99Tcm-神经紧张肽结肠癌显像的实验研究

目的　探讨99Tcm 神经紧张肽 (NT)显像早期诊断结肠癌的价值。方法　用99Tcm 标记NT进行裸鼠结肠癌模型体内生物分布和显像研究 ,观察受体阻断后99Tcm NT在裸鼠肿瘤模型体内的组织分布和显像特点。测定99Tcm NT与结肠癌细胞的亲和力常数 (Kd)。结果　99Tcm NT对结肠癌细胞Kd为 0 91nmol/L ;标记率 (>94 % )高且稳定。体内生物分布 :99Tcm NT主要经肾脏排泄 ,3和 12h肿瘤 /肌肉比值分别为 3 2 5± 1 0 2和 4 15± 1 4 6 ;受体阻断后 3h肿瘤 /肌肉比值 (1 2 1± 0 6 2 )明显降低 ,与受体未阻断结果相比差异有显著性 (P <0 0 1)。裸鼠肿瘤模型显像时间早 (0 5h开始显影 ) ,3h肿瘤 /对侧肢体比值为 2 6 8± 0 4 4 ;受体阻断后 3h肿瘤 /对侧肢体比值为 1 14± 0 36 ,与受体未阻断结果相比差异有显著性 (P <0 0 1)。结论　99Tcm NT制备方法简单 ,为结肠癌的早期特异性诊断提供了一种可能的新方法。     

IgA肾病肾动静态联合显像与肾脏病理学改变的关系

目的 分析肾动静态联合显像与IgA肾病（IgAN）病理学改变的关系.方法 选取肾脏组织病理检查证实为IgAN的患者20例,对其肾小球、肾小管间质和血管损害程度进行Katafuchi积分和Lee分级.患者均接受^99Tc^m-DTPA肾动态显像,其中13例另行^99Tc^m-二巯基丁二酸（DMSA）肾静态显像.图像经处理后获得灌注指数（PI）、肾小球滤过率（GFR）和肾/本底摄取比值（K/B）等参数.分别选择10例和4例健康体格检查者作为肾动静态显像正常对照.分析显像指标与病理积分间的关系,并比较各Lee分级的PI、GFR及K/B比值.结果 PI与血管损害积分呈正相关（左r=0.640、右r=0.725;P均＜0.01）,GFR、K/B比值与肾小球损害（GFR：总r=-0.731、左r=-0.729、右r=-0.718,K/B比值：左r=-0.714、右r=-0.686,P均＜0.01）及肾小管间质损害积分（GFR：总r=-0.619、左r=-0.628、右r=-0.595,K/B：左r=-0.712、右r=-0.716;P均＜0.05）呈负相关.随病理分级增高,PI均值无明显变化（左F=0.40、右F=0.44,P均＞0.05）,而GFR和K/B比值均值逐级降低（GFR：总F=52.23、左F=48.59、右F=29.66,K/B比值：左F=34.54、右F=23.06;P均＜0.01）.结论 肾动静态联合显像结果与肾脏组织病理学改变明显相关,其对IgAN病情评价及预后判断具有实用价值.     

核素显像对实验性绞窄性肠梗阻的早期诊断

用家兔制成闭袢型、肠系膜静脉阻塞型及肠系膜动脉阻塞型的急性绞窄性肠梗阻模型，并以单纯性肠梗阻组和假手术组为对照。制模后１、４小时从兔耳静脉注射显像剂９９ｍ锝焦磷酸盐，用单光子发射计算机断层（ＳＰＥＣＴ）进行腹部平面显像的动态观察，并测定感兴趣区的摄取比值。结果显示：在闭袢型和肠系膜静脉阻塞型于注射显像剂后，绞窄肠段所在腹侧出现放射性浓聚，显像部位感兴趣区摄取比值明显大于单纯性肠梗阻组和假手术对照组；肠系膜动脉阻塞型上述改变不明显。本实验结果提示：闭袢型和静脉阻塞型绞窄缺血肠段早期有显像剂的聚集；ＳＰＥＣＴ的核素显像技术可作为早期诊断闭袢型和肠系膜静脉阻塞型肠梗阻有价值的辅助手段。     

Prognostic value of postoperative stimulated thyroglobulin levels on 131Ⅰ ablation therapy in papillary thyroid cancer

