直肠内括约肌切除治疗先天性巨结肠的改进

直肠内括约肌切除治疗先天性巨结肠的改进翁一珍，王果，郭先娥１９８９年以来本科采用经肛门直肠内括约肌切除术治疗先天性巨结肠症（ＨＤ）１６例，男１３例，女３例。手术年龄２７天～７岁（３个月内９例）。全组病例均有典型病史。钡灌肠示痉挛段距肛门６ｃｍ以下（相...     

小鼠Connexin 31基因组克隆的筛选及荧光原位杂交定位

Connexin 31 (Cx31 )是人类遗传性疾病基因 ,利用小鼠Cx31基因的cDNA序列探针从小鼠的基因组文库筛选出Cx31的gDNA克隆 ,并用荧光原位杂交技术将其定位于小鼠 4号染色体的中部 ,为小鼠Cx31的基因结构和功能的研究以及转基因小鼠和基因剔除小鼠的研究打下了良好的基础     

肾移植受者CYP3A5基因多态性对术后他克莫司药物代谢的影响

目的 研究肾移植受者细胞色素P450 3A(CMP3A)酶系中的CYP3A5基因多态性对术后他克莫司(Tac)药物代谢的影响,并探讨测定CYP3A5基因型的方法 .方法 选取97例肾移植术后常规使用Tac+吗替麦考酚酯+泼尼松三联免疫抑制方案的受者.用焦磷酸测序法测定受者CYP3A5基因型;用均相酶扩大免疫分析法(EMIT)测定受者全血Tac浓度谷值(C0);比较不同基因型的受者肾移植术后第7天、第14天、1个月、3个月和6个月时的Tac C0,与Tac校正剂量的比值(Tac G0/Tac校正剂量).结果 焦磷酸测序法能够快速准确的测定肾移植受者CYP3A5基因型;97例肾移植受者CYP3A5基因型分别为:CYP3A5+1/*1型17例(17.5%),CYP3A5* 1/*3型35例(36.1%),CYP3A5*3/*3型45例(46.4%).CYP3A5*1/*1型和CYP3A5*1/*3型受者肾移植术后Tac G0/Tac校正剂量均显著低于CYP3A5*3/*3型受者.结论 肾移植受者CYP3A5基因型对Tac药物代谢具有显著的影响.采用焦磷酸法测定肾移植受者CYP3A5基因多态性,有助于确定不同个体移植术后理想的Tac起始剂量,实现Tac个体化用药.     

内括约肌部分切除治疗肛门内括约肌失弛缓症

目的评价内括约肌部分切除术治疗肛门内括约肌失弛缓症的疗效。方法回顾性分析 6例该病患儿的临床表现、钡剂灌肠X线检查、直肠肛管测压及黏膜乙酰胆碱酯酶免疫组化染色结果 ,并对所有患儿进行远期随访。结果本组患儿均表现为顽固性便秘 ,钡剂灌肠X线检查可无狭窄段 ,直肠肛管松弛反射消失 ,黏膜活检乙酰胆碱酯酶组化染色阴性 ,术后病理检查肌丛中有神经节细胞。患儿术后均顺利出院 ,并随访 2～ 7年 ,均能自行排便、控便。结论内括约肌部分切除术治疗肛门内括约肌失弛缓症可取得满意的远期疗效。     

先天性巨结肠症血清及肠壁内锌铜铁含量测定

文献报导先天性巨结肠症为一多因素遗传性疾病,且有地区种族的差别。长期以来,人们对其病理生理、诊断方法及治疗方法进行了大量的研究,但是对患儿血清及病变狭窄段肠管及近端正常肠段的肠壁内某些微量元素含量,尚未见有述及。为了探讨微量元素与巨结肠症之间关系,作者进行了这方面的测试,以期了解彼此间有无差异。材料与方法一、1986年9月～1987年9月共收集巨结肠手术病儿血清及肠壁标本。年龄最小11月,最大5岁。男18例,女4例。对照组10例系住院非消化道疾病患儿(如疝、尿道下裂、鞘膜积液)。二、标本收集:按去离子要求,采集术前空腹静脉血,经双离心除尽红细胞后抽吸其血清存于去离子试管内待测。手术时切取狭窄段及近心端正常肠管全层各1×1cm~2,用生理盐水及双蒸水冲洗干净,置烤箱内烤干后放于密封干燥器内。三、血清经处理后以日立180—80型原子吸收光谱仪测定。     

