肝吸虫病患者阿苯达唑治疗前后血清TNF-α、IL-6和IL-8的变化及意义

目的 研究阿苯达唑治疗肝吸虫病前后患者血清细胞因子TNF-α、IL-6和IL-8水平的变化情况，探讨其在肝吸虫致病机制中的作用及临床意义。方法 以健康人作对照，健康人和肝吸虫病患者在口服阿苯达唑治疗前、后4周分别于清晨空腹采集静脉血，用ELISA方法检测患者血清TNF-α、IL-6和IL-8水平。结果 治疗前肝吸虫病患者血清TNF-α、IL-6和IL-8水平升高，与健康对照组比较有统计学意义。口服阿苯达唑治疗后，患者血清TNF-α、IL-6和IL-8水平下降，与治疗前比较有统计学意义。结论 肝吸虫病患者血清细胞因子水平异常，免疫功能下降，应用阿苯达唑治疗后免疫抑制得到改善；阿苯达唑对于治疗肝吸虫病具有较好的疗效。     

囊虫病患者血清中C_3和循环免疫复合物的检测

本文检测了37例囊虫病患者血清中 C_3和循环免疫复合物的水平。结果表明,患者血清中 C_3均值明显低于正常人的测定值(P<0.01),而循环免疫复合物的水平明显增高(P<0.01)。二者呈显著的负相关。提示在人体囊虫病发病过程中,抗原抗体复合物能够激活补体,从而导致补体介导的免疫反应的发生。     

无创性肢体缺血预适应对糖尿病大鼠心肌缺血/再灌注损伤的保护作用及缺血下肢的影响

目的观察无创性肢体缺血预适应对糖尿病下肢缺血大鼠心肌缺血/再灌注损伤的保护作用及对缺血下肢的影响。方法将成功建模的糖尿病下肢缺血大鼠随机分为2组(每组27例):心肌缺血/再灌注组(A组)及预适应组(B组)。A组大鼠冠状动脉左前降支结扎30 min后开通2 h;B组大鼠缺血下肢行结扎5 min后开通5 min,共3次,连续3 d,第4天同A组实验。观察比较两组大鼠心电图ST段变化程度、肌酸激酶同工酶(CK-MB)值、缺血心肌质量与左心室质量的比值(AAR/LV)、梗死心肌质量与左心室质量的比值(IA/LV)、梗死心肌质量与缺血心肌质量的比值(IA/AAR)以及缺血下肢与健肢的血流值比率(R/L)。结果与A组相比,B组ST段抬高幅度、CK-MB值、IA/LV、IA/AAR值明显降低,R/L有降低趋势,但差异无统计学意义(P0.01)。结论无创性肢体缺血预适应能明显降低大鼠心肌缺血/再灌注时的ST段抬高幅度、CK-MB值及心肌梗死面积,且对已发生缺血的下肢无显著影响。     

阿苯达唑联合黄芪对华支睾吸虫感染大鼠肝功能异常的疗效观察

Objective To observe the levels of alanine aminotransferase(ALT), total bilirubin(TBIL), hyaluronic acid (HA), procollagen type Ⅲ aminoterminal peptide (P Ⅲ NP) and larninin (LN) in the sera of rats infected with Clonorchis sinensis (C. sinensis) after treatment of albendazole combined with milkvetch root. Methods Thirty-two healthy adult Wistar rats were randomly divided into four groups with 8 in each based on body mass: control group, non-treatment group, Albendazole group(ALB group) and albendazole combined with milkvetch root group(ALB+MR group). The rats in non-treatmen, ALB and ALB+MR groups were infected orally with metacercariae of C. sinensis 50 per rat. The rats in control group were mock-infected with saline. The rats in ALB group were treated to each rat with 50 mg/kg alhendazole for 5 days, and ALB+MR groups were given to same treat with albendazole, meanwhile each rat injected with 800 mg/kg milkvetch root intraperitoneally for 30 days. All rats were killed after infestation 14 weeks and their sera samples were collected to detect ALT, TBIL, HA, PⅢNP, LN. Results There were statistically significant differences in the levels of ALT and LN in the sera of rats between groups(F=31.40,11.82, P<0.01). Compared with control[ (47.88±4.88)U/L, (51.20±4.12)μg/L], the levels of ALT and LN in rats in non-treatment group [(85.50±9.65)U/L, (64.20±4.18) μg/L] and ALB group [(65.29± 7.78) U/L, (58.23±2.55) μg/L] were significantly increased (P<0.05). Compared with non-treatment group, the levels of ALT and LN in rats in ALB group and ALB+MR groups[(50.25±9.29)U/L, (53.68±5.63)μg/L] were significantly decreased(P<0.05), and they decreased more obviously in ALB+MR group (P<0.05). There were statistically significant differences in the levels of TBIL, PⅢNP and HA in the sera of rats between groups (χ2=15.309,21.418,19.759, P<0.01). Compared with control[(0.700±0.350)μmol/L, (26.085±4.075)μg/L, (81.935±42.550)μg/L], the levels of TBIL, PⅢNP and HA in rats in non-treatment group(2.400 μmol/L, 46.220 μg/L,310.885 μg/L) and ALT group(1.200 μmol/L,36.540 μg/L, 178.010 μg/L) were significantly increased(P<0.05). Compared with non-treatment group, the level of TBIL in ALT+MR group(0.750 μmol/L), the levels of pⅢNP and HA in ALT and ALT+MR group(30.470,100.240 μg/L) were significantly decreased(P< 0.05). The levels of TBIL, PⅢNP and HA decreased more obviously in ALB+MR group(P<0.05). Conclusions The liver function in rats infected with C. sinensis is abnormal. The liver function and fibrosis are improved after treatment with albendazole or albendazole combined with milkvetch root. The treatment of albendazole combined with milkvetch root is more effective.     

