针灸治疗神经病理性疼痛的实验研究进展

从针灸对阿片肽、五羟色胺、神经胶质细胞、细胞因子、氨基酸及嘌呤类物质的影响入手探讨针灸治疗神经病理性疼痛(NP)的机制,指出针灸作为中医学特色疗法之一,在治疗神经病理性疼痛的临床疗效和安全性已被认可,然而其相关机制仍旧未完全阐明,可能与针灸能激活自身机体功能,对上述物质进行多层次、多靶点的调节有关。     

壳聚糖预防眼外肌手术粘连的实验研究

目的 了解壳聚糖预防术后眼外肌粘连的效果,为临床应用提供实验依据.设计自身对照实验研究.研究对象家兔15只（30眼）.方法 采用家兔自身对照方法,右眼为实验组,左眼为对照组,分别制作上直肌周围眼球筋膜缺损模型,缺损区实验组用2%壳聚糖凝胶,对照组用平衡盐溶液.主要指标术后4周对术区行大体观察及组织学光镜观察成纤维细胞数目及形态.结果 术后4周,大体观察实验组术区组织粘连程度较对照组明显为轻,组织学光镜观察实验组结膜与眼肌组织之间粘连较轻,瘢痕组织增生较少,成纤维细胞数目较少,体积小,排列整齐,较疏松,成熟细胞较少;对照组结膜与眼肌组织粘连重,瘢痕组织增生多,大量成纤维细胞,排列紊乱、密集,多数呈梭形,趋于成熟.结论 壳聚糖能减轻眼外肌术后组织粘连,无明显不良反应.     

麦粒灸配合三棱针挑刺治疗扁平疣的疗效观察

目的观察麦粒灸配合三棱针挑刺治疗扁平疣的临床疗效。方法将104例扁平疣患者随机分为治疗组53例与对照组51例。治疗组采用麦粒灸配合三棱针挑刺治疗,对照组采用外涂维A酸乳膏治疗。比较两组近期(治疗后)和远期(治疗后3个月)的疗效。结果治疗组治疗后总有效率和痊愈率分别为90.2%和62.7%,对照组分别为86.0%和48.0%,两组比较差异具有统计学意义(P0.05)。治疗组治疗后3个月总有效率和痊愈率分别为92.2%和64.7%,对照组分别为86.0%和48.0%,两组比较差异具有统计学意义(P0.05)。结论麦粒灸配合三棱针挑刺是一种治疗扁平疣的有效方法。     

LASIK后弥漫性板层角膜炎的临床分析

Objective To study the causes,clinical features and prevention measures of diffuse lamel-lar keratitis (DLK)after LASIK. Methods Eleven patients ( 17 eyes) with DLK after LASIK among 199 pa-floats (398 eyes) from March 2007 to December 2007 were selected as observation group,and after the strict checks on operating room environment,pressure sterilizer,surgical instrument disinfection,preoperative con-junctival sac clean and corneal epithelium protection,5 patients (7 eyes) with DLK after LASIK among 148 patients (296 eyes) from December 2007 to May 2008 were selected as control group. Each stage's complex,clinical features and treatment process of DLK were analyzed. Results The incidence rate of DLK in obser-vation group was 4.27%,among which,stage Ⅰ was 3.01%,stage Ⅱ was 0.5% and stage Ⅳ (3 eyes) was 0.75%. The incidence rate of DLK in control group was 2.36%,among which,stage Ⅰ was 1.68% and stage Ⅱ was 0.67%. DLK of stage Ⅰ and stage Ⅱ happened 1～3 days after LASIK,it absorbed entirely after 7～10 days'treatment, and vision rocovered to normal. DLK of stage Ⅳ happened 3～7 days after LASIK, relatively white dense opacity under the lamellar of central cornea and flap melt could be seen through slit lamp. High hyperme-tropia was left after healing. Hypermetropia decreased partly after 3～6 months, and vision recovered to 0.6～1.0. Conclusion The occurrence of DLK after LASIK is related with multifactors. DLK can be prevented by the strict checks on each link of the operation and cured better if it is treated in time.     

LASIK后弥漫性板层角膜炎的临床分析

Objective To study the causes,clinical features and prevention measures of diffuse lamel-lar keratitis (DLK)after LASIK. Methods Eleven patients ( 17 eyes) with DLK after LASIK among 199 pa-floats (398 eyes) from March 2007 to December 2007 were selected as observation group,and after the strict checks on operating room environment,pressure sterilizer,surgical instrument disinfection,preoperative con-junctival sac clean and corneal epithelium protection,5 patients (7 eyes) with DLK after LASIK among 148 patients (296 eyes) from December 2007 to May 2008 were selected as control group. Each stage's complex,clinical features and treatment process of DLK were analyzed. Results The incidence rate of DLK in obser-vation group was 4.27%,among which,stage Ⅰ was 3.01%,stage Ⅱ was 0.5% and stage Ⅳ (3 eyes) was 0.75%. The incidence rate of DLK in control group was 2.36%,among which,stage Ⅰ was 1.68% and stage Ⅱ was 0.67%. DLK of stage Ⅰ and stage Ⅱ happened 1～3 days after LASIK,it absorbed entirely after 7～10 days'treatment, and vision rocovered to normal. DLK of stage Ⅳ happened 3～7 days after LASIK, relatively white dense opacity under the lamellar of central cornea and flap melt could be seen through slit lamp. High hyperme-tropia was left after healing. Hypermetropia decreased partly after 3～6 months, and vision recovered to 0.6～1.0. Conclusion The occurrence of DLK after LASIK is related with multifactors. DLK can be prevented by the strict checks on each link of the operation and cured better if it is treated in time.     

