参芪复方治疗糖尿病阳痿30例

目的:观察参芪复方治疗糖尿痛阳痿的临床疗效.方法:60例糖尿病阳瘘患者随机分为对照组和治疗组各30例,对照组采用前列地尔和甲钴胺治疗,治疗组采用中药治疗,所有病例均采用糖尿痛饮食疗法、口服降糖药或胰岛素控制血糖.两组均治疗30 d.结果:对照组30例中治愈2例,显效5例,有效6例,无效17例,总有效率为43.3％;治疗组30例中治愈4例,显效6例,有效11例,无效9例,总有效率为70.0％.两组总有效率比较,差异有显著性(P＜0.05).结论:参芪复方治疗糖尿痛阳瘘有一定优势.     

阳痿肾阳虚证同证异治浅析

阳痿多辨证为肾阳虚证,一般治疗以补肾为主,但不同疾病所致阳痿疗效不同,故阳痿当同证异治,根据不同疾病导致的阳痿,采用不同治法,不能局限于同证同治,方可获效.     

红景天多糖对精原干细胞体外增殖的影响

BACKGROUND: To establish a rapid and effective method to obtain sufficient spermatogonial stem cells that can meet the clinical need is urgent to be solved in the spermatogonial stem cell transplantation. OBJECTIVE: To study the effect of rhodiola polysaccharide on the proliferation of spermatogonial stem cells in vitro. METHODS: Under sterile conditions, spermatogonial stem cells and Sertoli cells were isolated from the testis of mice, and spermatogonial stem cells were seeded onto the feed layer of Sertoli cells. Then, the co-cultured cells were assigned into experimental group 1 (simple cell culture medium), experimental group 2 (cell culture medium containing 150 mg/L rhodiola polysaccharide) and experimental group 3 (cell culture medium containing 150 mg/L rhodiola polysaccharide, 1 U/L leukemia inhibitory factor and 10 μg/L glial cell line-derived neurotrophic factor). After 7 days of co-culture, flow cytometry was used to detect cell proliferation in vitro, and cell viability and positive expression of GFRa-1, Thy-1 and C-kit were calculated. RESULTS AND CONCLUSION: After 7 days of co-culture, the cells grew rapidly and presented with colony and thyrsiform growth, and the number of cell masses increased significantly, all of which were in line with the proliferative features of spermatogonial stem cells. The GFRa-1, Thy-1 and C-kit proteins were expressed in the cell membrane and cytoplasm, mainly in the cell membrane. The viability of spermatogonial stem cells and positive expression of GFRa-1 and Thy-1 were ranked as follows: experimental group 3 > experimental group 2 > experimental group 1, and there were significant differences between groups (P < 0.05). The positive expression of C-kit had no difference between experimental groups 1 and 2, but it was significantly higher in the experimental group 3 than the other two groups (P < 0.05). These findings indicate that rhodiola polysaccharide used alone or combined with leukemia inhibitory factor and glial cell line-derived neurotrophic factor can enhance the proliferative ability of spermatogonial stem cells in vitro.  中国组织工程研究杂志出版内容重点：干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程     

宫颈绒毛管状腺癌1例报道并文献复习

宫颈绒毛管状腺癌(villoglandular papillary adenocarcinoma,VGPA)是1989年由Young和Scully首次提出的[1].VGPA是宫颈癌中少见的组织病理学类型,是宫颈腺癌分化及愈后较好的一种亚型.VGPA发病率约占宫颈腺癌的3.7％ ～4.8％[1].现将我院收治的1例VGPA报道如下,并复习相关文献. 1 病例简介 患者,女,31岁,因"接触性出血半年"于2012年10月15日收入院.患者,18岁月经初潮,5天/30天,G1P1,4年前经阴顺产1女;无口服避孕药史,无毒物、放射线接触史,无烟酒等不良嗜好.     

