八珍汤加味治疗头痛30例

头痛是临床常见的病症之一,笔者在临床中对辨证属气血亏虚型头痛者,以八珍汤加味治疗,每每获效,结果报道如下.     

重组牛胰蛋白酶抑制剂对大鼠急性胰腺炎的治疗作用

目的：观察重组牛胰蛋白酶抑制剂（rBPTI）对大鼠急性坏死性胰腺炎（ANP）的治疗作用。方法：取大鼠60只，随机分成6组：假手术组、模型组、rBPTI的3个剂量组、抑肽酶组。注射牛磺胆酸钠建立大鼠ANP模型，股静脉注射给药48h后，测定专鼠血清和组织淀粉酶和脂肪酶含量，观察胰腺组织病理变化。结果：rBPTI治疗组可明显降低血清和组织淀粉酶、脂肪酶的浓度，与模型组比较差异有统计学意义（P〈0．05，P〈0．01），且使胰腺的病理变化减轻。结论：rBPTI对牛磺胆酸钠诱导大鼠ANP具有治疗作用，作用效果同天然BPTI相当。     

中等职业学校英语分层教学探索

两千多年前，著名教育家孔子就提出了因材施教的教育思想，他承认学生在个性与才能上存在差异并主张根据学生的个性与特长有针对性地进行教育^[1]。分层教学便在因材施教思想下产生，并在课堂教学中体现。所谓的分层教学是指按学生实际学习程度，把学生分成不同层次进行教学的一种教学组织形式，它充分考虑到学生的个体差异，     

疗养飞行人员非运动外伤4例分析

20 0 0年以来 ,我科健康疗养飞行人员在日常生活娱乐中 ,发生 4人外伤 ,现将受伤治疗情况介绍如下 :一、临床资料例 1,男 ,40岁 ,飞行教员。喝酒后回疗养楼 ,因大门紧闭 ,玻璃透明 ,误以为没有玻璃门阻隔 ,径直向楼内闯去 ,迎面撞碎铝合金玻璃门 ,双眼眶周围、额部多处软组织挫伤 ,皮下瘀血、红肿 ,经冷敷、止痛及理疗后治愈。例 2 ,男 ,37岁 ,飞行员。星期天在疗养室内打扑克一整天 ,自觉双手发僵、发麻 ,请同伴拽拉活动 ,因动作过猛 ,感觉左上臂内侧象刀割样疼痛 ,左肱二头肌中段随即肿胀 ,屈肘时症状加重 ,临床考虑为左肱二头肌急性拉伤 …     

β受体激动剂对急性肺损伤大鼠肺表面活性物质含量的影响

目的　探讨肺表面活性物质 (PS)系统在急性油酸 (OA)性肺损伤时变化的作用机制及 β受体激动剂的干预作用。方法　 5 4只SD大鼠随机分成 3组 (每组 18只 ) ,即油酸致急性肺损伤组 (OA组 )、异丙肾上腺素治疗组 (ISO组 )和生理盐水对照组 (NS组 )。观察 1、2和 4h(每个时间点均 6只 ) ,测定动脉血氧分压 (PaO2 )、二氧化碳分压 (PaCO2 )、pH值和血清肺表面活性蛋白A(SP -A)浓度。右肺灌洗后 ,测灌洗液 (BALF)中总蛋白 (TP)、总磷脂 (TPL)、饱和磷脂酰胆碱 (DSPC)和SP -A浓度 ;测左肺上叶湿 /干重比值 (W/D) ,左肺下叶行病理检查。结果　与NS组比较 ,OA组各时间点BALF中TPL、DSPC/TPL和SP-A明显减少 (P <0 0 1) ,而W/D、TP及血清SP -A显著升高 (P <0 0 5或P <0 0 1) ,且OA组各时间点血清SP -A与同组BALF中SP -A呈负相关 ;ISO组BALF中TPL、DSPC/TPL和SP -A较OA组相同时间点明显上升 ,而W /D、TP及血清SP -A显著降低 (P <0 0 5或P <0 0 1)。结论　PS失活是急性油酸性肺损伤过程中的重要内在因素 ,β受体激动剂在一定程度上能减轻肺损伤的发生与发展。     

