原发性乳房结核1例

患者女,20岁。未婚,2年前发现左侧乳房有一包块,不痛,不畏寒发烧,未行治疗。近3个月来乳房包块增大,药物治疗无效住院。既往身体健康,否认结核病史。体查:体温37℃,脉搏70次/min,呼吸20次/min,血压110/80mmHg(1mmHg=0.133kPa)。心肺(-),腹平坦、软,肝脾肋下未触及,脊柱四肢无畸     

胃黏膜上皮细胞的分离及其质膜蛋白质的初步分析

目的 探讨分离胃黏膜上皮细胞的方法及对其细胞膜蛋白质的初步分析.方法 采用0.1%的胃蛋白酶溶液消化掉附在胃黏膜上的黏液,刮取黏膜,用机械法将黏膜剪碎,过滤,离心,从而获得胃黏膜上皮细胞;用Triton-114方法提取细胞质膜蛋白质,SDS-PAGE电泳,并对其中的几个条带质谱分析.结果 成功获得了胃黏膜上皮细胞;胃黏膜上皮细胞质膜蛋白质得到了较好的分离;SDS-PAGE电泳细胞膜蛋白质得到了清晰的条带;用质谱分析条带获得了一些细胞膜蛋白信息.结论 利用物理和化学相结合的方法可以分离得到胃黏膜上皮细胞,成功提取胃黏膜上皮细胞质膜蛋白质并进行了初步鉴定.     

肠外瘘对患者口腔黏膜细胞凋亡和增殖的影响

目的：通过测定口腔黏膜细胞的凋亡率和增殖率来评估肠外瘘对患者营养状况的影响。方法：选取25例肠外瘘住院患者为实验组,同时设12例健康人作对照组。用流式细胞术亚G1峰法和增殖抗原Ki67标记法分别检测口腔黏膜细胞的凋亡率和增殖率。通过统计学分析两组之间口腔黏膜细胞凋亡率和增殖率的差异。并检测实验组的血液生化指标来比较该实验方法与传统的营养评估方法的优缺点。结果：肠外瘘患者口腔黏膜细胞的凋亡率和增殖率明显低于健康对照组,肠外瘘患者口腔黏膜细胞的凋亡率和增殖率的降低早于血液生化指标的改变。结论：口腔黏膜细胞凋亡率和增殖率的变化与肠外瘘导致的营养障碍密切相关,其检测可以作为一种新的灵敏、可靠的营养评估方法。     

Proliferation characteristics of CD133+ cell population in colorectal cancer

In this study,CD133+ subpopulations were isolated from 41 primary colorectal cancer tissues,the proliferation and cell cycle distribution of the cells were examined without in vitro expansion,and then compared to those of cell lines.The detection of CD133 in colorectal cancer tissues,isolation of CD133+ and CD133-epithelial subpopulations,Ki-67/DNA multiparameter assay and cell volume analysis were flow cytometrically conducted.The results showed that Ki-67 expression was correlated with CD133 level in primary cancer tissues,while cell cycle G 2 /M phase distribution or clinicopathological characteristics was not.In addition,the CD133+ cells showed larger cell volume and higher Ki-67 expression as compared with CD133-cells.But there was no statistically significant difference in G 2 /M phase distribution between the two subpopulations.Our results demonstrated that the CD133+ subpopulation in colorectal cancer tissue contained more actively cycling and proliferating cells,which was not correlated to clinicopathological factors but might contribute to tumor progression and poor clinical outcome.