients who smoke.Methods
GPs who participated in an associated smoking cessation research program were invited to complete a three-stage clinical audit. This process included a retrospective self-audit of smoking cessation management practices over the 6 months prior to commencing the study, attending a 2.5 hour education session about GP management of smoking cessation, and completion of a second retrospective self-audit 6 months later. Twenty-eight GPs completed the full audit and education process, providing information about their smoking cessation management with 1114 patients. The main outcome measure was changes in GP management of smoking cessation with patients across the audit period, as measured by the clinical audit tool.Results
The majority of GPs (57%) indicated that as a result of the audit process they had altered their approach to the management of patients who smoke. Quantitative analyses confirmed significant increases in various forms of evidence-based smoking cessation management practices to assist patients to quit, or maintain quitting across the audit period. However comparative analyses of patient data challenged these findings, suggesting that the clinical audit process had less impact on GP practice than suggested in GP's self-reported audit data.Conclusion
This study provides some support for the combined use of self-auditing, feedback and education to improve GP management of smoking cessation. However further research is warranted to examine GP- and patient-based reports of outcomes from clinical audit and other educational interventions. 相似文献27.
Sufficient CD4+ T cell help is very important in generating specific
cytotoxic T cell responses. The inadequate activation of tumor-specific Th
cells leads to failure of antitumor immunity. In general, each individual
consists of some primed Th cells responding to certain antigens. If these
tumor non-specific pre-primed Th cells can provide sufficient help, the
generation of tumor-specific T cells may be enhanced. In the present study,
we tested this hypothesis by cognating and reactivating pre-primed
ovalbumin (OVA)-specific Th cells with OVA- pulsed tumor cells which could
simultaneously present both OVA and tumor-associated antigen on the same
cell. We clearly demonstrated that immunization of OVA-sensitized mice with
OVA-pulsed P388 cells, but not unpulsed P388 cells, led to the induction of
P388-specific cytotoxicity and tumor resistance. Both CD4+ and CD8+
tumor-specific cytotoxic T cells were detected in vitro, but only CD8+ T
cells played the major effector role in preventing the growth of challenged
tumor in vivo. Taken together, our study demonstrated that the
immunogenicity of tumor cells can be enhanced effectively by cognating
pre-primed foreign antigen-specific Th cells with tumor cells. These
findings have potential implications in developing methods to control tumor
growth.
相似文献
28.
A case report of a boy who demonstrates features of an athlete's heart, associated with dilatation of the coronary artery, is presented. The importance of distinguishing this benign condition from pathologic causes such as cardiomyopathy, and risk of sudden death in these athletes is discussed. 相似文献
29.
Bassel M. Elfokery Sahar A. Tawfic Abdelrahman M. Abdelrahman Dina N. Abbas Ikramy M. Abdelghaffar 《Journal of the Egyptian National Cancer Institute》2018,30(1):27-31
Introduction
Acute ipsilateral shoulder pain (ISP) is a common complaint in patients after thoracotomy. The incidence ranges from 21% to 97%. Unfortunately, clinical studies did not put enough focus on ISP post thoracic surgery.Aim of the work
This study was designed to compare the effectiveness of suprascapular nerve block (SNB) and phrenic nerve infiltration (PNI) for controlling ISP.Patients and methods
One hundred and thirty-five lung cancer patients (135) scheduled for open-lung surgery were randomly allocated into three equal groups; control group: received thoracic epidural with general anesthesia, suprascapular group: (SNB) one hour before the operation with 10?ml bupivacaine plus thoracic epidural with general anesthesia and phrenic nerve group: (PNI) was performed by the operating surgeon with 10?ml bupivacaine plus thoracic epidural with general anesthesia.The visual analogue score (VAS) of ISP, rescue of ketorolac for break through shoulder pain, peak expiratory flow rate (PEFR) and arterial blood gases were measured every 6?h postoperatively for 48?h.Results
The VAS, rescue doses of ketorolc and PEFR were significantly lower in the phrenic nerve group (P-value?<0.05). There was no statistically significant difference between the three groups postoperatively as regards arterial blood gases (P-value?>0.05).Conclusion
PNI is more effective than SNB for ISP. 相似文献30.
目的:探讨脱细胞关节软骨支架材料的制备方法,制备软骨理想的组织工程支架材料。方法:实验于2005-12/2006-08在兰州大学第二医院骨科研究所实验室完成。实验方法:利用冷冻干燥、化学去污剂等方法制备脱细胞的兔关节软骨。在无菌状态下取青紫蓝兔的新鲜兔关节软骨,剪成3.5mm×3.5mm,厚度0.2~2.0mm,在冷冻干燥器中冻干12h。在10g/L Triton X-100、Tris-HCl液内加入蛋白酶抑制剂-苯甲基黄酰氟,持续振荡48h后标本以双蒸馏水连续冲洗后置于DNase I酶和RNase A酶混合液中消化。置于10g/L Triton X-100、Tris-HCl液中洗脱。实验评估:①大体观察:肉眼观察脱细胞后关节软骨的外观形态。②组织学观察:将制备的脱细胞关节软骨行石蜡包埋切片,行苏木精-伊红染色、Massion三色染色并在光镜下观察。③扫描电镜观察:将制备的脱细胞关节软骨以戊二醛-锇酸双固定后,行扫描电镜观察。结果:①脱细胞后关节软骨外观形态:肉眼下可见正常关节软骨呈白色或淡黄色,脱细胞后关节软骨色呈灰白,半透明状,无光泽,外形与软骨相似并且仍维持了关节软骨的结构。②脱细胞后关节软骨的组织学变化:苏木精-伊红染色显示,软骨细胞消失,软骨巢分辨不清,在巢内没有蓝染的核物质,核细胞碎屑,只有红染为残留的细胞外基质。Massion三色染色显示,脱细胞后关节软骨内主要含有胶原纤维。③脱细胞后关节软骨的超微结构:扫描电镜下显示,脱细胞后关节软骨陷窝呈蜂窝状,未见到残余的细胞核、细胞器,残余的空穴高低不平。结论:经冷冻干燥、化学去污剂等方法可完整去除软骨中的细胞成分,保留胶原纤维等细胞外基质。 相似文献