Objective Stimulated thyroglobulin (Tg) levels postablation was associated with disease recurrence in papillary thyroid cancer (PTC). The aim of this study was to evaluate the prognostic value of postoperative stimulated Tg level on future Tg positivity after 131Ⅰ ablation therapy in PTC. Methods One hundred and thirty-eight patients (28 men, 110 women; age range 6-70 years, mean age 39.4 years) with PTC were included in this study. All patients underwent total or near-total thyroidectomy, and 102 of these patients had lymphadenectomy. All patients had a documented PTC. 131Ⅰ ablation was performed in 3- 4 weeks after thyroidectomy. Sera levels of thyroid hormones (FT3, FT4), thyrotropin (TSH), anti-Tg anti-body (TgAb), and Tg were measured before and after 131Ⅰ ablation. Statistical analysis was performed with SPSS 13.0 software, and correlation anaysis and t-test were used. Results Postoperative stimulated Tg lev-el had a significantly positive association with pestablation stimulated Tg level (r = 0. 960, P < 0.01). Postoperative stimulated Tg level in positive postablation Tg group was significantly higher than that in nega-tive pestablation Tg group [(199.8±327.7) μg/L vs (3.5±5.6) μg/L, t =5. 567, P <0.01]. About 76% (78/102) patients had evidence of metastatic cervical lymph nodes on routine histological testing. And 41% (446/1088) resected lymph nodes were histologically positive for metastatic disease. The number of metastatic lymph nodes resected had a significantly positive relationship with stimulated Tg at pestopera-tion and postablation (r = 0. 697, 0. 633, both P < 0.01). Conclusions Postoperative stimulated Tg level was of better prognostic value on stimulated Tg level after 131Ⅰ ablation therapy. Total or near-total thyroidec-tomy simultaneously conjugated with lymphadenectomy might have a better result in lower postablation stimu-lated Tg pesitivity in patients with PTC.     

Prognostic value of postoperative stimulated thyroglobulin levels on 131Ⅰ ablation therapy in papillary thyroid cancer

Objective Stimulated thyroglobulin (Tg) levels postablation was associated with disease recurrence in papillary thyroid cancer (PTC). The aim of this study was to evaluate the prognostic value of postoperative stimulated Tg level on future Tg positivity after 131Ⅰ ablation therapy in PTC. Methods One hundred and thirty-eight patients (28 men, 110 women; age range 6-70 years, mean age 39.4 years) with PTC were included in this study. All patients underwent total or near-total thyroidectomy, and 102 of these patients had lymphadenectomy. All patients had a documented PTC. 131Ⅰ ablation was performed in 3- 4 weeks after thyroidectomy. Sera levels of thyroid hormones (FT3, FT4), thyrotropin (TSH), anti-Tg anti-body (TgAb), and Tg were measured before and after 131Ⅰ ablation. Statistical analysis was performed with SPSS 13.0 software, and correlation anaysis and t-test were used. Results Postoperative stimulated Tg lev-el had a significantly positive association with pestablation stimulated Tg level (r = 0. 960, P < 0.01). Postoperative stimulated Tg level in positive postablation Tg group was significantly higher than that in nega-tive pestablation Tg group [(199.8±327.7) μg/L vs (3.5±5.6) μg/L, t =5. 567, P <0.01]. About 76% (78/102) patients had evidence of metastatic cervical lymph nodes on routine histological testing. And 41% (446/1088) resected lymph nodes were histologically positive for metastatic disease. The number of metastatic lymph nodes resected had a significantly positive relationship with stimulated Tg at pestopera-tion and postablation (r = 0. 697, 0. 633, both P < 0.01). Conclusions Postoperative stimulated Tg level was of better prognostic value on stimulated Tg level after 131Ⅰ ablation therapy. Total or near-total thyroidec-tomy simultaneously conjugated with lymphadenectomy might have a better result in lower postablation stimu-lated Tg pesitivity in patients with PTC.     

双核素试餐对非溃疡性消化不良患者胃肠运动功能的研究

双核素试餐对非溃疡性消化不良患者胃肠运动功能的研究高再荣张永学詹浩熊洁胡佳侯晓华一般认为，非溃疡性消化不良（ＮＵＤ）与胃运动功能障碍有关〔１〕。我们对５２例ＮＵＤ病人的胃排空功能和小肠通过时间进行了观察。资料与方法一、研究对象对照组１８例，男１０例、...     

Survivin反义寡核苷酸的99mTc标记及在肝癌细胞中的表达

目的 :研究放射性核素99mTc标记Survivin反义寡核苷酸 (ASON)的方法及在肝癌细胞中的动力学 ,探讨用于肝癌诊断的可能性。方法 :用DNA合成仪合成 18碱基单链SurvivinASON ,在 5′末端经氨基修饰后 ,用S acetyl NHS MAG3作螯合剂 ,进行99mTc标记SurvivinASON ,并对其标记率、放化纯、比活度、稳定性、细胞摄取率和细胞清除率进行了研究。结果 :99mTc MAG3 ASON的标记率为 65 .2 2 %,放化纯为 94%,比活度为 1190kBq/μg。室温条件下与生理盐水温育 4h后 ,其放化纯为 92 %。 3 7℃ 5 %CO2 条件下与新鲜人血清温育 4h ,未见明显的血清蛋白结合作用。肝癌细胞摄取率 10h达 12 .73 %,细胞滞留率 8h为 5 4.2 2 %。结论 :采用S acetyl NHS MAG3 作螯合剂标记Survivin ASON的方法可行 ,可获得较好的标记效果 ,99mTc survivin ASON在肝癌细胞中有较理想的摄取率 ,可进一步研究用于肿瘤基因显像诊断的可能性