火棘果汁干预砷中毒小鼠肝功能损害指标的变化

目的:观察蔷薇科火棘属植物火棘浓缩果汁对小鼠砷中毒肝损害的干预效果。方法:实验于2005-10/12在广西右江民族医学院附属医院外科实验中心完成。①选用健康昆明小鼠60只,雌雄不拘。按随机摸球法将小鼠分为6组,每组10只:对照组,模型组,低、中、高剂量火棘果汁治疗组和硒治疗组。②应用400mg/L二砷酸钠溶液,按4mg/kg剂量对除对照组外其余小鼠皮下注射制备砷中毒模型,隔日1次,共15次。染毒结束后第7天,低、中、高剂量火棘果汁治疗组:灌胃2,4,10mL/(kg·d)火棘浓缩果汁(火棘鲜果实,采自广西隆林县,采用浸提法将火棘鲜果实进行分级、粉碎、榨汁、过滤、真空浓缩自制浓缩果汁;储存于4～5℃的冰箱内);硒治疗组:灌胃硒溶液100mg/(kg·d);对照组和模型组:灌胃等量生理盐水。各组均连续灌胃30d。③实验结束后取实验小鼠眼球血,按试剂盒说明书,用硫代巴比妥酸显色法测定丙二醛水平,用改良赖氏法测定丙氨酸氨基转移酶活性,用二硫代二硝基甲酸法测定谷胱甘肽过氧化物酶活性。④计量资料差异比较采用方差分析。结果:小鼠60只均进入结果分析。①高、中剂量火棘果汁治疗组和硒治疗组及对照组血清丙二醛水平和丙氨酸氨基转移酶活性均明显低于模型组(P<0.05),谷胱甘肽过氧化物酶活性明显高于模型组(P<0.05);低剂量火棘果汁治疗组血清丙氨酸氨基转移酶活性明显低于模型组(P<0.05),其他2项指标与模型组相近(P>0.05)。②高剂量火棘果汁治疗组血清丙二醛水平和丙氨酸氨基转移酶活性均明显低于低剂量火棘果汁治疗组(P<0.05),谷胱甘肽过氧化物酶活性明显高于低剂量火棘果汁治疗组(P<0.05);中剂量火棘果汁治疗组血清谷胱甘肽过氧化物酶活性明显高于低剂量火棘果汁治疗组,其他2项指标与低剂量火棘果汁治疗组相近(P>0.05)。结论:火棘果汁可能通过消除氧自由基,减轻砷中毒小鼠肝损害;高、中剂量火棘果汁作用效果明显优于低剂量。     

A549细胞线粒体DNA缺失模型建立及其放射敏感性变化

目的 建立肺癌A549细胞线粒体DNA（mtDNA）缺失模型（ρ⁰细胞）,观察其放射敏感性变化。 方法 用含微量溴化乙锭（EB）的特殊培养液持续培养正常的A549细胞（ρ⁺细胞）以获得mtDNA完全缺失的ρ⁰细胞,利用生长缺陷及PCR鉴定该细胞模型。利用克隆形成实验分别检测两种细胞的放射敏感性,利用碘化丙啶（PI）染色法及二氯荧光素双醋酸盐（DCFH-DA）染色法,分析两种细胞在6 MV X射线照射后的细胞周期及活性氧簇（ROS）变化情况。 结果 成功建立A549 ρ⁰细胞。ρ⁰细胞较ρ⁺细胞放射敏感性降低（t=12.57,P<0.01）。放射照射8 Gy后,ρ⁰细胞较ρ⁺细胞G₂期阻滞时间延长,G₂期细胞比例降低（t=6.82,P<0.01）,ρ⁰细胞ROS升高水平明显低于ρ⁺细胞（t=14.51,P<0.01）。 结论 ρ⁰细胞较ρ⁺细胞放射敏感性降低,其机制可能与放射照射后ROS产量降低及G₂期阻滞延长有关。     

氧嗪酸钾联合不同造模剂对小鼠高尿酸血症的尿酸值的影响

目的 探讨氧嗪酸钾联合不同造模剂对小鼠高尿酸血症(HUA)尿酸值的影响,为早期HUA的防治和药物筛选提供参考.方法 采用300 mg/kg氧嗪酸钾、500 mg/kg尿酸、500 mg/kg次黄嘌呤、250 mg/kg乙胺丁醇多个造模剂,对ICR小鼠进行HUA造模,通过不同时间段取血测定尿酸值,比较不同造模因素对血尿酸...     

慢性心力衰竭专病护士团队的组建及实践

目的 探讨专病护士团队在慢性心力衰竭患者治疗护理中的作用。方法 将2017年6～12月收治的300例慢性心力衰竭患者作为对照组,实施常规治疗护理和出院后随访;2018年1～6月收治的300例慢性心力衰竭患者作为观察组,由专病护士团队针对患者进行评估、跟踪,筛查临床前期患者,对临床期患者制定个性化的健康指导和出院后的团队延续护理,帮助终末期患者选择合适的治疗方案、实施急救措施、心理支持及社会援助;同时重视质量控制和专病护士的考评和管理,协助心力衰竭治疗中心和示范基地的建设。于干预6个月后比较两组疾病自我管理能力评分、再住院情况,以及医护患满意度。结果 干预6个月后,观察组疾病自我管理能力、患者对护理工作满意度显著高于对照组,再住院率和再住院时间显著降低和缩短（均P<0.01）。医生、护士对专病护士团队的工作满意度较高。结论 专病护士团队针对慢性心力衰竭不同阶段进行分工分期管理,可以提高患者生活质量和临床结局,有利于护士职业发展。     

