爆炸冲击伤诊治中值得关注的几个问题

随着我国工业化进程以及社会不稳定因素的长期存在,意外爆炸事件时有发生并渐呈增多趋势。爆炸冲击伤伤情特点也表现出新的规律和伤情转归特点。为此,本文立足和平时期爆炸冲击伤诊治情况,回顾了爆炸冲击伤类别与伤情特点,分析爆炸冲击伤伤情与距离的内在联系,并对爆炸冲击伤复合因素在伤情诊治中的权重予以探讨。旨在结合爆炸冲击伤循证医学证据与经验评估,尝试实现爆炸冲击伤精准评估与个体化诊治的可能性,并从爆炸冲击伤诊治角度对防护策略提出新的需求,为和平时期爆炸冲击伤的规范化诊治提供借鉴和启示。     

大鼠急性肺损伤病理变化的形态计量研究

目的 用体视学方法定量研究大鼠经胸部撞击复合内毒素所致急性肺损伤(acute lung injury,ALI)模型的组织病理学特点及变化规律. 方法 12只SD大鼠按随机数字表法分为正常对照组、ALI致伤1,2,7d组,每组3只.各组取肺组织行HE染色,分别测试肺实质百分比、肺泡间隔厚度、肺泡截面积及肺泡腔内炎性细胞计数. 结果 ALI致伤1,2d组肺泡腔内炎性细胞计数分别为(13.08 ±6.60)个、(26.81±16.86)个,正常对照组为(0.89±0.78)个,致伤组均较对照组明显增加(P＜0.01).ALI致伤1,2,7d组肺实质百分比分别为(23.77±3.91)％、(27.32±5.95)％、(44.57±6.46)％,正常对照组为(19.44±2.48)％,致伤组均较对照组明显增加(P＜0.01).ALI致伤1,2,7d组肺泡间隔分别为(3.50±2.18) μm、(7.67±7.14) μm、(17.23±13.08) μm,正常对照组为(2.87±1.75) μm,致伤组均较对照组逐渐增厚(P＜0.05).ALI致伤1,2,7d组肺泡截面积分别为(1 653.90±950.77)μm2、(1 328.15±802.30) μm2、(1 055.20±1 002.75) μm2,正常对照组为(1 641.30 ±906.60)μm2,致伤组均较对照组逐渐减少(P＜ 0.05). 结论 大鼠经胸部撞击后复合静脉注射内毒素可建立病理形态上符合ALI的动物模型.通过体视学方法定量检测可反映ALI的组织病理学特点及变化规律.     

Silencing effect of recombinant plasmids PPARγ-pSUPER-EGFP on the expression of peroxisome proliferator-activated receptorγ

Peroxisome proliferator-activated receptor γ( PPARγ), belonging to the nuclear steroidreceptor superfamily, plays an important role in1,2lipid metabolism and glucose homeostasis.1,2Recently,some studies have shown that PPARγcan specificallysuppress the gene expression of pro-inflammatorymediators through transactivation, as a checkpoint incontrolling the inflammatory responses. PPARγ iswidely distributed on macrophages, dendritic cells, Tcells, B cells and so on. Its expression is dra…     

中药配伍减毒研究的新思路

中药配伍减毒涉及的复杂反应过程制约了对配伍减毒机制的深层次解构。阐明配伍减毒机制是解决中药毒性问题的关键。"效毒二重性"决定了有毒中药"效-毒交集"特征,交集调控可反映配伍减毒复杂过程。网络药理学的出现,为开展有毒中药毒性成分筛查、致毒机制和减毒效应等研究提供了新的手段。在回顾中药毒性研究实践的基础上,系统阐述网络药理学在中药配伍减毒研究中的应用策略,并以附子为例探讨其可行性。     

Construction and identification of a vector expressing TRAM siRNA in mammalian cells

