THE HIGH-LEVEL EXPRESSION OF nm23(NDP)GENE IN NPC

WE have studied the expression of nm23(NDP)in 50cases nasopharyngeal biopsies with anti-nm23(NDP)antibodies. As a result, the NDP positive rate innasopharyngeal carcinoma(NPC)(95.54%) markedlyincreased (P<0.05), as compared with that in the normalnasopharyngeal epithelia (50.00-60.00%) and lympho-cytes (52.00%). There were cytoplasmic type, nucleustype and mixed cytoplasmonucleus type according to NDP,location in a cell Their positive rates were 64.44%,15.56%, and 20.00%, respectively in nasopharyngealcarcinoma. The expression of NDP had no relation withcervical lymphometastases in NPC, and the NDP positiverates had no significance between bilateral cervicallymphometastases and unilateral(P<0.05). But the NDPexpression had most relation with the NPC staging. Theexpression rate and the intensity in Ⅲ or Ⅳ stagepatients were markedly higher than that in Ⅱ stage. Itpoints out that the high-level expression of NDP hadrelation with the rapid cellular proliferation in NPC, andit may indicate the bad prognos     

Establishment of K562/ADM/VER cell subline resistant to verapamil and its resistant mechanism

Failure of chemotherapy for cancer results mainly from resistance to chemotherapy. Tens kind of reversors have been found to reverse resistance of tumor cell to anticancer drugs in vitro. Interestingly, tumor cells were observed to develop resistance to the resistance reversors recently. Multidrug resistant cells, EMT16 have been reported to produce resistance to verapamil (VER), a resistance reversor, from Reeve JG, et al.[1]. However, this fact has not been fully paid attention with no r…     

田基黄提取物与5-FU联合应用治疗肝细胞癌的实验性研究

目的研究田基黄提取物对H22荷瘤小鼠的抗肿瘤作用及其对化疗药物氟尿嘧啶（5-FU）的增效减毒作用。方法采用荷H22肝细胞癌小鼠为模型,在体观察田基黄醇提液和水提液单独应用以及与5-FU联合应用对荷瘤小鼠抑瘤率、脾指数和胸腺指数等的影响。结果田基黄醇提液和水提液对H22移植瘤具有一定的抑制作用,抑瘤率分别为11.49%和21.41%,两者与5-FU联合应用能提高后者对H22移植瘤的抑瘤率,但差异均无统计学意义（P〉0.05）。与对照组相比,田基黄醇提取液和水提液也能显著升高H22荷肝细胞癌小鼠的脾指数和胸腺指数。结论田基黄提取物对肝细胞癌具有一定的抑制作用,对5-FU具有一定的减毒增效作用。     

nm23蛋白在人鼻咽癌中的表达

ｎｍ２３蛋白在人鼻咽癌中的表达郑天荣谢佐福林贤东张竟时陆莉莉最新研究资料表明，ｎｍ２３基因的高表达仅与部分肿瘤如肺癌、乳腺癌、肝癌等癌症的转移能力下降有关［１］，而与另一些肿瘤如结肠癌、甲状腺癌的转移无关［２］。鼻咽癌在我国南方发病率高，其淋巴结转移...     

P21和P53蛋白在人鼻咽癌中的表达

Ｐ２１和Ｐ５３蛋白在人鼻咽癌中的表达郑天荣谢佐福张竞时林贤东蔡晓文一、材料与方法选择我院１９９４年２月～１０月经病理证实的鼻咽活检组织５０例。其中低分化鳞状细胞癌４５例，泡状核癌和低分化腺癌各１例，鼻咽慢性炎症３例。组织经１０％福马林固定，石蜡包埋，...     

Regulation of expression of P-glycoprotein and gst by multidrug-resistant reversors in adm-sensitive and adm-resistant human tumor cell lines

Threemultidrugresisors,verapamil(VER),dipyriamole(DPM),andcyclosporina(CsA),havebeenfoundtoovercomemultidrugresistanceinvarioustumorcellsmainlythroughcompetivecombinationwithanticancerdrugsforp-glycoprotein(PgP)fromoursandotherinvestigatorsfindings..d.li-3]Wereportedpreviouslytheregulativeeffectofthesereversorsonsevenkindsofoncoproteinsortumorsuppressoroncoproteinsexpressions.fWereportheretheregulationofPgPandGSTexpressionfromthesereversorsinADM-sensitiveandADMresistanthumanleukemicce…     

宫颈癌组织端粒酶活性在诊断与预后中的意义

目的 探讨宫颈癌组织端粒酶活性在诊断预测患者预后方面的意义。方法 应用多聚酶链反应－酶联免疫吸附法（ＰＣＲ－ＥＬＩＳＡ）对病理确诊的３５例宫颈癌组织和１２例宫颈炎组织进行端粒酶活性半定量检测。结果 ３５例宫颈癌组织中３１例端粒酶阳性表达，端粒酶阳性率为８８．６％（３１／３５）。１２例宫颈炎组织中有２例端粒酶阳性表达。端粒酶Ａ值的高低与组织分化、临床期别有关，分化差、晚期癌Ａ值明显增高。结论 宫颈癌     

我校生理学计算机试题库建立初探

生理学作为研究正常机体生命活动规律的科学,是医学专业的基础课程,也是临床医学、基础医学及医学相关专业的核心课程。该学科是医学生完成正常人体形态学课程后首次接触的机能学科,为后续药理学、病理生理学等课程及临床各学科学习奠定基础,且可培养医学生分析和解决问题的能力,为其毕业后从事医学相关工作奠定基础。     

亚硝胺诱发大鼠食管癌survivin mRNA转录水平及与病理变化的关系

 目的探讨甲基苄基亚硝胺（MBNA）诱发食管癌变不同阶段食管组织survivin mRNA转录水平的变化及其与食管组织病理改变的关系。方法Wistar大鼠按3.5 mg/kg体重剂量皮下注射MBNA溶液,每周2次,分别于造模第10、20、30周各处死8只模型大鼠,以同批次正常大鼠为对照,观察食管黏膜大体情况,常规固定切片,并提取新鲜食管组织总RNA,RT-PCR检测survivin mRNA转录水平。结果MBNA诱导10、20及30周时,食管病变呈进行性加重;10、20及30周时survivin mRNA转录水平（0.48±0.16、0.42±0.15、0.46±0.17）差异无统计学意义（P>0.05),但均较正常大鼠（0.24±0.13）显著提高（P<0.01或P<0.05)。结论MBNA诱导大鼠食管癌变过程中,survivin mRNA转录水平在食管早期病变阶段即显著升高且在癌变过程中持续维持较高转录水平,因此抑制survivin mRNA转录是食管癌防治的一个潜在分子靶点。     

EFFECT OF ASCORBIC ACID ON DNA SYNTHESIS,INTRACELLULAR ACCUMULATION OF ADM AND ADM RESISTANCE OF TUMOR CELL LINES

ＥＦＦＥＣＴＯＦＡＳＣＯＲＢＩＣＡＣＩＤＯＮＤＮＡＳＹＮＴＨＥＳＩＳ，ＩＮＴＲＡＣＥＬＬＵＬＡＲＡＣＣＵＭＵＬＡＴＩＯＮＯＦＡＤＭＡＮＤＡＤＭＲＥＳＩＳＴＡＮＣＥＯＦＴＵＭＯＲＣＥＬＬＬＩＮＥＳＸｉｅＺｕｏｆｕ谢佐福ＬｉｎＸｉａｎｄｏｎｇ林贤东Ｚｈｏ...