Effects of synthetic muramyl dipeptide on bone metabolism in the fetal rat

Synthetic N-acetyl-muramyl-L-alanyl-D-isoglutamine (MDP) stimulated the release of 45Ca from fetal rat long bones in culture for 48 h and also increased the number of osteoclasts per bone and the number of nuclei per osteoclast. It also increased calcium uptake in enriched populations of osteoclasts obtained by sequential collagenase digestions of newborn rat calvaria. This effect was observed after a 120 min incubation with 10(-6) M MDP. Effects of MDP on calcium uptake in osteoblasts were not consistently observed. No effects on cyclic AMP were noted in either osteoclast or osteoblast populations treated with MDP for periods from 1-120 min at 37 degrees C.     

The role of intracellular lysosomal enzymes in the autocellular-surveillance of unhydroxylated collagens in dermal and gingival fibroblasts

The turnover of unhydroxylated collagen was investigated in dermal and gingival fibroblasts derived from C57Bl/6J mice. Unhydroxylated collagen molecules were fostered by the inhibition of prolyl- and lysyl-hydroxylase by the addition of alpha, alpha' dipyridyl. Turnover of collagens was determined by single isotope continuous labeling, double-isotopic labeling, the use of lysosomal pH modulators, and proteinase inhibitors. These studies reveal that the turnover of unhydroxylated collagen is an extracellular event, in spite of the susceptibility of these abnormal structural and conformational proteins to proteolysis; the synthesizing cell does not utilize intracellular lysosomal enzymes as a means of modulating the quantities of non-helical unhydroxylated collagen as an intolerable post-translational error of protein processing.     

In vitro fluoride uptake, distribution and retention by human enamel after 1- and 24-hour application of various topical fluoride agents

The in vitro fluoride acquisition by human enamel after a 1-hour and 24-hour application of APF, Duraphat (a resin varnish) or Fluor Protector (a polyurethane varnish) and subjection to various procedures was determined. Fluoride acquisition was the greatest in teeth treated with Fluor Protector and the least in APF-treated teeth. Fluoride uptake and distribution were increased by prolonging the contact time between the varnishes and enamel, and fluoride retention was decreased after subsequent exposure to synthetic saliva.     

A nonextraction approach to crowded dentitions in young children: early recognition and treatment

A total of 22 cases that were diagnosed as having space discrepancy problems in the deciduous and early mixed dentitions showed that treatment by early expansion therapy with Crozat appliances had eliminated the need for extraction of deciduous canines or permanent premolars.     

Dental regeneration and materials—a partnership

Considerable focus on the biocompatibility of dental materials over the last three decades has provided a platform for a wealth of studies on the cellular and molecular responses of the cells of the pulp to injury, both from the disease process and from subsequent restorative intervention. These studies have been fundamental to understanding not only how we can achieve a biocompatible response during restoration of dental disease but also how we can exploit the pulpal cellular responses to achieve wound healing and tissue regeneration in the dentine–pulp complex. This article examines the responses of the pulp to injury and the events leading to tissue regeneration. As new biologically based regenerative therapies emerge for the dental tissues, it is important that these develop in partnership with more traditional approaches using dental materials.     

Evaluation of the clinical behavior of resin modified glass ionomer cement on primary molars: a comparative one-year study

AIM: The objectives of the present study were to evaluate and compare the clinical behavior of resin modified glass ionomer cement (RMGIC) on primary molars with conventional and modified cavity preparations. METHODS AND MATERIALS: Forty-two children, 5-9 years of age, having bilateral initial occlusal caries on the mandibular primary second molars were selected for the study. A split mouth design was employed where conventional Class I cavities with a 90 degrees cavosurface angle were prepared randomly on primary second molars on one side and modified cavities with a 1 mm straight bevel along the cavosurface margin on the contra-lateral side. These cavities were restored with RMGIC. The restorations were evaluated during subsequent visits, for a period of one year. RESULTS: At the end of one year, 90% of the restorations survived in the conventional cavity group whereas 100% of the restorations survived in the modified cavity group. CONCLUSION: Incorporation of a bevel in Class I cavities increases the survival rate of RMGIC restorations. There was no significant difference in the clinical behavior between the two groups. However, beveling does contribute to long term clinical success of these restorations. CLINICAL SIGNIFICANCE: Incorporation of a straight bevel in conventional cavities will improve the retention of RMGIC by increasing the bonding area and enhancing the desired properties of the material.