造血系统恶性肿瘤患者病毒感染的防治

病毒感染足造血系统恶性肿瘤患者发病和死亡的重要原因.这些患者发生病毒感染的危险取决于T细胞介导的免疫抑制的强度和持续时间、同种移植受者免疫缺陷的程度,与干细胞的采集、供受者匹配情况、预处理方案、药物剂量以及移植物抗宿主病(GVHD)的发生和严重度等有关.     

高剂量造血干细胞移植治疗重型再生障碍性贫血的临床观察

重型再生障碍性贫血(SAA)患者在确诊后的6个月内,如未进行有效治疗,死亡率在50%以上[1]。造血干细胞移植(HSCT)是治疗SAA最有效的方法之一。如何提高SAA患者HSCT的成功率非常重要。我们应用异基因造血干细胞移植(allo HSCT)成功治疗4例SAA患者,报道如下。资料与方法1.病例:从2000年12月至2005年1月间,我们对确诊的4例SAA患者进行了allo HSCT,患者及相应HLA配型完全相合的同胞供者资料见表1。2.供者造血干细胞的动员和采集:病例1、2的供者应用人重组粒细胞集落刺激因子(rhG CSF)300μg/d×5d,皮下注射。当外周血CD34 细胞百…     

川芎嗪对小鼠骨髓基质细胞内皮抑素表达的影响

目的探讨同基因骨髓移植(BMT)小鼠骨髓基质细胞内皮抑素(endostatin,ES)的表达及川芎嗪对其影响。方法取健康BALBc小鼠,随机分为3组正常组(不做处理),骨髓移植对照组(BMT组)和骨髓移植川芎嗪治疗组(川芎嗪组)。BMT组和川芎嗪组分别胃饲生理盐水02ml只和川芎嗪注射液每次2mg只,2次d。在BMT后第7、14、21、28天处死小鼠,采用免疫组化、Westernblot方法检测骨髓基质细胞中ES蛋白表达水平,RTPCR检测骨髓基质细胞中ESmRNA表达水平。结果BMT后第7、14、21、28天川芎嗪组和BMT组ESmRNA和蛋白表达水平均显著低于正常组(P<005或P<001),随着时间延长其表达逐渐增高。BMT后第14、21、28天川芎嗪组ES表达水平始终低于BMT组(P<005);但骨髓有核细胞计数高于BMT组(P<005或P<001)。结论川芎嗪能反馈调节BMT小鼠骨髓基质细胞新血管生成抑制因子的表达,促进骨髓微环境的修复,加速移植后骨髓的造血重建。     

放射损伤小鼠骨髓单个核细胞表面bFGFR、Flt-1的表达

目的　探讨放射损伤对小鼠骨髓单个核细胞表面bFGFR、Flt 1表达的影响。方法　流式细胞仪检测照射后昆明小鼠骨髓单个核细胞表面bFGFR、Flt 1表达水平的变化。结果　放射损伤后小鼠骨髓单个核细胞表面bFGFR、Flt 1表达水平显著低于正常组 (t =2 33～ 6 35 ,t =4 14～2 0 6 1,P <0 0 1～P <0 0 5 ) ,随时间延长 ,bFGFR、Flt 1表达逐渐增高 ,但 14d时仍稍低于正常组 (t=2 33～ 4 17,P <0 0 5～P <0 0 1) ;骨髓单个核细胞表面bFGFR表达与Flt 1表达呈正相关 (r =0 5 5 33,P <0 0 5 )。结论　 6 0Gy6 0 Coγ射线可损伤骨髓单个核细胞表面bFGFR、Flt 1的表达 ,进一步说明辐射所致骨髓微环境损伤与骨髓组织中bFGF、VEGE表达异常有关。     

Effect of Ligustrazine on Expressions of Basic Fibroblast Growth Factor and Its Receptor in Bone Marrow of Mice with Acute Radiation Injury

Objective: To study the expressions of basic fibroblast growth factor (bFGF) and its receptor (bFGFR) in bone marrow of mice with acute radiation injury, and to evaluate the effect of Ligustrazine (Lt) on them. Methods: Fifty-six Kunming mice of clean grade were randomly divided into 3 groups, the normal group, the control group and the Lt group. Mice in the latter two groups were once homogeneously systemic irradiated with 6.0 Gy of 60 Co, with the absorption dose rate of 0. 56 Gy/min, then treated with saline (0.2 ml/ mice) or Lt (2 mg/mice) respectively, twice a day through gastrogavage for successive 13 days. Mice were sacrificed in batch on the 3rd, 7th and 14th day by cervical dislocation to collect the bilateral femoral bone marrow for preparing bone marrow mono-nuclear cell (BMMNC) suspension. The bFGFR expression on surface of BMMNC was determined by flow cytometry; and the bFGF expres-sion level in one side of femoral bone marrow tissue was detected by immunohistochemistry with SABC-AP assay. R     

