Enhanced CD3+/CD4+ T cell activities and modulation of Th1/Th2 lineage development in irradiated rats due to treatment with the male zooid of antheraea pernyi extracts

Objective  Cancer patients undergoing large dose radiotherapy exhibit multifaceted defects in their immune capacity that are likely to contribute to an increased susceptibility to infections and disease progression. The immune impairment may also constitute a barrier to effective immunotherapeutic interventions. Here, we evaluate whether supplementation with the male zooid of Antheraea pernyi extracts could enhance immune function in irradiated rats. Methods  Fifty male Wistar rats were randomly divided into a control group, a simple radiation group and a treatment group. The mice in the simple irradiation and treatment groups were given whole-chest irradiation with 6Gy. In the treatment group, the male zooid of Antheraea pernyi extracts was gavaged at the doses of 16.53mg/kg (large dose group), 2.62mg/kg (medium dose group), and 0.564mg/kg (small dose group) once a day for 14 days. The thymus and spleen indices were calculated. T cell subsets in peripheral blood were determined by flow cytometry and the expressions of IL-2, IFN-γ, IL-4 and IL-10 in sera were determined by ELISA on the 15th day. Results  The thymus index and spleen index of the high dose treatment group were statistically lower than that of the control group and higher than that of the radiation group (P<0.01). CD3+ and CD4+ T cells in the peripheral blood were increased in the high dose treatment group and decreased in the radiation group (P<0.01). Expression of IL-2 and INF-γ in the radiation group was lower than that in control, and significantly increased during therapy. The production of IL-4 and IL-10 could be induced by radiation and was inhibited in the high dose treatment group (P<0.01). Conclusion  Our data indicate that the male zooid of Antheraea pernyi extracts may be administrated to improve immune function in irradiated rats and reverse the radial immune inhibition of rats by stimulating the proliferation of Th cells and inducing the differentiation of Th2 to Th1. This work was supported by the grants from the National Natural Science foundation of China (No. 30472260), the Administration of Traditional Chinese Medicine of Shandong Province, China(No. 2005-109),, and the Health Protection Committee of Shandong Province, China(No. 2006059).     

青蒿素对大鼠血管平滑肌肌张力的影响

青蒿素(artem isin in)是从菊科植物黄花蒿中提取分离到的一种具有过氧桥的倍半萜内酯类化合物。实验表明,该药具有抗疟、抗孕、抗纤维化、抗血吸虫、抗弓形虫、抗心律失常和肿瘤毒性等作用[1,2]。高血压病是指未查出明确病因而以非特异性循环动脉压持续升高为特点的病理过程,常伴有不同程度心、脑、肾的损害。传统的抗高血压药物主要有4个类型,即利尿剂、β-受体阻断药、血管紧张素转换酶(ACE)抑制剂和钙拮抗剂。研制高效、长效、多器官保护和低副作用的抗高血压药已成为人们关注的热点[3]。青蒿素是否有抗高血压作用,目前尚不清楚。本实…     

血清胱抑素C、尿微量白蛋白和尿β_2-MG联合检测在肝硬化早期肾损害中的应用价值

血清胱抑素(Cys C)、和尿微量白蛋白(MA)、尿β2-微球蛋白(β2-MG)测定已被广泛用于各种原发性或继发性肾脏病变的实验室诊断,可检出尿常规阴性的轻微肾损害,而且特异性和敏感性较高[1-2].肝硬化可以累及肾脏,严重者可引起肝肾综合征(HRS),发生肾功能衰竭.因此,早发现、早期诊断、及时治疗,遏制肾损害、防止肾功能恶化十分重要.     

冠状动脉慢血流现象与心电图运动负荷试验相关性研究

冠状动脉慢血流现象无论是发病原因、机制还是临床表现都不十分清楚。冠状动脉造影仍然是发现这—现象的唯一有效手段。有报道显示心脏运动负荷实验和心肌核素扫描有助于这类患者病情的评价。为明确心脏运动负荷实验对评价冠状动脉慢血流现象的价值，本文对负荷实验与冠状动脉慢血流的关系进行了分析研究。报告如下。     

金果榄浸液治疗输液性静脉炎78例

静脉用药是治疗各种疾病的重要途径之一,而静脉炎又是临床常见的并发症,它不仅增加患者痛苦,还直接影响了治疗.笔者自1994年8月至今,采用金果榄浸液外敷治疗输液性静脉炎78例,疗效满意,现报告如下:     

超声波与高电位氧化还原水协同杀菌作用的实验研究

为了观察超声波与酸性氧化电位水对微生物的协同杀灭作用,采用悬液法在实验室进行了试验研究。结果,单独使用超声波处理10m in,对大肠杆菌杀灭率为89.9%;单独使用酸性氧化电位水作用30 s,对大肠杆菌杀灭率为100%;超声波与氧化水协同作用15 s,杀灭率亦达到100%。单用超声波处理10 m in、单独用酸性氧化电位水作用1.5 m in,可将悬液内HBsAg阳性血清的抗原性完全灭活,二者协同作用仅需30 s即可达到完全灭活。结论,超声波与酸性氧化电位水具有明显协同作用。     

替罗非班在急性心肌梗死介入治疗中的临床研究

目的观察急性心肌梗死(AMI)患者经皮行冠状动脉介入治疗(PCI)时替罗非班的有效性及安全性。方法 80例已确诊的AMI患者PCI术前随机分为替罗非班组和对照组,观察两组PCI术后即刻梗死相关动脉肌梗死溶栓心肌组织灌注(TMP)分级情况、24 h及30 d内主要不良心脏事件(MACE)发生率、出血事件发生率。结果替罗非班组TMP3级发生率高于对照组,24 h及30 d内MACE发生率低于对照组,差异有统计学意义(P<0.05);替罗非班组出血事件发生率高于对照组,但差异无统计学意义(P>0.05)。结论替罗非班能够改善心肌组织再灌注,减少不良心脏事件,疗效优于常规治疗,可安全用于AMI介入治疗中。     

无上消化道症状的阿司匹林相关上消化道出血临床分析

目的 探讨由于应用阿司匹林(ASP)引起上消化道出血而无明显上消化道症状患者的临床及内镜特点.方法 回顾性分析近3年来因服用ASP后出现上消化道出血而无明显上消化道症状患者37例的临床及内镜资料.结果 37例患者中,高龄患者(≥65岁)31例(83.8%),并存原发性高血压病26例(70.3%),服用ASP过程中均未应用抗溃疡药物预防,幽门螺杆菌感染率为56.8%.15例患者接受内镜下治疗,2例内镜下止血失败接受手术治疗,3例患者因心脑血管并发症死亡,余治愈.结论 服用ASP的老年患者,上消化道症状与出血无明显相关性,服药前宜评估其风险,并根治幽门螺杆菌及加用抗溃疡药物.