TLR2和TLR9在生殖器疱疹患者外周血单一核细胞的表达

Objective To study the expression level of TLR2 and TLR9 in peripheral blood mononuclear cells(PBMC)from patients with genital herpes(GH).Methods Venous blood samples were collected from 20 patients with GH and 10 normal human controls.Flow cytometry was used to detect the expression levels of TLR2 and TLR9 in PBMC from these samples.Serum levels of IL-6,IL-10 and TNF-α were measured by ELISA.Results The percentage of PBMCs expressing TLR2 or TLR9 was significantly higher in patients than in normal human controls(18.88%±15.31% vs 7.49%±1.55%,85.29%±3.15% vs 22.23%±2.38%,both P<0.05).Compared with the normal human controls,increased serum levels of IL-6 and IL-10 were observed in patients(91.25±3.22 pg/mL vs 6.65±1.27 pg/mL,3.94±0.57 pg/mL vs 2.13±0.24 pg/mL,both P<0.05),together with a decrease in the serum level of TNF-α(12.07±1.27 pg/mL vs 23.06±4.62 pg/mL).The expression levels of TLR2 and TLR9 significantly correlated with serum levels of IL-6 and IL-10 in patients with GH (all P<0.01).Conclusion The production and release of sefum IL-6 and IL-10 might be mediated by TLR2 and TLR9 in patients with genital herpes.     

胰岛与Sertoli细胞体外共同培养

目的 研究胰岛体外长期培养的新方法。方法 采用绿色荧光蛋白（GFP）标记成年SD大鼠胰岛，与Sertoli细胞共同培养20周，应用形态学方法和透射电镜观察胰岛细胞单独培养和共同培养的生长特性；放射免疫法测定胰岛素分泌量。结果 胰岛单独培养3周。细胞存活率显著下降，胰岛阳性细胞数目减少，胰岛素24h累积分泌量和对葡萄糖刺激的反应性下降，大部分胰岛β细胞超微结构破坏，分泌颗粒减少；与Sertoli细胞共同培养的胰岛存活时间显著延长，细胞存活率和胰岛素阳性细胞数目较单独培养显著增加，胰岛素分泌量始终处于高水平状态，培养20周胰岛β细胞超微结构基本正常，结论 大鼠胰岛与Sertoli细胞共同培养可以促进胰岛生长，显著延长存活时间，是一种新的体外长期培养胰岛的方法。