探讨依托咪酯复合舒芬太尼在电子肠镜检查中的应用

目的:观察依托咪酯复合小剂量舒芬太尼用于无痛电子结肠镜检查的安全性及临床效果。方法:将我院肛肠科于2014年1月-6月接受电子结肠镜检查及治疗的患者420例.自愿分为对照组和研究组。其中对照组124例,研究组296例。对照组未给予任何止痛措施。研究组患者静脉先给予舒芬太尼0.08~0.10μg/kg。1分钟后按0.2mg/kg静脉注射依托咪酯实施麻醉。比较两组患者检查过程中出现的恶心、呕吐、腹部疼痛、肢体扭动等不适情况。结果:研究组均安全顺利地完成结肠镜检查。观察组患者有明显的恶心呕吐,腹部疼痛等不良反应。结论:依托咪酯复合小剂量舒芬太尼用于无痛电子结肠镜检查是舒适无痛、安全有效的。     

Effects of Ligustrazine on Hematopoiesis in the Early Phase of Bone Marrow Transplantation Mice

the National NaturalScience Foundation(Serial No.3 9870 92 6)sacrificed on the1 st,3rd,5 th,7th,1 0 th,1 5 th,and 2 1 st day after BMT.The marrow from fe-murs was flushed into RPMI- 1 640 medium. BM-NCs from each femur were counted.1 .2 .7Carbon- Mold Preparation of Blood Vessels　　 A colloidal carbon solution( prepared Chineseink,0 .2 ml,2 0 mg/ml in saline) was injected1 -2 min before sacrifice on the1 st,3rd,5 th,7th,1 0 th,1 5 th,2 1 st day after BMT.One femur ofeach mouse was r…     

大颗粒淋巴细胞白血病的诊断及治疗

大颗粒淋巴细胞白血病(LGLL) 是一起源于CD3+CTL 和CD3-NK 细胞的克隆性增殖性疾病, 以肿瘤细胞胞 浆中含有嗜苯胺蓝颗粒为特征。该病临床表现有惰性和侵袭性之分。其诊断需结合患者临床表现以及大颗粒淋巴 细胞的形态学、免疫表型、分子遗传学等指标来确立。对于惰性的无临床症状的LGLL 患者只需进行随访观察, 无 须治疗, 对于有症状者推荐行免疫抑制治疗。对于侵袭性LGLL 患者, 急性淋巴细胞白血病样化疗加异基因造血干 细胞移植可能是有效治疗。     

妊娠合并特发性血小板减少性紫癜

特发性血小板减少性紫癜（ITP）是一种以外周血小板数目持续减少为特征的自身免疫性疾病。在成人中好发于40岁以下育龄女性,且不影响生育功能,所以妊娠合并ITP是临床医生经常遇到的问题,其机率报道不一,约为1～2／1000名妊娠妇女,     

Enhancing Effects of Ligustrazine on Expression of CD31 and Hematopoietic Reconstitution in Syngenic Bone Marrow Transplantation of Mice

Summary： The effect of ligustrazine on the expression of CD31 in syngenic bone marrow transplantation （BMT） mice was studied. Fifty-six Balb/c mice were divided into 3 groups： normal control group. BMT control group, and ligustrazine treated group. Syngenic BMT mouse models were established according to the literatures. In BMT control group and the ligustrazine treated group, the mice were given respecxively orally 0.2 mL saline and 2 mg ligustrazine twice a day. On the 7th, 14th, and 21st day after BMT, the mice were killed. The expression of CD31 on the surface of bone marrow nuclear cells （BMNC） was detected by flow cytometry. Peripheral blood leukocytes, platelets and BMNC were counted. Histological observation of bone marrow was made. The results showed thai in ligustrazine treated group the peripheral blood leukocylcs, platelets and BMNC counts, and the expression of CD31 on the day 7, 14, 21 after BMT were higher than in BMTcontrol group （P〈0.01 or P〈0.05）. In conclusion, ligustrazine could obviously enhance the CD31 expression on the surface of BMNC after syngcnic BMT in mice, which may be one of the mecha- nisms underlying the ligustrazine accelerating hematopoietic reconstitution in syngenic BMT.