哈尔滨市屠宰场从业人员旋毛虫感染的检测与分析

旋毛虫的宿主选择性极差 ,可感染人及 15 0多种动物 ,引起严重的肉食源性人畜共患寄生虫病 [1 ] 。我国是旋毛虫感染较严重的国家之一 ,到 1996年底 ,已有 15个省 (自治区 )发生旋毛虫病和旋毛虫病的暴发流行 [2 ] 。牡丹江市猪血清旋毛虫抗体阳性率为 10 .4 1% (38/ 36 5 ) [3] 。房其美 [4 ] 对郑州市屠宰场从业人群进行调查 ,其血清旋毛虫抗体阳性率为 9.82 % (2 8/ 2 85 )。为了解哈尔滨市屠宰场从业人员旋毛虫感染情况 ,作者应用 EL ISA法对屠宰场从业人员和其他职业者进行了血清旋毛虫抗体的检测与分析。1　材料和方法1.1　检测对象…     

阿苯达唑联合黄芪对华支睾吸虫感染大鼠肝功能异常的疗效观察

目的 观察阿苯达唑联合黄芪对华支睾吸虫感染大鼠肝功能异常的疗效.方法 健康成年Wistar大鼠32只,按体质量随机分为对照组、未治疗组、阿苯达唑组和阿苯达唑+黄芪组.每组8只.未治疗组、阿苯达唑组和阿苯达唑+黄芪组大鼠给华支睾吸虫囊蚴50个/只灌胃,对照组大鼠给生理盐水灌胃:阿苯达唑组大鼠在感染后10周每日每只给阿苯达唑50 mg/kg灌胃,连续5 d;阿苯达唑+黄芪组大鼠在给阿苯达唑的同时腹腔注射黄芪注射液800 mg/kg,连续30 d.于感染后14周采集血样,检测血清丙氨酸转氨酶(ALT)、总胆红素(TBIL)、透明质酸酶(HA)、层粘连蛋白(LN)和Ⅲ型前胶原(PCⅢP)水平.结果 大鼠血清ALT和LN水平组间比较,差异有统计学意义(F值分别为31.40、11.82,P<0.01).血清ALT和LN水平,未治疗组[(85.50±9.65)U/L、(64.20±4.18)μg/L]、阿苯达唑组[(65.29±7.78)U/L、(58.23±2.55)μg/L]较对照组[(47.88±4.88)U/L、(51.20±4.12)μg/L]明显升高(P<0.05);阿苯达唑组和阿苯达唑+黄芪组[(50.25±9.29)U/L、(53.68±5.63)μg/L]较未治疗组明显下降(P<0.05),阿苯达唑+黄芪组下降较阿苯达唑组更明显(P<0.05).大鼠血清TBIL、PⅢNP和HA组间比较差异有统计学意义(χ2值分别是15.309、21.418、19.759,P<0.01).血清TBIL、PⅢNP、HA水平,未治疗组(2.400 μmol/L、46.220 μg/L、310.885 μg/L)、阿苯达唑组(1.200 μmol/L、36.540 μg/,L、178.010 μg/L)较对照组(0.700 μmol/L、26.085 μg/L、81.935 μg/L)明显升高(P<0.05).大鼠血清TBIL水平,阿苯达唑+黄芪组(0.750 μmol/L)较未治疗组明显降低(P<0.05):大鼠血清PⅢNP和HA水平,阿苯达唑组和阿苯达唑+黄芪组(30.470 μg/L、100.240 μg/L)较未治疗组明显降低(P<0.05),与阿苯达唑组比较,阿苯达唑+黄芪组大鼠血清TBIL、pⅢNP和HA水平下降更明显(P<0.05).结论 大鼠感染华支睾吸虫后肝功能异常,经阿苯达唑联合黄芪治疗后肝功能得到改善,肝纤维化减轻,阿苯达唑联合黄芪治疗效果好于单独应用阿苯达唑治疗.     

毛里培尼亚保盖凯地小学生埃及血吸虫感染调查

埃及血吸虫病在毛里塔尼亚流行比较广泛,对人民健康威胁很大,为了解其感染情况,我们于2002年10月27日至11月7日,在保盖、凯地两地小学生中开展了埃及血吸虫病调查,现报告如下。1材料与方法1.1样本采集毛里塔尼亚保盖、凯地各小学校学生尿液,随机抽样调查850人。1.2方法1.2.1病原学诊断将尿样放入离心管内适量,离心1000~1500r/min,3~5min弃去尿液,取底部沉淀物0.3~0.5ml,涂于载玻片上低倍镜下观察[1],查到埃及血吸虫卵为诊断依据。1.2.2血尿检测将试纸条放入尿样离心管内,检测尿液中是否存在血尿,1min后观察结果[2]。2结果共调查两地小学生85…     

囊虫病患者尿液中循环抗体与循环抗原的检测

目的探讨检测尿液中囊虫循环抗原和抗体对囊虫病的诊断价值。方法用单克隆抗体夹心-ELISA法检测囊虫病患尿液和血清中循环抗原。间接ELISA法检测尿液和血清中特异性抗体。结果40例囊虫病患尿液中循环抗原阳性率为42．5％,特异性抗体的阳性率为47．5％,30例健康对照均为阴性。结论检测尿液中囊虫病循环抗原和特异性抗体方法简便,为一种非损伤性的囊虫病诊断方法。