食道超声探头在消毒供应中心处理的规范化管理

总结了食道超声探头在消毒供应中心处理的规范化管理过程,主要包括食道超声探头在接收、消毒、发放环节中,防止探头出现碰撞、摔损等问题;并对26名工作人员进行接收发放、清洗消毒及包装的培训,保障了食道超声探头清洗效果,有效地预防以及控制感染。认为食道超声探头在消毒供应中心处理过程中,只有实施规范化、专业化管理,才能确保其使用性能以及灭菌质量符合标准要求,才能为手术患者提供安全保障。     

含量测定与特定（指纹）图谱分析相结合方法用于枳壳药材质量分析

目的:以枳壳为对象,研究准确反映药材内在整体质量的分析方法。方法:采用色谱分析法建立枳壳的化学特定(指纹)图谱,同时选择关键成分柚皮苷建立含量测定方法,从特定(指纹)图谱相似度与关键成分含量限度两方面同时入手,评价枳壳药材质量。色谱特定(指纹)图谱条件为:Agilent Hypersil ODS柱(4.0 mm×250 mm,5μm),0.5％醋酸-甲醇为流动相,线性梯度洗脱程序:0 min,流动相比例为80:20;10 min,60:40;35 min,30:70;50 min,0:100;55 min,0:100。流速1 mL·min-1,柱温30℃,检测波长320 nm。柚皮苷含量测定色谱条件为:Agilent Hypersil ODS柱(4.0 mm×150 mm,5μm),流动相为0.3％醋酸-甲醇(65:35),流速1 mL·min-1,柱温30℃,检测波长280 am。结果:不同产地及同产地不同批次枳壳药材柚皮苷的含量在3.19％-5.50％之间,有一定差异;相同产地枳壳的特定(指纹)图谱相似度较高,不同产地枳壳的特定(指纹)图谱相似度较低。结论:特定(指纹)图谱与关键成分含量测定相结合的分析方法能更全面反映枳壳药材整体质量,是一种有效的中药材质量分析方法。     

七氟醚对感染性休克大鼠全身炎性反应及心肺功能的影响

Objective To investigate the effects of sevoflurane on the systemic inflammatory response and cardiopulmonary function in septic shock rats. Methods Thirty-two SD rats, 8-10 months old, weighing 250-300 g, were randomly divided into 4 groups (n = 8 each): sham operation group (group S), cecal ligation and puncture (CLP) induced septic shock group (group CLP) , sevoflurane I group (group SEV, ) and sevoflurane II group (group SEV,). The abdomen was opened but CLP was not performed in group S. The septic shock was induced by CLP as described by Baker et al. Group SEV, and SEV, inhaled 2.4% sevoflurane for 30 min at 1 h and 3 h after the successful establishment of the model respectively. At 1, 3 and 5 h after septic shock, MAP and HR were recorded and arterial blood samples were taken for blood gas analysis and determination of plasma concentrations of TNF-α, IL-1, MDA and NO. The left ventricular end-diastolic diameter (LVEDD), left ventricular end-systolic diameter (LVESD), left ventricular fractional shortening (LVFS) and cardiac output (CO) were also detected 5 h after septic shock. The animals were killed after the detection of cardiac function. The lungs were removed for determination of W/D lung weight ratio and Evans blue (EB) content. The tissues from the heart, lung, liver and kidney were taken for detection of NF-kB activity by electrophoretic mobility shift assay (EMSA) ResultsMAP was significantly lower, HR higher, LVEDD, LVESD, LVFS, CO, pH value, PaO2 and PaCO2 lower, and W/D lung weight ratio, EB content, plasma concentrations of TNF-α, IL-1, MDA and NO, and NF-kB activity in the heart, lung, liver and kidney tissues higher in group CLP, SEV, and SEV2 than in group S (P < 0.05). NF-kB activity in the heart, lung, liver and kidney tissues and plasma concentrations of TNF-α, IL-1, MDA and NO were significantly lower in group SEV, than in group CLP and SEV2 ( P < 0.05 ), but no significant differences were found in the other indices between group SEV, and CLP and between group SEV1 and SEV2 ( P > 0.05). Conclusion Inhalation of 2.4% sevoflurane for 30 min 1 h after septic shock can inhibit the systemic inflammatory response slightly, but can not improve the cardiopulmonary function in rats with CLP-induced septic shock.     

健康体检人群幽门螺杆菌感染与白蛋白尿的相关性CSCD

Objective To investigate the correlation between Helicobacter pylori (H.pylori) infection and albuminuria inphysical examination population. Methods It was a cross‑sectional study. All the adults who received physical examination and underwent 13C‑urea breath test at the Physical Examination Center in Tongji Hospital in 2021 were selected as the study subjects. General data (such as demographic information and past medical history) were documented. The physical measurement and blood biochemical indicators were checked too. Multivariate analysis was used to analyze the relationship between H. pylori infection and albuminuria. Results A total of 30 311 subjects were included in this analysis. There were 17 123 males and 13 188 females with an age of (44.51±12.17) years. The positive rate of H. pylori infection was 27.3%. The incidence of albuminuria in subjects with H. pylori infection was 6.7%, and it was 6.1% in the subjects without H. pylori infection (P=0.031). After adjusting for the confounding factors such as gender, age, diabetes and hypertension, H. pylori infection was independently associated with the risk of albuminuria (odds ratio (OR)=1.133, 95%CI: 1.018-1.261, P=0.022). Conclusion H. pylori infection is positively correlated with the occurrence of albuminuria in the physical examination population. © 2021 Chinese Medical Journals Publishing House Co.Ltd.