大肠杆菌在巨噬细胞中的降解与交叉递呈研究

目的 通过表达的红色荧光蛋白(DsRed)与卯白蛋白(ovalbumin,OVA)表位融合蛋白,研究表达此融合蛋白的大肠杆菌在巨噬细胞中降解与交叉递呈的时相.方法 构建红色荧光蛋白与卵白蛋白表位融合蛋白的原核表达质粒(pET-16bOR),流式细胞计数仪检测表达融合蛋白的大肠杆菌,然后动态观察此大肠杆菌在巨噬细胞中的降解与交叉递呈.结果 表达红色荧光蛋白(DsRed)与卵白蛋白(ovalbumin,OVA)表位融合蛋白的大肠杆菌能被488 nm波长激发荧光.在巨噬细胞中,内质网与吞噬的大肠杆菌共聚;吞噬的大肠杆菌3～5 h快速降解;而在此时相产生的H-2Kb-OVA257-264复合物达到顶峰.结论 表达此红荧光蛋白与OVA表位融合蛋白的大肠杆菌具有荧光特性,内质网参与大肠杆菌吞噬体形成,巨噬细胞吞噬大肠杆菌后3～5 h是抗原快速降解和交叉递呈的时相.     

机体微环境失调在原发性胆汁性胆管炎发病机制中的作用

原发性胆汁性胆管炎（PBC）是一种慢性进展性自身免疫病,其主要病理表现为肝内胆汁淤积,胆管上皮细胞炎症损伤及肝内中、小胆管的持续性炎症损伤,最终导致肝细胞发生不可逆损伤。PBC的发病机制包括遗传、汇管区炎症损伤、黏膜免疫失调和胆管上皮细胞功能改变,肝脏和机体整体的微环境失调是诱发肝纤维化和肝硬化的始动因素。该文就机体代谢微环境、炎症反应微环境和免疫微环境失调在PBC发病机制中作用的研究进展作一综述。     

壳聚糖膜在眼表碱烧伤早期应用的实验研究

 目的 了解壳聚糖膜用于早期眼表碱烧伤预防睑球粘连的效果,为临床应用提供依据.方法 家兔15只,采用自身对照方法,右眼为实验组,左眼为对照组,分别制作眼表碱烧伤模型,实验组于结膜囊中放置壳聚糖膜,对照组不用.术后8周对术区行大体观察及组织学光镜观察.结果 实验组及对照组均发生严重睑球粘连,在组织学上未见明显差异,实验组15例标本均发生角膜穿孔,眼内容物脱出,对照组角膜呈白色混浊,外有纤维组织包裹,未见角膜溃疡、穿孔.结论 对于严重眼表化学烧伤,不能早期应用抗粘连药物,以免发生角膜穿孔,待角膜稳定后才可以应用壳聚糖膜.     

西地碘含片口腔黏膜刺激性试验

目的：观察西地碘含片对金黄地鼠口腔黏膜的刺激性。方法：将金黄地鼠随机分为4组,分别为西地碘含片完整黏膜组,西地碘含片破损黏膜组,赋形剂完整黏膜组,赋形剂破损黏膜组,每组6只,经口腔黏膜给药7d后作大体观察评价和组织学评价。结果：大体观察显示西地碘含片对金黄地鼠的口腔完整黏膜和破损黏膜均无刺激性;病理组织学检查显示西地碘含片对完整黏膜和破损黏膜均不产生炎症等病理学改变;病理组织学评分显示西地碘含片对完整黏膜和破损黏膜均无刺激性。结论：西地碘含片对金黄地鼠的口腔黏膜无刺激性。     

复方熊胆眼用即型凝胶局部眼刺激性试验

目的：观察复方熊胆眼用即型凝胶对家兔眼局部用药的刺激性。方法：将家兔随机分为复方熊胆眼用即型凝胶和赋形剂2个组,采用左右自身对照进行眼刺激试验,在给药前、给药期间及停药后按时间点用裂隙灯显微镜对家兔双眼角膜、虹膜、结膜进行观察,对损伤程度按评分标准进行评分。结果：复方熊胆眼用即型凝胶滴眼液对家兔眼局部刺激平均反应积分值为0。结论：复方熊胆眼用即型凝胶滴眼液对家兔眼局部无刺激性。