术前应用氨溴索对左心室舒张功能不全老年患者开胸术后疗效研究

Objective: To investigate curative effect of preoperative use of ambroxol in elderly patients with left ventricular diastolic dysfunction after thoracic surgeries. Methods: Eighty eight cases of elderly patients in hospital were collected,who all had left ventricular diastolic dysfunction, but whose six minutes walking distance>300 m. The patients were divided into experimental group and control group, ambroxol were used pre-operation, while placebo were used in the control group. Static pulmonary function, Doppler ultrasound, C reactive protein (CRP), brain natriuretic peptide(BNP), procalcitonin(PCT) were detected 3 days before and after the operation ,8 days after operation. Vital signs and complications of heart and lung post operation were also monitored and analyzed. Results: The lung function and heart function in the experimental group were better than those in the control group, (P<0.05).The vital signs and arterial blood gas of the patients in the experimental group after the thoracic surgeries were more stable than those in the control group, P<0.05.Laboratorial index includes BNP,CRP ,PCT of the experimental group was lower than that in the control group, (P<0.05).The incidence of pulmonary infection ,pulmonary atelectasis, heart failure, arrhythmia in the experimental group after the thoracic surgeries were lower than that in the control group, （P<0.05）.Conclusion: Preoperative use of ambroxol can reduce the surgical trauma, improve heart and lung function in patients after the thoracic surgeries, as well as decrease the incidence of lung and heart complications. It also can help patients better adapt to the operation, leading to lower adverse effect.      

梅花桩手穴按压对胸腰椎骨折后便秘患者的干预研究

目的观察梅花桩手穴按压对胸腰椎骨折后便秘患者的临床应用效果。方法按随机数字表将84例胸腰椎骨折后便秘患者分为观察组和对照组各42例,两组均予常规护理措施干预,观察组加用梅花桩手穴按压,观察两组患者排便、腹痛、腹泻情况。结果观察组有效率88.1%,对照组有效率42.9%,两组比较,x2=20.84,P0.05。两组患者均无腹痛、腹泻,观察组手穴按压处皮肤无破损和糜烂。结论梅花桩手穴按压治疗胸腰椎骨折后便秘效果较好。     

不同碘摄入量对子代大鼠脑神经递质及其相关酶活性的研究

目的通过研究子代大鼠脑组织中神经递质含量及相关酶活性,探讨不同碘摄入量对脑功能的影响。方法断乳后1月的W istar大鼠随机分为4组:低碘组,适碘组,10倍碘组,50倍碘组,分别饮用含不同碘浓度的水,饲养3月后随机交配,仔鼠饲养28 d后,断头取其大脑组织,称重,测定单胺氧化酶(MAO)和胆碱酯酶(ChE)活性。结果与适碘组相比,其它各组大鼠脑重量均明显降低(P<0.05或P<0.01),但低碘组最明显;20%脑组织匀浆中蛋白质含量,低碘组明显降低(P<0.01);低碘组仔鼠脑内MAO活性明显升高(P<0.01),ChE活性明显降低(P<0.01);50倍碘组大鼠MAO活性明显升高(P<0.01)。结论碘缺乏和严重碘过量可影响子代大鼠脑的神经递质及其相关酶活性,但碘缺乏的影响远比碘过量严重。     

靶向c-Met嵌合抗原受体T细胞的制备及其对鼻咽癌细胞作用的研究

目的 构建靶向c-Met的嵌合抗原受体(CAR)逆转录病毒载体,制备靶向c-Met CAR-T,观察其对c-Met阳性鼻咽癌细胞的杀伤作用。 方法 利用基因工程技术构建抗c-Met scFv,并将其重组到含有CD28,CD137,CD3ζ等的逆转录病毒载体中,形成c-Met CAR逆转录病毒载体,测序确认。以该病毒载体感染T淋巴细胞,通过Western blotting检测c-MetCAR在T淋巴细胞中的表达。CCK-8检测c-Met CAR-T对鼻咽癌细胞的作用;通过ELISA检测c-Met CAR-T作用后IFN-γ、IL-2的变化。 结果 测序结果显示c-Met CAR病毒载体序列正确;Western blotting可检测到CD3ζ的表达;CCK-8结果显示,c-Met CAR-T明显抑制c-Met阳性的鼻咽癌细胞的增殖(P<0.05);ELISA结果显示,c-Met CAR-T作用后分泌IFN-γ、IL-2明显增加(P<0.01)。 结论 成功制备了靶向c-Met的嵌合抗原受体逆转录病毒载体。该嵌合抗原受体能在T细胞中表达,能有效抑制c-Met阳性鼻咽癌细胞增殖,增加IFN-γ、IL-2的分泌。     

手指淋巴管的应用解剖

目的　研究人体指淋巴管的解剖特征为临床提供解剖学基础。 方法　2具新鲜成人尸体4例手标本,指尖两侧皮内,注入少量双氧水墨水混合剂。于真皮下找到淋巴管,将显影剂经30G注射针注入。追踪指淋巴管的行程并进行照像和X线记录。1例标本作指截面研究。 结果　各指两侧皮下各分布淋巴管1支,近指根处偶见多支。它们始于远侧指间关节两侧真皮下、沿指中轴两侧皮下组织蜿蜒起伏地行走。管径0.2~0.8 mm,近端较粗,远端较细。除拇指桡侧和小指尺侧淋巴管各自在掌背尺、桡侧汇入手背淋巴管外,其他指淋巴管在指蹼处与邻近淋巴管吻合,再汇入掌背淋巴管。指横截面显示指淋巴管与周围组织的解剖关系。 结论　详细描述了各指淋巴管的解剖形态,以及与周围组织、血管神经的关系,为临床和科研提供解剖学基础。