治崩三法在血精中的运用

血精是泌尿男科门诊常见的症状之一,诊断及治疗手段多样化,但目前临床缺乏完整、系统规范且行之有效的辨证论治思路。笔者在《丹溪心法附余》学识治崩三法,同时结合辨证论治血精的临床经验,明确"塞流""澄源""复旧"不仅是治疗血精的基本治则治法,而且是血精一病行之有效的辨证论治思路。塞流即急则治标,截流止血,固摄精微;澄源即辨证求因,审因论治,澄本清源;复旧即固本善后,通畅元真,阴阳调和。临床施治当在辨证论治与整体观指导下,灵活运用此三法,急性期治疗以"塞流为标,澄源为本",慢性期治疗以"澄源为标,复旧为本"。     

改良盆底重建术治疗女性盆腔脏器脱垂的临床研究

目的:探讨改良盆底重建术和经阴道子宫切除术(TVH)+阴道前后壁修补术治疗盆腔脏器脱垂性疾病的临床意义。方法:回顾分析2005年6月～2008年2月60例盆腔脏器脱垂(POP)患者行改良盆底重建术(研究组)或TVH+阴道前后壁修补术(对照组)的疗效和并发症发生情况。研究组30例,包括研究1组TVH+改良盆底重建术20例,研究2组单行改良盆底重建术4例,研究3组子宫切除术后POP行改良盆底重建术6例;对照组30例。结果:(1)手术时间、术中出血量、平均住院时间,研究1组与对照组无明显差异(P>0.05);术后保留尿管时间、术后并发症发生率,研究组与对照组差异无显著性(P>0.05);(2)术后临床疗效比较:4组患者出院前测评POP-Q,分度均为0度或Ⅰ度。术后对照组阴道长度较术前明显缩短,研究组阴道长度则较术前无明显变化(P<0.05);(3)随访:术后随访平均8个月,研究组随访率100%,对照组随访率96.67%。POP-Q分度Ⅱ度及Ⅱ度以上判定为复发,研究组1例,复发率为3.33%,对照组5例复发(16.67%)(P<0.05);研究组3例出现性交不适,对照组7例诉性生活质量下降(P<0.05)。结论:TVH+阴道前后壁修补术和改良全盆底重建术均是治疗POP的有效方法。改良盆底重建术作为一种新术式,能更好地修补缺陷、实现结构重建和组织替代,其复发率低,短期疗效稳定,长期疗效有待进一步观察。     

中药治疗慢性前列腺炎系统评价再评价

目的 评价中药治疗慢性前列腺炎的系统评价、Meta分析的方法学质量和证据质量,为临床决策提供参考。方法 对PubMed、Embase、Web of Science、中国知识资源数据库(CNKI)、中国学术期刊数据库(万方数据)和维普中文科技期刊数据库(VIP)中2022年7月27日以前的中药治疗慢性前列腺炎的系统评价、Meta分析进行检索。分别采用AMSTAR 2清单和GRADE评分系统进行方法学质量评价和证据质量分类。结果 共纳入11项系统评价/Meta分析。结果显示,中药治疗慢性前列腺炎的有效性、美国国立卫生研究院慢性前列腺炎症状指数(National Institutes of Health chronic prostatitis symptom index, NIH-CPSI)评分和前列腺液白细胞计数(expressed prostatic secretion-white blood cells, EPS-WBC)指标均优于单纯使用西药;AMSTAR 2量表评价显示,纳入研究的文献方法学质量均很低。证据质量等级分类结果显示,在33项结局指标中,有32项低等级或极低等级证据,只有...     

阴茎角1例报道

检索国内文献，报告阴茎角的不多，且绝大部分有包皮过长或包茎病史，行包皮环切术后发生。阴茎角的具体发病原因不清，可能与人乳头瘤病毒16型感染有关，手术创伤可能是其诱因。我科曾收治1例，报告如下。     

Foley's尿管促晚孕妇女宫颈成熟及其引产效果观察

Ｆｏｌｅｙ′ｓ尿管促晚孕妇女宫颈成熟及其引产效果观察郓城县人民医院（２７４７００）安丘市人民医院山东医科大学附属医院张桂莲程霞张培海宫颈不成熟是晚期妊娠妇女引产失败的主要原因。自１９９７年以来，我们将Ｆｏｌｅｙ′ｓ三腔尿管用于宫颈不成熟晚孕妇的引产过...