J2对同种异体穿透性角膜移植模型大鼠CD4+和CD8+T细胞免疫功能的抑制

BACKGROUND: J2 takes functional domain (MHC CD4-D1/) of complex conjugate of CD4 molecule and MHC class II molecule as a target, and is a small molecule compound obtained by computer screening from a chemical data containing hundreds of thousands of organic compounds. In the previous study, J2 was used in mouse models of skin transplantation and keratoplasty by oral and intraperitoneal injection. Results verified that J2 could prolong the survival time of grafts, and suppress occurrence of rejection. To better play the role of a drug targeting and to reduce systemic toxicity, J2 will be further utilized in local treatment of keratoplasty rejection. OBJECTIVE: To investigate the inhibitory effect of new immunosuppressive agent J2 on CD4+ and CD8+ T cell immune functions in rat models receiving allogenic penetrating keratoplasty. METHODS: Allogeneic penetrating keratoplasty model was established using the adult female Wistar rats as donors and Sprague-Dawley rats as recipients. Group A: normal Sprague-Dawley rats were injected with 0.05 mL placebo subconjunctivally. Surgery rats were randomly divided into three groups. Group B: allograft rats were injected with 0.05 mL placebo subconjunctivally after autologous keratoplasty. Group C: allograft rats were injected with 0.05 mL placebo subconjunctivally. Group D: allograft rats were injected with 1% J2-nanosuspension 0.05 mL subconjunctivally. The distribution of T cell subsets in peripheral blood was detected using flow cytometry at 3 days, 1, 2 and 3 weeks after transplantation and compared among groups.  RESULTS AND CONCLUSION: There was no significant difference in total CD3+ T cells, CD4+ T cells, CD8+ T cells and CD4+/CD8+ in peripheral blood lymphocytes in group B at various time points. At 3 days and 1 week after surgery in group C, no significant difference in total CD3+ T cells, CD4+ T cells and CD8+ T cells was detected. At 1 and 2 weeks, the number of total CD3+ T cells, CD4+ T cells and CD8+ T cells increased, showing significant differences (P < 0.05). In group D, no significant hyperplasy was found in CD4+ T cells and CD8+ T cells at 1 and 2 weeks. The horizontal comparison of the same time point: the total CD3+ T lymphocytes of group D was significantly less than group C at 3 days, 1 and 2 weeks after operation (P < 0.05), whereas there was no significant difference at 3 weeks between the group D and group C. The number of CD4+ T lymphocytes in group D was less than in group C at 3 days and 1 week, but with no significant difference. The ratio of CD4+/CD8+ had no significant difference in group D compared with group C at 3 days, 1 and 3 weeks. J2 inhibits T lymphocyte proliferation and then inhibits T cell-mediated corneal allograft rejection.       

烫伤灵对小鼠抗炎、镇痛作用的实验研究

目的研究烫伤灵的药理作用。方法采用巴豆油致炎模型，用烫伤灵高、低剂量皮下注射。30min后处死取小鼠腹腔液测定其对毛细血管通透性的影响；同时采用扭体法、热板法，考察镇痛作用。结景烫伤灵高剂量组与獾油组均有较强的抗炎作用（P〈0．01）；烫伤灵与空白组比较均有明显的镇痛作用（P〈0．01）。结论烫伤灵对动物体表深度烫伤有较好的治疗作用，具有明显的镇痛、抗炎作用。     

癌消Ⅰ号对肝癌H22和艾氏腹水瘤小鼠的影响

目的研究癌消Ⅰ号对肝癌22和艾氏腹水瘤小鼠的作用。方法以肝癌（H22）小鼠和艾氏腹水瘤（EAC）为模型，观察癌消Ⅰ号的抗肿瘤作用，并分别计算出抑瘤率和生命延长率。结果相当于生药3、6、12g／kg的癌消Ⅰ号对肝癌（H22）小鼠的抑瘤率均在40％以上，与对照组比较差异均有显著性意义，P〈0．01；能延长艾氏腹水瘤（EAC）小鼠的生．存期，生命延长率均在50％以上，与对照组比较差异均有显著性意义，P〈0．01。结论癌消Ⅰ号对肝癌H22和艾氏腹水瘤有明显作用。     

蠲痹抗生丸对寒凝血瘀型大鼠血液流变学的影响

目的 研究蠲痹抗生丸对大鼠血液流变性的影响.方法 复制大鼠寒凝瘀血模型,检测蠲痹抗生丸对寒凝瘀血大鼠血液流变性指标的影响.结果 蠲痹抗生丸中高剂量组能显著降低寒凝瘀血模型的全血高切和低切黏度,全血高切还原黏度.结论 蠲痹抗生丸具有改善血流变性的作用,这可能是其活血化瘀治疗关节炎的中药药理学基础之一.     