先天性巨结肠同源病:临床诊断与手术指征的确立

Objective To determine the diagnostic criteria and surgical indications of allied diseases of Hirschsprung's disease (HAD) in children. Methods During the period from Jun. 2007 to Dec. 2009,65 consecutive patients were performed "radical surgery for megacolone" by this surgery team. Based on the clinical symptoms,anorectal manometry, AChE staining of rectal mucosa and barium enema,the preoperative suspected diagnosis of Hirschsprung's disease (HD) or HAD was made.Surgical indications were determined once the suspected HD diagnose was made. On the patients withsuspected HAD, strictly conservative treatments should be carried out for at least 3 months. If conservative treatments were ineffective or the symptoms were recurrent, surgical indication of HAD patients could be determined. Results The preoperative diagnosis was 38 patients with HD and 27 with HAD.However, the final pathologic diagnosis was 36 with HD and 29 with HAD. The misjudged preoperative diagnosis was made in 10. 5% (4/38) HD patients and 7. 4% (2/27) HAD patients. The HAD patients included 22 (75. 9%) hypoganglionosis (HG) cases and 7 (21.1 %) intestinal neuronal dysplasia (IND) cases. Among the 27 HAD patients, 27 (93. 1%) had anorectal inhibitory reflex, and varied and delayed reflection waveforms. AChE staining was positive in 12 (41.1%) patients. The results of anorectal manometry and AChE staining of the HAD patients were significantly different from those of the HD patients (P＜0. 01 ). Barium enema of the HAD patients appeared severe expanded rectum which occupied more than 90% space of the pelvic cavity. Redundant sigmoid colon was also found in more than 90% patients. All HAD patients had 24-hour barium retention. Subtotal colectomies were performed on 9 (31. 0%) HAD patients,and 8 (22. 2%) HD patients. Conclusions Anorectal manometry, AChE staining and barium enema are still important in determining the diagnosis and surgical indications for HAD. The efficacy of 3 months or longer conservative treatment is crucial to determine the surgical indications for HAD.     

基于个性化医学的中医体质辨识与干预智能平台

根据中医体质辨识理论和因人制宜的个性化医学理论,利用微博接口建立认证,为用户提供中医体质测评系统,并根据不同体质提供相应建议.在此基础上进行个性化推荐模型设计,详细阐述其设计原则、用户流程,并对应用效果进行分析.     

血清促胃液素水平的测定在消化道肿瘤诊断中作用的研究

近年来,消化道肿瘤与胃肠道激素的关系日益受到重视。促胃液素(gastrin,Gas)对消化道肿瘤的发生、发展等有促进作用。本应用放射免疫法对消化道良性病变33例、消化道肿瘤37例及肿瘤术后患19例血清Gas的含量作了检测。现将结果报道如下。     

人长链非编码RNA基因H19克隆、表达载体构建及对MCF-7细胞增殖的影响

目的克隆人长链非编码RNA H19基因,构建表达载体,研究H19表达对MCF-7增殖的影响作用。方法制备乳腺癌MCF-7细胞总RNA,RT-PCR扩增长链非编码RNA H19全长序列,分子克隆至pc DNA3.1(-)真核表达载体;分别转染HEK-293T和COS 7细胞并行Real-time q PCR验证载体构建是否成功;转染H19表达载体入MCF-7细胞株,转染0、24和48 h后行MTS检测H19表达,同时设计siRNA小分子片段干扰H19的表达,观察对MCF-7细胞增殖活性的影响。结果成功克隆和构建了h H19-pc DNA3.1(-)表达载体;转染入MCF-7细胞48h后,H19过表达,MTS检测H19表达载体组吸光度明显高于空载体组和空白对照组;而转染H19 siRNA小分子片段后能干扰其表达,同时抑制细胞增殖。结论 H19过表达能够促进乳腺癌MCF-7细胞增殖。