阿米三嗪萝巴新片治疗血管性痴呆28例临床分析

1临床资料1.1一般资料本组的血管性痴呆(vascular dementia,VD)患者28例,男性17例,女性11例,年龄58～74岁,平均年龄(65.8±13.5)岁.既往有高血压病史者20例,有冠心病史者10例.     

重组腺病毒介导突变型低氧诱导因子1α基因转染脂肪间充质干细胞并促进其增殖

BACKGROUND:To improve the survival rate of transplanted tissue, most scholars focus on cell therapy, particularly cell-assisted fat grafting. OBJECTIVE:To analyze the effect of recombinant adenovirus-mediated hypoxia inducible factor 1alpha (HIF1α) mutant on survival rate of transplanted fat particles through transfection of adipose-derived mesenchymal stem cells. METHODS:Recombinant adenovirus-mediated triple-mutant HIF1α was inserted into an adenovirus pAdEasy-1 system, followed by viral packaging and titer determination. Human adipose-derived mesenchymal stem cells were cultured, passaged and identified, and subsequently transfected with three kinds of viruses and blank vector (experimental group with transfection of the triple mutant of HIF1α; positive control group; negative control group; blank control group). Transfection efficiency was determined using enhanced green fluorescent protein labeling. Additionally, MTT assay was used to detect cell proliferation. RESULTS AND CONCLUSION:The recombinant adenovirus was successfully constructed and packaged in line with transfection requirements. Moreover, adipose-derived mesenchymal stem cells were successfully identified by adipogenic, osteogenic and chondrogenic induction and could be used as seed cells for subsequent experiments. RT-PCR results showed that HIF1α mRNA expression in the experimental group and positive control group was significantly higher than that in the other two groups(P < 0.05). Western blot analysis showed that the relative absorbance value in the experimental group was significantly higher than that in the other three groups (P < 0.05). A significant increase in the cell proliferation was found in the experimental group, significantly different from the other three groups (P < 0.05). Therefore, our findings indicate that transfection of adenovirus-mediated triple-mutant HIF1α not only can sustain the expression of target protein in transfected adipose-derived mesenchymal stem cells under normoxic conditions, but also can promote the proliferation of transfected adipose-derived mesenchymal stem cells.     

结核性脑膜炎MRI脑膜病变形态和信号特点及颅内继发性改变特征分析

目的分析结核性脑膜炎(TBM)磁共振成像(MRI)脑膜病变形态、信号的特点及颅内继发性改变的发生情况。方法将本院126例TBM作为研究对象,均进行MRI平扫与增强扫描检查,并且其MRI影像表现与42例肺癌脑膜转移患者进行比较。结果 126例TBM患者中,MRI平扫阳性115例(91.27%),包括脑膜中重度增厚91例,轻度增厚24例;阴性11例(8.73%),脑沟、脑池及脑室未见狭窄,并无明显异常信号,MRI增强扫描出现病变处脑沟、脑池内面线状强化高信号。MRI增强扫描均为阳性。脑血管受累54例(42.86%),脑积水81例(64.29%),脑神经受累33例(26.19%)。TBM与肺癌脑膜转移患者均存在脑膜增厚的表现,同时出现不均匀或均匀稍长或等T_1、T_2信号,MRI增强扫描病变脑膜出现强化,结节具有环形、均匀强化的表现。结论 TBM患者MRI影像特点多表现在大脑基底池脑膜增厚,脑膜结节在T_2加权像存在低信号;MRI增强扫描结节有分隔状与环形强化等表现,同时出现继发性改变如脑内前循环血管炎、脑积水及脑梗死等,其对TBM的诊断有着重要的意义,MRI增强扫描对TBM具有重要的诊断价值。     

面部多发癌肿1例报告

1 病例介绍某女,33岁,以鼻根部肿物5年,左面部肿物4个月,快速生长来院求治.入院检查:患者一般状况良好,各常规检查未见异常.右内眦鼻根部可见一约4cm×3cm肿物,表面菜花样,少许脓性分泌物,右下睑轻度外翻.左耳前面部可见同样大小包块,表面光滑,可活动,质中等.     

人本管理思想在护理管理中的应用

在医院管理中,护理人员的服务质量直接影响到医院的医疗质量。随着社会的发展,管理模式开始从物本管理向人本管理转变,人本管理成为了新兴管理理论的前沿,受到了众多学者的关注。如何加强对护理人员的管理,使之有效的应用而产生良好的工作效果是十分重要的。但就目前的护理管理来看,要发挥护理工作者的创造性,激发他们的潜能,还要做许多的努力。其中,实     

雷贝拉唑联合硫糖铝治疗活动性胃溃疡78例

目的研究探讨雷贝拉唑联合硫糖铝治疗Hp感染活动期胃溃疡的临床疗效。方法选取2010年10月-2012年8月期间我院收治的活动性胃溃疡患者156例,随机分为观察组和对照组,各78例。第1周两组患者均接受标准3联疗法根除Hp,第2-8周观察组给予雷贝拉唑联合硫糖铝;对照组给予雷贝拉唑治疗。疗程结束后行胃镜及病理检查,随诊所有患者1年溃疡复发率。结果治疗8周后,两组患者的临床症状和溃疡情况均得到显著改善,组问差异无统计学意义（P〉O．05）;观察组在1年内的溃疡复发率（14．1％）明显低于对照组（35．9％）,差异有统计学意义（P〈0．05）。结论雷贝拉唑联合硫糖铝治疗活动胜胃溃疡安全有效,复发率低,值得临床推广应用。     

RP-HPLC法测定银菊感冒胶囊中氨基比林的含量

目的:建立高效液相色谱法测定银菊感冒胶囊中氨基比林含量的方法.方法:色谱柱采用依利特Hypersil ODS2 C18(4.6 mm ×250 mm,5μm);流动相为乙腈-0.05 moL/l枸橼酸溶液(用三乙胺调节pH至3.5)(10∶90,V/V);流速为1.0 mL/min;进样量为10 μL;柱温为35℃;检...     

潞党参药理实验研究

目的：观察潞党参提取液对小鼠体重、胸腺、血细胞、药物致眠时间的影响。方法：给小鼠自由饮用潞党参提取液15～20d和灌胃10d,观察测量相关体征和生理功能。结果：药液组与对照组有显著性差异。结论：潞党参有补中益气、健脾益肺、抗疲劳等功效。     

青岛市基本公共卫生服务均等化实现途径

在确定基本公共卫生服务供给均等化标准的基础上,测算青岛市基本公共卫生服务差异化的程度,剖析其中的原因,提出从财力、人力、物力、技术等方面完善青岛市基本公共卫生服务均等化的保障机制。