室速积分法对预激性心动过速的鉴别诊断意义

目的:探讨室性心动过速(室速)积分法对预激性心动过速的鉴别诊断意义。方法:选取2010-01-2018-11收集的30例经过心内电生理确诊的预激性心动过速患者发作时的宽QRS波心电图,用室速积分法对预激性心动过速心电图进行评估,分析积分法的单项指标诊断的特异度,计算总积分并分析不同积分诊断的特异度。结果:(1)室速积分法的7项指标特异性都达60%以上,但除V1导联起始为R,Ⅱ导联RWPT≥50ms,房室分离这3项指标有统计学意义外(P0.05),其余P值都大于0.05。其中房室分离特异度最高,达100%。(2)总积分得0分的为33.3%,积1分的为43.3%,积2分的为23.3%,≥3分的为0%,其特异度分别为66.7%、56.7%、76.7%、100%。结论:(1)积分法7项指标中以房室分离诊断预激性心动过速特异度最高,达100%。(2)室速积分法2分尚不能完全鉴别室速与预激性心动过速,但≥3分特异度为100%,可有效鉴别室速与预激性心动过速,应用室速积分法鉴别这两种心动过速具有较高的临床推广价值。     

127例患者PICC置管护理体会

目的:探讨PICC置管技术在临床中应用的实用性.方法:回顾性分析127例行PICC置管患者的临床资料(PICC组),并与同期行颈静脉置管的68例患者(CBP组)在置管成功率、并发症发生率、导管留置时间上相比较.结果:PICC置管技术在置管成功率上与颈静脉置管无差异,而在并发症发生率及导管留置时间上均优于经静脉置管.结论:PICC技术在临床上有较实用及广泛的应用前景.     

三种准分子激光角膜切削术后中央角膜厚度变化的对比研究

目的比较三种准分子激光角膜切削术后中央角膜厚度(CCT)的差异并探讨不同手术方式角膜切削量的设定。方法使用Pentacam三维眼前段分析仪测量行SBK、LASIK、Epi-LASIK术的近视患者共136例266只眼术前术后的CCT,按屈光度分为低、中、高三组,分别计算和比较CCT及实际切削深度和设计切削深度的差值(Diff值)。结果Epi-LASIK患者低、中、高度组术前CCT分别为(495.70±24.94)、(501.57±31.34)和(525.18±16.53)μm,较SBK和LASIK组患者CCT明显偏薄(P0.01),SBK与LASIK患者术前术后的CCT无显著性差异。SBK、LASIK术后Diff值比较:低度组Diff值分别为(6.57±12.72)、(5.33±9.51)μm;中度组Diff值分别为(7.07±18.19)、(4.42±8.72)μm;高度组分别为Diff值(11.52±10.29)、(7.00±7.33)μm,各组比较差异均无显著性意义。三种手术方式的实际切削的角膜深度均大于设计量。结论CCT较薄的患者多选择Epi-LASIK。充分估计术中实际切削深度与设计切削深度存在的差异是提高手术安全性的保障。     

158例子宫肌瘤及99例子宫内膜癌患者ST—T改变的对比分析

本文分析2000年1月～2000年12月158例子宫肌瘤住院病人及99例子宫内膜癌住院病人的心电图,以探索子宫肌瘤与子宫内膜癌心电图ST-T改变的关系。 1 资料与方法 选择158例子宫肌瘤病人(A组),     

流程再造在结肠癌术后化疗患者心理护理方面的应用

目的:探讨流程再造在结肠癌术后化疗患者心理护理方面的应用及护理体会。方法:通过成立流程再造小组,制定心理护理跟踪手册,根据本科室特点及科内骨干护士多年的临床工作经验,经多次会议及讨论,制定出了一套结肠癌术后化疗患者心理护理的流程再造实施方案。进行为期9个月对98例患者进行实施方案与跟踪。结果:患者的满意程度明显高于实施前,并在给药过程中,患者及其家属的配合程度和积极性也有了明显的改善。参与调查的98例化疗患者均安全满意的结束化疗。结论:通过方案的实施,提高了患者的满意度,巩固了患者的社会支持系统,提高化疗患者的生存质量,延长其生存期,建立和谐融洽的护患关系。     

降糖延生袋提取物对几种高血糖动物模型的作用研究

将降糖延生袋(地黄、山药、石斛、天花粉、元参、玉竹、黄芪等)水提醇沉液浓缩制成一定浓度溶液,对正常家兔空腹血糖和外源性注射肾上腺素引起的高血糖以及注射葡萄糖所致的小鼠高血糖均有降抑作用,并对四氧嘧啶破坏小鼠胰岛β细胞所致的高血糖也有良好作用。提示本方具有胰岛素样作用。