p65基因序列shRNA慢病毒载体构建及其作用功能的初步研究

Objective To obtain shRNA sequences that can stably block the expression of Nuclear Factor kappa- B (p65) in the prostate cancer cell line LNCaP and construct the lentivirus vector.And validate the gene function of p65 in the cell line. Methods According to p65 genetic information, we design siRNA1, siRNA2, siRNA3 those three siRNA sequences targeting the ods area of p65 gene and then form the corresponding four pairs of complementary single strand DNA of shRNA, including the sense strand and the antisense strand. The synthetic shRNA sequence was inserted into the empty pSIH1-H1-copGFP shRNA Vector, and after transfecting the prostate cancer cells , the inhibitory effect of p65 mRNA by different sequences was detected through real-time PCR, and the inhibitory effect of p65 protein expression was detected by Western-blotting. Thus we can obtain highly effective shRNA sequences in the inhibition of p65 in prostate cancer cells. MTT, flow cytometry, transwell were chosen to test the cell growth, migration and invasive power in vitro to compare the difference of the experimental group, control group and negative group. Results The third shRNA sequence had the best inhibitory effect and the inhibitory effect of p65 mRNA in prostate cancer cell line was 59 % and the protein was 81%. It's position locates in p65 (NM_021975 ) 1096-1113 and it's stemloop sequence is 5'-GATCCGCCCTATCCCTTTACGTCATTCAAGAGATGACGTAAAGGGATAGGGCTTTTTG-3'. After transfecting, the prostate cancer cell line had the low expression of p65 stably. Through MTT, we got the growth curve, which showed that the growth ability of experimental group was significantly decreased compared with the control group and the Logarithmic growth didn't appear in the first 96 hours. Flow cytometry test displayed that the percentage of G0-G1-phase cells in experimental group was 61.49%, and the control group was 44.89%, idle group was 41.52%, which was increasing oberviously. The S-phase cells in the experimental group was 28.58%, compared with the 47.36% and 46. 10% diminished. The results of transwell showed that the experimental group had 16. 5000±6. 62076 cells and the other two groups had 45. 6333 13. 54159 and 36. 8333±5. 68412 cells, which showed the invasive power of experimental group was significantly declined(P＜0.05).Conclusions It's successful to obtain shRNA sequences that can stably block the expression of p65 in the prostate cancer cell line LNCaP and construct the lentivirus vector. p65 can positively regulates the biological behavior of prostate cancer LNCaP cell line in the cell growth, migration and invasive power.     

中职学校英语分层走班教学模式的研究

分层教学是根据学生的身心发展所表现出的个体差异,因材施教,促进学生全面发展的一条有效途径。分层走班教学模式继承了传统班级授课制集体教学的优点,努力克服了它的弊端,最大限度地解决了中职生英语基础差异悬殊这一突出问题,增强了学生的学习兴趣和主动性,提高了他们的学习成绩。     

乳康口服液的急性毒性试验研究

目的观察乳康口服液的急性毒性,为临床安全用药提供科学依据。方法应用乳康口服液进行小鼠灌胃和腹腔注射急性毒性试验。结果小鼠灌胃乳康口服液最大耐受量（MTD）〉40mg.kg-1,日MTD〉120mg.kg-1,相当于人体用药日剂量的200倍;小鼠腹腔注射MTD〉25mg.kg-1,日MTD〉75mg.kg-1,相当于人体用药日剂量的125倍。结论乳康口服液用药安全范围较宽,无毒性作用。     

微小RNA let-7基因表达在膀胱癌生物学行为中的作用

目的 探讨膀胱癌组织中微小RNA(miRNA)表达在膀胱癌发生发展中的作用. 方法 Trizol法抽提9例膀胱癌患者癌组织及癌旁正常膀胱组织标本总RNA,分离与富集miRNA,随机选取2组miRNA标本与miRNA表达谱芯片杂交,采用LuxScan 3.0和SAM 2.1软件对芯片结果 进行图像数据分析.在差异性表达的miRNA中筛选出感兴趣的let-7基因,采用印迹杂交技术对9组miRNA标本中let-7基因进行验证. 结果 膀胱癌组织和正常膀胱组织标本中差异性表达有显著性意义的miRNA 71个,其中表达上调33个,表达下调38个;差异性表达有极显著性意义的miRNA(上调或下调≥4倍)26个,其中显著上调12个,显著下调14个.let-7基因验证结果 与芯片表达结果 一致,癌组织let-7基因表达吸光度值为479.6±228.2,正常膀胱组织为694.6±152.9,组间差异有统计学意义(P<0.05). 结论 膀胱癌组织和正常膀胱组织中存在差异表达的miRNA,let-7基因在膀胱癌的发生中可能起着抑癌基因的作用.