Objective：To construct and identify a vector expressing TRAM siRNA in mammalian cells.Methods :It was constructed that the vector named R-pSUPER-EGFP used to transcribe functional TRAM siRNA. Two of pair 64 nt TRAM gene specific target sequences were inserted into the downstream of the H1 promoter. The recombinants were transformed into E. coli JM109, and finally their veracity was confirmed by double cutting with the enzymes and sequencing. R-pSUPER-EGFP was transfected into RAW264. 7 cell by using LipofectamineTM2000, and the expression of TRAM was detected by Western blotting. Results .. Two different recombinant plasmids containing corresponding TRAM gene specific target sequences were constructed, transfected into RAW264.7 cell line successively, which can specifically restrain expression of TRAM protein. Conclusion :The optimizing method in constructing the recombinant vector serves other plasmid-based RNA interference research. Therefore, the recombinant vectors establish the basis for research on the function of TRAM gene.     

科研团队的心理契约与绩效管理

心理契约对团体、成员配合和提高整体绩效有积极的作用。将心理契约的管理思维引入科研管理是适应科学技术发展的需要,也有助于在更深层次上和更广范围内激励团队创新。本文应用心理契约思维对加强973项目组织领导、激发团队成员的创造力和工作动力、促进科研群体创新和组织管理创新等方面进行了积极探索。     

Profiles of hippocampal neuron activity during auditory discrimination cognition in guinea pigs

Objective： To clarify the firing characteristics of the hippocampal pyramidal cells and interneurons in the auditory discrimination cognition. Methods：Thirteen guinea pigs were studied by the paired （active cognition group, n=10） or unpaired （passive cognition group, n= 3） training with 1 kHz （CS^＋）and 500 Hz tones （CS^-） and the air puff （US） applied 250 ms after the CS^＋ onset. Results： In active group, 32 pyramidal cells showed exciting response to the CS^＋ tone, 16 cells inhibited response and 4 cells revealed no response to the high frequency tone and 18 interneurons almost unchanged. In passive group, the pyramidal cells responded to the tone casually and 10 out of the 13 interneurons remained invariably. Conclusion：The result suggests that the pyramidal cells play a major role in coding auditory information by the networks, and the interneuons may modulate it via forward and feedback.     

中医药数据挖掘系统的构建与应用

目的 针对中医药数据的特点和数据挖掘的需求,开发中医药数据挖掘系统(TCM Miner).方法 TCM Miner基于中医药术语词表,围绕中医药数据挖掘工作中的数据清洗、集成、变换、选择等需求,分别构建用于数据清洗的数据拆分与合并、正异名替换、文本内容抽取、矩阵转换、中医药文本ETL等功能模块,用于数据挖掘的关联关系挖...     

跑台运动对成年脊髓损伤小鼠运动功能及运动皮质mTOR信号通路的影响

目的 观察跑台运动对成年脊髓损伤小鼠运动功能及运动皮质哺乳动物雷帕霉素靶蛋白（mTOR）信号通路的影响。方法 30只C57BL/6J小鼠随机分为假手术组、模型组与跑台运动组,每组10只。所有小鼠进行跑台运动预训练3 d后,模型组和跑台运动组小鼠予以C₅背侧皮质脊髓束、左侧皮质脊髓束及红核脊髓束钳夹损伤,假手术组仅剔除椎板。跑台运动组伤后7 d开始跑台运动,5 d/周,共4周。采用BMS评分、水平楼梯实验和圆筒攀爬实验评估小鼠运动功能恢复情况;Western blotting检测小鼠运动皮质脑源性神经营养因子（BDNF）、胰岛素样生长因子1（IGF-1）的表达,以及酪氨酸激酶受体B（TrkB）、胰岛素样生长因子1受体（IGF-1R）、蛋白激酶B（Akt）、核糖体S6蛋白（S6）的磷酸化水平;免疫荧光染色检测运动皮质神经元S6的磷酸化水平,评估皮质mTOR信号通路激活情况。结果 伤后模型组与跑台运动组小鼠BMS评分出现短暂下降（_P<0.0001）,并于伤后7 d恢复至基线水平。伤后3 d、1周、3周、5周,模型组和跑台运动组左前、左后肢错误...